Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Abstract

KEY WORDS:Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de alpacas (Vicugna pacos)Viability in vitro and invivo of sperm frozen/thawed from alpaca (Vicugna pacos) vas deferens El objetivo del presente estudio fue evaluar la viabilidad in-vitro e in-vivo de los espermatozoides procedente de los conductos deferentes de alpacas. Se utilizaron 2 reproductores como donadores de espermatozoides con desviación del conducto deferente. Los espermatozoides colectados se sometieron a la congelación y descongelación con el dilutor Triladyl®. Durante el procesamiento de los espermatozoides se evaluaron la motilidad y la prueba hipo-osmotica. En la inseminación se evaluó la proporción de gestaciones producidas. Los resultados fueron los siguientes: La motilidad de los espermatozoides para los reproductores 1 y 2 fueron: a los 37°C 65.16 y 63.37% sin diferencia (P>0.05), al enfriamiento (5°C) 52.63 y 48.31% similares (P>0.05) ya la descongelación 24.51 y 33.18% mostraron diferencia (P<0.05). A la prueba hipo-osmótica fueron; a los 37°C 51.55 y 52.98% similares (P>0.05), Al enfriamiento (5°C) 46.67 y 55.93% mostraron diferencia (P<0.05) y a la descongelación 20.06 y 32.18% con diferencia (P<0.05). Las gestaciones fueron: Con espermatozoides frescos diluidos 36.36% (4/11), con espermatozoides descongelados 25.00% (5/20) y con monta 54.54% (6/11), siendo similares (P>0.05). En conclusión los espermatozoides procedentes de los conductos deferentes soportan el congelamiento y a la inseminación artificial producen gestaciones.The aim of this study was to evaluate the in-vitro and in-vivo viability of sperm from the vas deferens alpacas. 2 males as sperm donors were used to offset the vas deferens. The collected sperm cells were subjected to freezing and thawing with dilutor Triladyl®. During processing of sperm motility and hypo-osmotic test they were evaluated. Insemination in the proportion of pregnancies produced was evaluated. The results were as follows: The sperm motility of males 1 and 2 were: at 37 ° C 65.16 and 63.37% with no difference (P> 0.05), cooling (5 ° C) 52.63 and 48.31% similar (P > 0.05) and thawing 24.51 and 33.18% showed no difference (P <0.05). The hypo-osmotic test were; at 37 ° C 51.55 and 52.98 similar percentage (P> 0.05), and cooling 55.93% 46.67 showed no difference (P <0.05) and thawing 20.06 and 32.18% with difference (P <0.05). Pregnancies were diluted with fresh sperm 36.36% (4/11), with defrosted sperm 25.00% (5/20) and 54.54% mounts (6/11), being similar (P> 0.05). In conclusion sperm from the vas deferens withstand freezing and insemination are able to produce pregnancies.

Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders

“…This study collected alpaca semen by postcoital aspiration, and an extender based on Tris-egg yolk was used, obtaining an average range of refrigerated sperm motility from 57.3% to 61.5%. On the other hand, Pérez Durand et al (2016) reported motility values somewhat lower than those observed in our study, after alpaca sperm was cooled to 5°C in a period of 3 h (mean ranging from 48.3 to 52.6%). Even though they use the commercial extender Triladyl®, the authors collected samples from the deviation of the vas deferens.…”

“…Among the multiple available commercial options for semen extenders, several of them have been used to freeze alpaca sperm, although none of them are SAC-specific (Green/clear camel buffer; Biladyl® A and B;Triladyl®;, and with low pregnancy rates (0% to 25%) after AI implementation (Morton et al, 2010;Pérez Durand et al, 2016;Stuart et al, 2019;Vaughan et al, 2003).…”

“…Nonetheless, the current goal is to develop semen protocols with commercial extenders that are easy to use, have less variation in composition providing reproducible results, and require fewer equipment and reagents (Bertuzzi et al., 2022). Among the multiple available commercial options for semen extenders, several of them have been used to freeze alpaca sperm, although none of them are SAC‐specific (Green/clear camel buffer; Biladyl® A and B; Triladyl®; and INRA‐96), and with low pregnancy rates (0% to 25%) after AI implementation (Morton et al., 2010; Pérez Durand et al., 2016; Stuart et al., 2019; Vaughan et al., 2003). To our knowledge, AndroMed® has never been used for alpaca semen freezing and subsequent AI.…”

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

“…This option is too severe for the animal and does not allow repeated sampling. The alternative technique used in this study is the best option for removing both limitations (Gómez‐Quispe, Pérez, & Machaca, ; Pérez Durand, Zevallos Aragón, & Pérez Guerra, ).…”

“…There are previous studies focused on alpaca spermatozoa without seminal plasma, which were collected post mortem from the epididymis (Choez et al, ) and with the same procedure used in this work (Gómez‐Quispe et al, ; Pérez Durand et al, ; Perez et al, ). Yet, the subjective analysis of total motility showed higher values than our results.…”