Vibrational Imaging Based On Stimulated Raman Scattering Microscopy

Beyond the borders — Biomedical applications of non-linear Raman microscopy

“…By contrast SRS signals are detected as a loss or gain in the intensity of one of the two exciting lasers. This means that whereas, just as in the case of fluorescence microscopy, the CARS signal can be separated from the excitation light by means of a simple optical filter [20], the detection of a SRS signal requires more demanding lockin detection [21,22]. The detection in CARS microscopy is therefore similar to other multiphoton techniques such as two-photon excited fluorescence emission and/or second (third) harmonic generation signals.…”

Fiber-laser-based Stimulated Raman microspectroscopy with shot-noise limited sensitivity

“…Stimulated Raman scattering (SRS) microscopy [1][2][3] is a powerful technique for label-free biomedical imaging because SRS provides chemical contrast based on intrinsic vibrational response of sample molecules with subcellular spatial resolution. Recently, there are two important research trends in SRS microscopy.…”

SRS microscopy as a low-invasive 3D bioimaging technique

“…In particular, stimulated Raman scattering (SRS) microscopy gives molecular-specific images with high sensitivity, high contrast and quantitativeness [7][8][9]. The theoretical sensitivity limit of SRS microscopy [8] is as high as that of CARS microscopy [3], which can be operated even at the video rate [4].…”

“…Coherent nonlinear-optical microscopy [1][2][3][4][5][6][7][8][9], which exploits nonlinear-optical interaction induced by focused ultrafast laser pulses, has been regarded as powerful candidate for label-free, 3D visualization of biological samples. In particular, stimulated Raman scattering (SRS) microscopy gives molecular-specific images with high sensitivity, high contrast and quantitativeness [7][8][9].…”