Vibrio alginolyticus associated with white spot disease of Penaeus monodon

Abstract

In February 2000, white spot disease outbreaks occurred among cultured Penaeus monodon in extensive shrimp farms on the southwest coast of India. Bacteria were isolated from infected shrimp that showed reddish body coloration and white spots in the cuticle. The isolates were screened on thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. The primary isolate (QS7) was characterized as V. alginolyticus based on morphological, biochemical and physiological characteristics. Antibiotic sensitivity tests of QS7 indicated that the isolate was highly sensitive to chloramphenicol, ciprofloxacin, nalidixic acid and streptomycin. Pathogenicity tests confirmed that the isolate was virulent for P. monodon. Based on the lethal dose (LD 50 ) value (5 × 10 6 cfu per shrimp), it was inferred that shrimp weakened by white spot syndrome virus would succumb to secondary infection by QS7.

Investigations on white spot disease outbreak in Penaeus monodon (Fabricius, 1798) in association with Vibrio mimicus infection in the Sunderbans, West Bengal, India

“…Therefore, the co-infection of V. mimicus and WSSV in the pond was ascertained. Four species of bacteria viz., Vibrio alginolyticus, V. parahaemolyticus, V. anguillarum and Pseudomonas aeruginosa were so far reported, in association with WSD in shrimps (Jayasree et al, 2000, Joseph Selvin andLipton, 2003;Liu et al, 2004). Results of the present study showed that V. mimicus infection can also cause severe mortality in association with WSD.…”

“…Clinical signs and mortality pattern were observed every 15 min during the first hour post-injection (hpi) followed by every 1 h until 6 hpi. The experiment was continued for seven days and the animals were monitored every 12 h (Joseph Selvin and Lipton, 2003). Parameters such as pH, temperature and salinity were recorded thrice a day for seven days.…”

Expression, purification, and characterization of thermolabile hemolysin (TLH) from Vibrio alginolyticus

“…V. alginolyticus is a gramnegative, estuarine bacterial species and is the closest relative of V. parahaemolyticus (González-Escalona et al 2006, Cai et al 2007. V. alginolyticus has been described as an important pathogen, and is responsible for the ulcer disease of gilthead sea bream Sparus aurata, turbot Scophthalmus maximus, crimson snapper Lutjanus erythopterus, sea mullet Mugil cephalus, and giant freshwater prawn Macrobrachium rosenbergii (Kim et al 2000, Selvin & Lipton 2003, Jayaprakash et al 2006, Cai et al 2007. Previous studies have shown that V. alginolyticus is a major infective pathogen for silver sea bream Sparus sarba, an edible fish of major economic value to Hong Kong (Li et al 2003).…”

Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases

“…In Asia, among the pathogenic Vibrio group, 11 species were reported from the shrimp culture systems (Lavilla-Pitogo, 1995). V. alginolyticus and V. harveyi poses a serious disease problem in cultured black tiger shrimp in India (Karunasagar et al, 1997;Selvin & Lipton 2003a;Manilal et al, 2010b). Vibrio species are considered to be members of the normal bacterial flora of shrimp and the culture environment (Jiravanichpaisal et al, 1994;Otta et al, 1999).…”