Viability and function of human sperm in electrolyte-free cold preservation

Kanno, Hitomi; Saito, Kazuo; Ogawa, Takehiko; Takeda, Mitsumasa; Iwasaki, Akira; Kinoshita, Yuzo

doi:10.1016/s0015-0282(97)00439-1

Cited by 11 publications

(4 citation statements)

References 23 publications

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“…Previous attempts to preserve human sperm in electrolyte-free medium were directed towards the goal of repeated insemination. Although sperm motility was maintained for up to 4 weeks [Kanno et al 1998;Saito et al 1996], the total number of motile sperm after storage was not assessed. Compared to ICSI, inseminations and IVF require high numbers of sperm to achieve fertilization.…”

Section: Discussionmentioning

confidence: 99%

Freezing-Free Preservation of Human Spermatozoa – A Pilot Study

Riel

Huang

Ward

2007

Archives of Andrology

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This study tested a method for maintaining human spermatozoa without freezing for subsequent use in intracytoplasmic sperm injection (ICSI). We demonstrated that human sperm stored in electrolyte-free solution maintain their motility and viability for at least 4 and 6 weeks, respectively. We also have shown that preserved spermatozoa are fully functional in ICSI. Sperm chromosome analysis after injection of human sperm into mouse oocytes revealed that two weeks of storage does not negatively affect sperm DNA integrity. A mouse model was used to analyze the ability of preserved sperm to participate in normal embryogenesis. Mouse sperm preserved in electrolyte-free solution in a similar manner as human sperm maintained motility for up to 3 weeks. When mouse spermatozoa stored for 1 week were injected into the oocytes by ICSI, they yielded normal blastoctysts and normal viable fetuses. The results of the study bear significance for human assisted reproduction technologies (ART) and provide clinicians and infertile patients with a new method that can simplify sperm preparation for ICSI, assisting men who are unable to provide semen on the day of assisted fertilization.

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Section: Discussionmentioning

confidence: 99%

Freezing-Free Preservation of Human Spermatozoa – A Pilot Study

Riel

Huang

Ward

2007

Archives of Andrology

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“…Results showed that preserving sperm in EFM at 4 °C could maintain motility of sperm from both normal and asthenozoospermic semen samples for at least weeks, much better than solutions containing sodium or potassium and TEST-yolk buffer. Follow-up studies showed that the morphology, viability, ATP concentration, and acrosome status of sperm were also conserved in EFM cold preservation [ 124 , 267 ]. Besides, the chromatin integrity of sperm preserved using this method after one week was maintained better than cryopreservation and were proven to be fully functional in ICSI [ 11 , 34 ].…”

Section: Typical Extenders Used To Store Human Spermatozoa In Liquid ...mentioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

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Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role in reproductive biology and medicine. However, it is well known that human spermatozoa left in vitro deteriorate over time irreversibly as the consequence of various stresses such as the change of osmolarity, energy deficiency, and oxidative damage, leading to substantial limitations including the need for semen examinations, fertility preservation, and assisted reproductive technology. These problems may be addressed with the aid of non-freezing storage techniques. The main and most effective preservation strategies are the partial or total replacement of seminal plasma with culture medium, named as extenders, and temperature-induced metabolic restriction. Semen extenders consist of buffers, osmolytes, and antioxidants, etc. to protect spermatozoa against the above-mentioned adverse factors. Extended preservation of human spermatozoa in vitro has a negative effect on sperm parameters, whereas its effect on ART outcomes remains inconsistent. The storage duration, temperature, and pre-treatment of semen should be determined according to the aims of preservation. Advanced techniques such as nanotechnology and omics have been introduced and show great potential in the lifespan extension of human sperm. It is certain that more patients will benefit from it in the near future. This review provided an overview of the current knowledge and prospects of prolonged non-freezing storage of human sperm in vitro.

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“…Both glycolysis and the Krebs cycle play an important role in sperm energy metabolism. Sperm from various species stored in a range of solutions, osmolarities and storage temperatures, were shown to be functional when inject into oocytes after storage of weeks to several months (Kanno et al, 1998;Van Thuan et al, 2005;Riel et al, 2007;Riel et al, 2011). An alternative is to simply leave the spermatozoa inside the epididymides and keep these at 4ºC.…”

Section: Liquid Phase Semen Short-to Mid-term Storagementioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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Viability and function of human sperm in electrolyte-free cold preservation

Cited by 11 publications

References 23 publications

Freezing-Free Preservation of Human Spermatozoa – A Pilot Study

Freezing-Free Preservation of Human Spermatozoa – A Pilot Study

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Genome Banking for Vertebrates Wildlife Conservation

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