Vesicular stomatitis virus RNA: Complementarity between infected cell RNA and RNA's from infectious and autointerfering viral fractions

Characterization of Snap-back RNAs in Vesicular Stomatitis Defective Interfering Virus Particles

“…passages following cloning (Reichmann, Pringle & Follett, 197I ;Stampfer, Baltimore & Huang, 1971 ;Perrault & Holland, i972a;Holland, Villarreal & Breindl, I976a). These shorter truncated or T particles contain deleted forms of the genome or B RNA (Huang & Wagner, 1966;Brown et al 1967;Schaffer, Hackett & Soergel, 1968;Roy & Bishop, 1972) which specifically inhibit the replication of the latter (Huang & Manders,I972;Perrault & Holland,I972b).…”

“…Some VSV T particles contain a mixture of plus and minus strand RNA whereas others appear to contain minus strand RNA only, as in the case of the infectious particle (Schaffer et al 1968;Roy & Bishop, 1972;Roy et al 1973;Leamnson & Reichmann, 1974;Stamrninger & Lazzarini, 1974;Schnitzlein & Reichmann, I976). Recently, we and others have shown that a particular clone of VSV gave rise to a T particle (referred to as DIe5 in our previous study and as C5 ST in this paper) which contains covalently linked plus and minus strands (Lazzarini et al I975;Perrault,I976).…”

Viruses, Polymerases, and Cancer

“…As we were beginning to unravel the multiple RNA's of VSV, Schaffer et al (32) published a paper showing that the major VSV-induced RNA's in infected cells, like those induced by Sendai and Newcastle disease viruses, were complementary in base sequence to the virion RNA. We confirmed and extended that observation, showing that the virusspecific RNA recovered from the polyribosomes of infected cells (the viral messenger RNA) was all complementary to virion RNA (33).…”

Animal RNA Viruses: Genome Structure and Function

“…It appears not to function directly as a messenger (65,(79)(80)(81)(82)(83) and contains little, if any, poly(A) (25,87,85). In infected cells, the viral genome is transcribed into shorter complementary sequences.…”

“…Class 2 genome RNA transcripts are present in polyribosomes of infected cells and probably function as monocistronic viral mRNA (80,81,83,98). Virus-specific RNA molecules large enough to be polycistronic mRNA are also present in VSV-and NDV-infected cells, and post-translational cleavage of nascent viral structural poly-.…”