Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation

Kommisrud, Elisabeth; Myromslien, Frøydis Deinboll; Stenseth, Else‐Berit; Zeremichael, Teklu Tewoldebrhan; Hofman, Nadine; Grevle, Inger S.; Sunde, Jan

doi:10.1016/j.theriogenology.2020.04.008

Cited by 23 publications

(17 citation statements)

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“…In addition, the percentage of motile sperm is influenced by the addition of extenders and cryoprotectants. [57][58][59] However, sperm motility from fresh semen was slightly greater compared to cryopreserved semen from Esox lucius. 50 The fertility rate of eggs ranged from 76 and 84%; however, no significant correlation was detected between fertility rate and sperm parameters, such as semen volume, semen pH, motility, and duration of motility.…”

Section: Discussionmentioning

confidence: 95%

Reproductive characteristics of the giant gurami sago strain (Osphronemus goramy Lacepède, 1801): basic knowledge for a future hatchery development strategy

2021

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Background: The giant gourami sago strain (Osphronemus goramy Lacepède) has been approved in 2018 as a candidate for freshwater aquaculture in Indonesia. However, information on the species’ reproduction is minimal. This study analyzed the reproductive characteristics of the gourami sago strain broodfish to provide basic knowledge for a future hatchery development strategy. Methods: A total of 10 female and 10 male mature gourami sago strain broodfish were measured for body weight and length, and were evaluated for their reproductive characteristics. Breeding fish were spawned naturally in a 2×1×0.6 m concrete pond with a male-female sex ratio of 1:1. Egg weight and diameter were measured in 25 eggs per female using, respectively, ACIS AD- 600i scales with 0.01 g accuracy, and a microscope (Labo model L-711) using Canon Digital Camera Software 3 . Semen was collected using plastic syringes in 3 mL aliquots, then placed in an insulated ice-cooled container, and analyzed within two hours of collection. Results: Average weights of female and male broodfish before spawning were 2180±159.78 g and 3060±134.99 g, respectively. The relative fecundity and egg diameter were 1029±36 eggs kg-1 and 2.42±0.05 mm, respectively. The hatching rate and embryo survival to an eyed-egg stage were respectively 76.40±2.27% and 94.76±0.42%. Sperm characteristics showed that volume was 0.60±0.12 ml kg-1 and percentage of motile sperm was 70.04±2.27%. Female broodfish weight after spawning was strongly correlated with the weight before spawned (r2 = 0.999) and absolute fecundity was also strongly correlated with female broodfish weight before spawning (r2= 0.921). Sperm concentration was moderately correlated with sperm motility (r2 = 0.556) and duration of sperm motility (r2 = 0.502). Conclusions: The gourami sago strain broodfish has suitable reproductive characteristics for the development of hatcheries. Successful natural spawning should be followed by larval weaning and feeding technology to increase growth and survival.

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Section: Discussionmentioning

confidence: 95%

Reproductive characteristics of the giant gurami sago strain (Osphronemus goramy Lacepède, 1801): basic knowledge for a future hatchery development strategy

2021

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“…Proteomic studies also reveal carbonylation of sperm proteins (Mostek et al, 2018). On the other hand, negative effects on motility may due to losses of intracellular ATP (De Baulny et al, 1997;Kommisrud et al, 2020) and mitochondrial membrane potential. Both loss of proteins and ATP could be explained by the rupture of the cell and mitochondrial membrane (Lahnsteiner et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of coporo ( Prochilodus mariae ) milt using three permeating cryoprotectant agents and two freezing systems

et al. 2021

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In the present study, we examined three permeating cryoprotectant agents (CPAs) and two freezing systems for the cryopreservation of coporo spermatozoa. Milt samples of 5 males were diluted with cryoprotectant solutions containing 10% Me 2 SO, 10% MeOH and 8% DMA, respectively, with or without hen egg yolk. Half of the 0.25-ml straws were frozen in a programmable system and the other half in a dry shipper.Sperm motility and viability were evaluated in both fresh and post-thaw samples by conventional methods. Sperm motility and viability were both significantly lower in all post-thaw treatments. Of the CPA tested, 10% MeOH provided the best protection as there were no significant differences in motility (66.67 ± 5.77% vs. 66.68 ± 5.70%) and viability (80.00 ± 4.35% vs. 83.33 ± 0.57) between the programmable system and the dry shipper when that CPA was used. On the other hand, the most effective freezing system was the programmable freezer as all the CPA tested presented sperm motility (43.33 ± 5.77 to 66.50 ± 5.00%). In conclusion, coporo sperm can be cryopreserved in either of these two systems using 10% methanol.

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“…The quality of cryopreserved sperm may be affected by a number of factors including collection and handling procedures prior to preservation (Kommisrud et al, 2020), as well as the cryopreservation protocol being used. Cryopreservation may produce damage in cells, which affects plasma membrane, mitochondria, and chromatin structure with potential implications to fertilization success (Cabrita et al, 2010;Figueroa et al, 2016;Mayer, 2019).…”

Section: Obtaining Sufficient and Predictable Sperm Quality And Quantity In/from Cryopreserved Miltmentioning

confidence: 99%

“…For example, Figueroa et al (2016Figueroa et al ( , 2018 demonstrated fertilization rates of 81.6% ± 1.9% and 80.3% ± 3%, respectively compared to about 93% in fresh milt controls. The cryopreservation protocol developed by Cryogenetics ® and used by the NGB was tested by Kommisrud et al (2020) and demonstrated a high and predictable fertilization rate of 73.9 ± 1.7% rate using cryopreserved milt of Atlantic salmon compared to 81.1 ± 1.2% for fresh milt controls (Kommisrud et al, 2020). Similar results were obtained for cryopreserved milt from Arctic charr in which no difference in fertility rate was detected between cryopreserved milt performed by Cryogenetics and fresh milt (Grevle, Ritter, & Sunde, 2015).…”

Section: Obtaining Sufficient and Predictable Sperm Quality And Quantity In/from Cryopreserved Miltmentioning

confidence: 99%

“…is ambient temperature and proper handling during storage and shipment prior to cryopreservation. Previous work demonstrates that storage under temperatures around 5-6 C for less than 3 days ensures that fertilization success is not compromised prior to preservation (Kommisrud et al, 2020). In NGB, collected samples are therefore placed on ice immediately after collection, and shipped to a cryopreservation laboratory after a maximum of 2 days following collection.…”

Section: Obtaining Sufficient and Predictable Sperm Quality And Quantity In/from Cryopreserved Miltmentioning

confidence: 99%

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Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks

Bøe

Bjøru

Bårdsen

et al. 2021

Conservat Sci and Prac

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Atlantic salmon are facing population declines and loss of productivity within populations due to anthropogenic impact factors and reduced survival at sea. Biobanking is an increasingly used tool to conserve the genetic integrity and diversity of populations threatened by extirpation. The aim of the current article is to discuss the opportunities and challenges that increased use of cryopreservation brings to biobanking activities, using the Norwegian Gene Bank (NGB) for Atlantic salmon as a model system. The NGB was established in 1985 and involves a traditional living gene bank, as well as "frozen gene bank"

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Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation

Cited by 23 publications

References 50 publications

Reproductive characteristics of the giant gurami sago strain (Osphronemus goramy Lacepède, 1801): basic knowledge for a future hatchery development strategy

Reproductive characteristics of the giant gurami sago strain (Osphronemus goramy Lacepède, 1801): basic knowledge for a future hatchery development strategy

Cryopreservation of coporo ( Prochilodus mariae ) milt using three permeating cryoprotectant agents and two freezing systems

Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks

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