Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

Carvalho, Sara Monaliza Costa; Torres, Salvador Barros; Benedito, Clarisse Pereira; Nogueira, Narjara Walessa; Souza, Antônia Adailha Torres; Neta, Maria Lilia de Souza

doi:10.1590/2317-1545v39n1163784

Cited by 15 publications

(20 citation statements)

References 14 publications

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“…In the tetrazolium test, when the seed is immersed in the tetrazolium solution, it diff uses through the tissues, causing a reduction reaction in the living cells, which results in a red, non-diff usible compound, triphenylformazan, indicating that there is respiratory activity in the mitochondria and, consequently, the viability of the tissue (live) (Oliveira et al, 2018). There is a clear separation of living tissue, which breathes, from dead tissue that does not stain, and consequently does not breathe (Carvalho, 2016).…”

Section: Discussionmentioning

confidence: 99%

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

et al. 2019

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Studying the physiological and biochemical behavior of Myrcianthes pungens seeds stored in different packages for up to ten months was the objective of this work. Seeds were collected in Toledo, Pato Bragado, and Marechal Cândido Rondon and stored in a cold and dry chamber (11 ºC and 6,3% RH) for ten months. The germination test and the germination speed index (GSI), the seedling length (SL), the seedling dry matter mass (DMS), and the tetrazolium (ZT) test were performed and, for each evaluation, the water content was determined. Non-parametric analysis was used. During storage, the water content of the seeds in the plastic and glass containers varied little, but decreased in the paper packaging. The germination was maintained for ten months when the seeds were stored in plastic containers and for two months in the glass and paper ones. The variables GSI, SL, and DMS presented upwards and downwards variations during the storage period. The ZT revealed that, in the plastic packaging, the seeds remained viable for up to ten months, in glass, up to two months, and in paper, up to four months. Therefore, seeds of Myrcianthes pungens with high initial quality can be stored in a cold and dry chamber in plastic bags, maintaining viability and vigor for a period of ten months

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Section: Discussionmentioning

confidence: 99%

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

et al. 2019

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“…A temperatura de hidratação e de embebição em solução de tetrazólio pode ter influenciado na penetração da solução nas sementes. Sabe-se que o fluxo de água para dentro da semente na fase I do padrão trifásico da germinação ocorre por diferença de potencial matricial com o ambiente, esse processo é puramente físico, quando em conjunto com o aumento da temperatura do ar, contribui para aumentar a viscosidade da água e, consequentemente, aumenta a velocidade de absorção da água pela semente (CARVALHO et al, 2017).…”

Section: Tratamentosunclassified

Viabilidade De Sementes De Castanha-Do-Brasil Pelo Teste De Tetrazólio

Borella

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Souza

et al. 2020

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Objetivou-se revisar e propor o protocolo de viabilidade das sementes da Bertholletia excelsa pelo teste de tetrazólio. Sementes oriundas de plantio comercial propagado por enxertia com 16 anos de idade implantadas na Fazenda Iracema em Cláudia, MT, foram destegumentadas e cortadas longitudinalmente, depois foram hidratadas em água por imersão direta (ID) e papel toalha Germitest® (PG) por 24 e 48 h e embebidas nas concentrações de 0,1 e 0,5% do sal de tetrazólio por 12 e 24 h no escuro à 30 °C. O delineamento experimental foi completamente randomizado em fatorial 2 x 2 x 2 x 2 (forma de embebição x tempo em embebição x tempo em solução de tetrazólio x concentração da solução de tetrazólio) com quatro repetições de 8 sementes. O teste de tetrazólio foi eficiente para colorir e identificar as sementes viáveis e inviáveis. A avaliação da viabilidade das sementes da B. excelsa destegumentadas e partidas ao meio foi melhor na condição de embebição com imersão direta em água por 24 h e concentração de 0,5% do sal tetrazólio por 24 h à 30 °C e embebição em papel toalha Germitest® por 24 h e concentração de 0,5% do sal tetrazólio por 12 h à 30 °C.Palavras-chave: Bertholletia excelsa Humb. Bonpl; potencial fisiológico; sementes florestais; recalcitrância. VIABILITY OF BRAZIL NUTS SEEDS BY TETRAZOLIUM TEST ABSTRACT: The objective was to review and propose the protocol of viability of Bertholletia excelsa seeds by tetrazolium test. Seeds from the commercial planting propagated by grafting with 16-year-old implanted at Fazenda Iracema in Cláudia, MT, were undegumented and cut longitudinally, then hydrated in water by direct immersion (ID) and Germitest® paper towel (PG) for 24 and 48 h and soaked in concentrations of 0.1 and 0.5% of tetrazolium salt for 12 and 24 h in the dark at 30 ° C. The experimental design was completely randomized in a factorial design 2 x 2 x 2 x 2 (soaking form x soaking time x time in tetrazolium solution x tetrazolium solution concentration) with four replications of 8 seeds. The tetrazolium test was efficient to color and identify viable and unviable seeds. The evaluation of viability of detegumented and cut longitudinally B. excelsa seeds was better in: direct soaking in water for 24 h and concentration of 0.5% of tetrazolium salt for 24 h at 30 ° C and soaking in Germitest® paper towels for 24 h and a concentration of 0.5% of the tetrazolium salt for 12 h at 30 ° C.Keywords: Bertholletia excelsa Humb. Bonpl; physiological potential; forest seeds; recalcitrance.

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“…var. ferrea, is a Brazilian tree found mainly in the north and northeast regions, and is commonly known as Jucá or pau-ferro (5,6). In the Amazon, Jucá is extensively used in popular medicine to treat various health conditions, including bronchopulmonary diseases, diabetes, rheumatism, cancer, gastrointestinal disorders, and diarrhea, in the form of tea and infusions.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Phytopharmaceuticals From the Amazonian Plant Libidibia ferrea for Wound Healing in Dogs

et al. 2020

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We comparatively evaluate two distinct formulations containing 5% of Jucá (Libidibia ferrea) for wound healing in dogs. An excision model study was performed in 11 dogs with three dermal wounds in each animal, which were treated with: (1) topical phytopharmaceutical based on Carbopol (PyC) containing 5% Jucá ethanolic extract; (2) topical phytopharmaceutical based on Astrocaryum murumuru butter (PyM) containing 5% Jucá ethanolic extract; and (3) commercial ointment (control). Wound treatment was carried out on alternated days starting at day (D) one until D21. Macroscopic (all time-points) and histological (D0 and D21) analyses were performed. The antimicrobial activity of Jucá was evaluated through Minimal Inhibitory Concentration (MIC). Phytochemical analysis of Jucá revealed 3.1% phenolic compound content expressed in rutin and the presence of hydrolyzable tannins and flavonoids. The mean wound retraction was 33.7 ± 5.5, 34.0 ± 4.7, and 28.4 ± 4.9 % for PyC, PyM, and control groups, respectively, with higher wound retraction for both herbal-treated groups compared to the control (P < 0.05). Alcoholic extract of Jucá had antimicrobial activity against the microorganisms Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida krusei at different degrees, with MIC ranging from 250 to 16.625 µg/ml. Microscopic evaluation showed that the phytotherapic formulations contributed to better dermal wound healing through wound fibroplasia. The alcoholic extract of Jucá pods has great potential for wound healing in dogs and can be used in the development of commercially viable phytotherapic formulations.

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Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

Cited by 15 publications

References 14 publications

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

Viabilidade De Sementes De Castanha-Do-Brasil Pelo Teste De Tetrazólio

Efficacy of Phytopharmaceuticals From the Amazonian Plant Libidibia ferrea for Wound Healing in Dogs

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