Vibrio sp. strain NM 10, isolated from the intestine of a Japanese coastal fish, has an inhibitory effect against Pasteurella piscicida

Sugita, Haruo; Matsuo, Norifusa; Hirose, Yuko; Iwato, M; Deguchi, Yoshiaki

doi:10.1128/aem.63.12.4986-4989.1997

Cited by 70 publications

(32 citation statements)

References 13 publications

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“…piscicida. Sugita, Matsuo, Hirose, Iwato & Deguchi (1997) reported similar findings. The isolates characterized as members of the genus Micrococcus did not inhibit the pathogenic strains assayed.…”

Section: Discussionsupporting

confidence: 68%

Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida

Chabrillón

Rico

Balebona

et al. 2005

Journal of Fish Diseases

104

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Most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. However, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. Nineteen isolates obtained from farmed Senegalese sole, Solea senegalensis Kaup, and gilthead sea bream, Sparus aurata L., have been evaluated for their capacity to adhere to skin and intestinal mucus of Senegalese sole, and their antagonistic effect against Photobacterium damselae subsp. piscicida, an important pathogen for farmed sole. The isolates from gilthead sea bream showed the highest percentage of adhesion to sole mucus, whilst the pathogenic microorganisms assayed and the isolates from sole showed, in general, a lower ability to adhere to sole mucus. The results suggest that the adhesion to fish mucus was more dependent on the isolate tested than on the host mucus. The isolates from gilthead sea bream also showed a higher antagonistic activity against P. damselae subsp. piscicida than those from Senegalese sole. Four isolates were selected, on the basis of their adhesive ability and antagonistic effect on P. damselae subsp. piscicida, to study their interactions with the pathogen in respect of adhesion to skin and intestinal mucus under exclusion, competition and displacement conditions. The results obtained show the ability of three isolates to reduce the adhesion of P. damselae subsp. piscicida to sole mucus under displacement and competition conditions. The adhesion of the pathogen to sole intestinal mucus was also significantly reduced when three isolates were assayed under exclusion conditions.

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Section: Discussionsupporting

confidence: 68%

Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida

Chabrillón

Rico

Balebona

et al. 2005

Journal of Fish Diseases

104

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“…Over the past three decades, the intestinal microbiota of fish have been ecologically investigated (Horsley 1977;Cahill 1990;Sugita 1997). The intestinal bacteria produce bioactive substances, such as tetrodotoxin (Noguchi et al 1987), eicosapentaenoic acid (Yazawa et al 1988;Ringø et al 1992), biotin (Sugita et al 1992), vitamin B 12 (Sugita et al 1991 and antibacterial substances (Westerdahl et al 1991;Sugita et al 1997), which might benefit the host fish. These facts suggest a symbiotic relationship between fish and their associated intestinal microbiota (Sugita 1997).…”

Section: Discussionmentioning

confidence: 99%

Identification of intest\inal bacteria from Japanese flounder (Paralichthys olivaceus) and their ability to digest chitin

Sugita

Itō

2006

Lett Appl Microbiol

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“…Smith and Davey (1993) and Austin et al (1995) have reported the possibility that fish diseases may be treated with probiotic strains of bacteria. Effective control of the establishment of pathogenic bacteria in fish intestines by using probiotic strains with the ability to produce antimicrobial substances, including bacteriocins, instead of by chemotherapeutic agents may be essential in aquaculture (Sugita et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported inhibitory effects against some pathogenic Vibrio species from resident microbiota of fish and shellfish (Dopazo et al 1988;Westerdahl et al 1991;Bower et al 1994;Riquelme et al 1996;Ruiz et al 1996;Sugita et al 1996). The inhibitory effect of bacteria is due to the following factors, either singly or in combination: production of antibiotics, bacteriocins, siderophores, lysozymes, proteases, hydrogen peroxide and the alteration of pH values produced by organic acids (Sugita et al 1997;Gibson 1999).…”

Section: Introductionmentioning

confidence: 99%

Vibrios isolated from the cultured manila clam (Ruditapes philippinarum): numerical taxonomy and antibacterial activities

et al. 2002

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Aims: A numerical taxonomic study of halophilic Vibrio isolated from healthy and brown ring disease (BRD) affected manila clams (Ruditapes philippinarum), harvested from the Atlantic coast of south-western Spain, was performed. Methods and Results: Characterization of 123 presumptive Vibrio spp. was carried out using 94 phenotypic tests. Simple matching and Jaccard similarity coefficients were used for numerical analysis. Cluster analysis by the unweighted pair group method with arithmetic averages yielded 15 phena defined at 0AE81 similarity. Large phena corresponded to Vibrio tubiashii, V. splendidus biotype I and V. harveyi (phena 1, 5 and 9, respectively). The species V. splendidus biotype II, V. natriegens, V. mediterranei and V. alginolyticus were also represented. The inhibitory effect of diffusible extracellular products of the isolates against 27 strains of V. tapetis, the aetiological agent of BRD, was also investigated. Only five V. tubiashii isolates inhibited the growth of V. tapetis strains. The antimicrobial effect was inhibited by heating and depended on the culture medium. Conclusions: The main Vibrio species associated with manila clams were V. tubiashii, V. spendidus and V. harveyi. The antagonistic relationship established between V. tapetis and the Vibrio spp. clam microbiota may explain the failure of isolation in plating medium of V. tapetis from BRD-affected clams on the south Atlantic coast of Spain. Significance and Impact of the Study: Some of the strains isolated from manila clams correspond to agarolytic strains that constitute phenon 7 and they do not fit into any of the currently described Vibrio species.

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Vibrio sp. strain NM 10, isolated from the intestine of a Japanese coastal fish, has an inhibitory effect against Pasteurella piscicida

Cited by 70 publications

References 13 publications

Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida

Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida

Identification of intest\inal bacteria from Japanese flounder (Paralichthys olivaceus) and their ability to digest chitin

Vibrios isolated from the cultured manila clam (Ruditapes philippinarum): numerical taxonomy and antibacterial activities

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