Vibrating Sample Magnetometer for Protein Research

Redfield, Alfred G.; Moleski, Charles

doi:10.1063/1.1685752

Cited by 11 publications

(3 citation statements)

References 8 publications

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“…We were the first to notice this source of irreproducibility in susceptibility studies (2). It very probably was the noise source limiting previous metalloprotein studies, which had a noise level corresponding to a concentration of ± ±0.2 mM spin 1/2 electrons (20,22,23). That the magnitude of the slowly relaxing component of the proton's nuclear magnetization varies from sample to control makes physical techniques for dealing with this problem impractical.…”

Section: Spin L/2 Nucleimentioning

confidence: 99%

SQUID measurement of metalloprotein magnetization. New methods applied to the nitrogenase proteins

Day¹,

Kent²,

Lindahl³

et al. 1987

Biophysical Journal

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New techniques have been developed to exploit the sensitivity of a commercial SQUID susceptometer in the study of the magnetization of metalloproteins. Previous studies have ignored both the slow relaxation (hours) of spin I = 1/2 nuclei and residual ferromagnetic impurities in sample holders. These potential sources of noise were at or below the sensitivity of previous instruments. With these noise sources under control, one can now decrease the protein concentration by a factor of ten. In addition careful characterization of the frozen magnetization sample, including the use of a multi-instrument holder for combined study of the magnetization sample with Mössbauer spectroscopy, is required for reliable interpretation of the data in the face of paramagnetic impurities common to metalloprotein samples. Many previous magnetic studies of metalloproteins have been carried out in the Curie region. Saturation magnetization studies down to 1.8 K and up to 5 T can determine zero-field splitting parameters in addition to the spin and exchange coupling parameters measured in previous studies at lower fields and higher temperatures. Applications of these techniques to the study of the nitrogenase proteins of Azotobacter vinelandii are presented as examples.

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Section: Spin L/2 Nucleimentioning

confidence: 99%

SQUID measurement of metalloprotein magnetization. New methods applied to the nitrogenase proteins

Day¹,

Kent²,

Lindahl³

et al. 1987

Biophysical Journal

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“…Magnetic susceptibility measurements at temperatures below 77°K were made with a vibrating sample susceptometer (Redfield and Moleski, 1972) as described previously (Moss et al, 1969), except that deoxygenation with glucose-glucose oxidase was omitted. The dissolved oxygen correction for the experiments was estimated by measuring the susceptibility of air-equilibrated buffer.…”

Section: Methodsmentioning

confidence: 99%

Magnetic properties of Cancer magister hemocyanin

Th¹,

Dc²,

Ehrenberg³

et al. 1973

Biochemistry

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“…A simple vibrating sample magnetometer (VSM) for work with weakly paramagnetic frozen solutions is described by Redfield and Moleski (65). We earlier reviewed (52, 53) important aspects of work in protein research carried out by Moss, Moleski, and coworkers.…”

Section: Instrumentationmentioning

confidence: 99%

Magnetic susceptibility. Trends in instrumentation and applications to solid state science

Mulay¹,

Mulay²

1974

Anal. Chem.

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In this seventh review on magnetic susceptibility, we survey important trends in instrumentation and applications, especially in the realm of solid state science. The first six reviews (46, 49-53) appeared during 1962 to 1972. This 1974 addition covers literature from about January 1972 to December 1973. In response to an editorial plea, we have made this review more concise than the previous ones. In doing so, it seemed imperative that we should depict the exceptionally novel trends and eliminate those that we covered before, without, of course, implying in the least that these are no longer important. Hence, we shall not review the work on lunar samples, which was adequately covered in 1970 and 1972 (52, 53) and work on transition metal complexes including the biocomplexes, which we surveyed in all reviews (46, 49-53) up to now. It should be noted that excellent surveys on the transition metal complexes are now available and are listed in a

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Vibrating Sample Magnetometer for Protein Research

Cited by 11 publications

References 8 publications

SQUID measurement of metalloprotein magnetization. New methods applied to the nitrogenase proteins

SQUID measurement of metalloprotein magnetization. New methods applied to the nitrogenase proteins

Magnetic properties of Cancer magister hemocyanin

Magnetic susceptibility. Trends in instrumentation and applications to solid state science

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