Vibrio cholerae high cell density quorum sensing activates the host intestinal innate immune response

Jugder, Bat-Erdene; Batista, Juliana H.; Gibson, J A; Cunningham, Paul; Asara, John M.; Watnick, Paula I.

doi:10.1016/j.celrep.2022.111368

Cited by 16 publications

(10 citation statements)

References 71 publications

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“…Sample preparation was adapted from Jugder et al 90 . Briefly, 400uL of culture was harvested by centrifugation, then subjected to extraction in 800uL 80% Methanol for 20 minutes in an ultrasonicator bath (Elmasonic P, Elma) at 20°C.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting supernatant was dried in a SpeedVac Vacuum Concentrator (Thermo) under vacuum and ambient temperature, then stored at -20°C. Samples were analyzed by the Beth Israel Deaconess Medical Center Mass Spectroscopy Core Facility as per previous studies 90 using a 6500 Qtrap triple quadrupole mass spectrometer (AB/SCIEX), Prominence UFLC HPLC (Shimadzu), and selected reaction monitoring (SRM) of 298 water-soluble metabolites using MultiQuant v3.0 software (AB/SCIEX). The resulting peak areas (Supplemental Data 6) were analyzed and visualized using Metaboanalyst.ca, using normalization to median, log10 transformation, and mean-centering functions.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

Schubert,

Tang,

Goodchild-Michelman

et al. 2023

Preprint

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Cyanobacteria are photosynthetic organisms that play important roles in carbon cycling as well as promising bioproduction chassis. Here, we isolate two novel cyanobacteria, UTEX 3221 and UTEX 3222, from a unique marine environment with naturally elevated CO₂. We describe complete genome sequences for both isolates and, focusing on UTEX 3222 due to its planktonic growth in liquid, characterize biotechnologically-relevant growth and biomass characteristics. UTEX 3222 outpaces other fast-growing model strains on solid medium. It can double every 2.35 hours in a liquid medium and grows to high density (>31g/L biomass dry weight) in batch culture, nearly double that ofSynechococcussp. PCC 11901, whose high-density growth was recently reported. In addition, UTEX 3222 sinks readily, settling more quickly than other fast-growing strains, suggesting improved de-watering of UTEX 3222 biomass. This settling behavior can be explained in part by larger cell volume. These traits may make UTEX 3222 a compelling choice for photosynthetic bioproduction from CO₂. Overall, we find that bio-prospecting in environments with naturally elevated CO₂ may uncover novel CO₂-metabolizing organisms with unique characteristics.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

Schubert,

Tang,

Goodchild-Michelman

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Regarding pathogenicity, HapR binds to promoters to repress the expression of CT and TCP. Regarding immune evasion, HapR reduces bacterial tryptophan uptake, thus providing host enterocytes with precursors for serotonin synthesis that activate innate immune signaling (36). In brief, Vibrio cholerae exploits flagellar motility, ToxT-mediated transcriptional changes, TCP, and CT to colonize the intestine, induce diarrhea, and evade immune detection through microcolony and biofilm formation.…”

Section: Pathogen and Pathogenesismentioning

confidence: 99%

Cholera: A Latent Threat to Human Health

Alaridah,

Shewaikani,

Al Refaei

et al. 2024

Arch Clin Infect Dis

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: Cholera outbreaks caused by the bacterium Vibrio cholerae present a significant global health challenge, with a notable increase in cases recently reported. The disease is characterized by severe watery diarrhea, leading to dehydration and potential fatalities if not promptly addressed. Transmission occurs through contaminated food and water sources, underscoring the importance of water sanitation and hygiene measures to prevent outbreaks. Limited healthcare access and inadequate reporting systems make estimating cholera cases and deaths challenging. Antibiotic resistance is also a concerning issue, necessitating the development of new treatment options. Prompt laboratory diagnosis is essential, with rapid diagnostic tests and PCR showing promise for pathogen detection. Treatment involves fluid replacement and appropriate antibiotic use to reduce disease severity and transmission. Oral cholera vaccines offer preventive measures for high-risk individuals during outbreaks. To combat the escalating cholera epidemic and save lives, a comprehensive approach, including improved water sanitation, early detection, and timely treatment, is crucial.

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“…HapR also represses expression of Vibrio polysaccharide (VPS) matrix enzymes, as demonstrated in a Drosophila host ( Zhu et al, 2002 ; Vance et al, 2003 ; Kamareddine et al, 2018 ). Judger et al suggests that quorum sensing activates HapR to repress tryptophan uptake, which cascades to activate a commensal relationship from the Drosophila host innate immune system via serotonin production ( Jugder et al, 2022 ).…”

Section: Advances In Cellular and Molecular Biology Of The ...mentioning

confidence: 99%

Advances in cholera research: from molecular biology to public health initiatives

et al. 2023

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The aquatic bacterium Vibrio cholerae is the etiological agent of the diarrheal disease cholera, which has plagued the world for centuries. This pathogen has been the subject of studies in a vast array of fields, from molecular biology to animal models for virulence activity to epidemiological disease transmission modeling. V. cholerae genetics and the activity of virulence genes determine the pathogenic potential of different strains, as well as provide a model for genomic evolution in the natural environment. While animal models for V. cholerae infection have been used for decades, recent advances in this area provide a well-rounded picture of nearly all aspects of V. cholerae interaction with both mammalian and non-mammalian hosts, encompassing colonization dynamics, pathogenesis, immunological responses, and transmission to naïve populations. Microbiome studies have become increasingly common as access and affordability of sequencing has improved, and these studies have revealed key factors in V. cholerae communication and competition with members of the gut microbiota. Despite a wealth of knowledge surrounding V. cholerae, the pathogen remains endemic in numerous countries and causes sporadic outbreaks elsewhere. Public health initiatives aim to prevent cholera outbreaks and provide prompt, effective relief in cases where prevention is not feasible. In this review, we describe recent advancements in cholera research in these areas to provide a more complete illustration of V. cholerae evolution as a microbe and significant global health threat, as well as how researchers are working to improve understanding and minimize impact of this pathogen on vulnerable populations.

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Vibrio cholerae high cell density quorum sensing activates the host intestinal innate immune response

Cited by 16 publications

References 71 publications

Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

Cholera: A Latent Threat to Human Health

Advances in cholera research: from molecular biology to public health initiatives

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