Vicilin genes ofPisum

Ellis, Noel; Domoney, Claire; Castleton, J. A.; Cleary, W. G.; Davies, D. R.

doi:10.1007/bf02428047

Cited by 36 publications

(37 citation statements)

References 25 publications

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“…pea Vc-2). In contrast several distinct loci have been described for vicilins and convicilins in pea (Ellis et al 1986;Domoney and Casey 1990;Casey and Domoney 1999). For the Medicago cupin gene cluster, five genes have been annotated within 31 000 bp that includes three hypothetical protein encoding regions: such an arrangement is in agreement with the close genetic linkage within individual loci in pea.…”

Section: Discussionsupporting

confidence: 53%

“…1d) and several VicB amplicons in others (Fig. 1b) is further indication of large deletions affecting entire loci comprising closely linked related genes (Ellis et al 1986). …”

Section: Resultsmentioning

confidence: 84%

“…It is clear that the Cvc deletion mutation affects two closely linked genes (Ellis et al 1986) and that these lines carry an additional morphological mutation, k. So far, our data indicate that the Cvc and k loci are very closely linked with no recombination among 80 recombinant inbred lines, in contrast to an earlier study which reported recombination between these (3.2 AE 1.1 crossover %, Matta and Gatehouse 1982). The latter study was based on F2 populations of over 100 individuals and protein mobility shifts in convicilin.…”

Section: Discussionmentioning

confidence: 99%

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Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics

Domoney

Knox

Moreau

et al. 2013

Functional Plant Biol.

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Abstract.A fast neutron (FN)-mutagenised population was generated in Pisum sativum L. (pea) to enable the identification and isolation of genes underlying traits and processes. Studies of several phenotypic traits have clearly demonstrated the utility of the resource by associating gene deletions with phenotype followed by functional tests exploiting additional mutant sources, from both induced and natural variant germplasm. For forward genetic screens, next generation sequencing methodologies provide an opportunity for identifying genes associated with deletions rapidly and systematically. The application of rapid reverse genetic screens of the fast neutron mutant pea population supports conclusions on the frequency of deletions based on phenotype alone. These studies also suggest that large deletions affecting one or more loci can be non-deleterious to the pea genome, yielding mutants that could not be obtained by other means. Deletion mutants affecting genes associated with seed metabolism and storage are providing unique opportunities to identify the products of complex and related gene families, and to study the downstream consequences of such deletions.

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Section: Discussionsupporting

confidence: 53%

“…1d) and several VicB amplicons in others (Fig. 1b) is further indication of large deletions affecting entire loci comprising closely linked related genes (Ellis et al 1986). …”

Section: Resultsmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

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Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics

Domoney

Knox

Moreau

et al. 2013

Functional Plant Biol.

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“…A cDNA library, prepared from early-to mid-maturation embryo poly(A) RNA from P. sativum cv. Birte (Domoney and Casey 1983), was screened with representatives of all previously characterized clones (Domoney and Casey 1985;Ellis et al 1986) and a number of clones which did not hybridize were selected for further characterization by hybrid-selection/translation (Domoney and Casey 1983) and sequencing.…”

Section: Analysis Of Rnamentioning

confidence: 99%

“…Purification of DNA, restriction digests, Southern blot analysis and linkage analysis were as described previously Ellis et al 1986). …”

Section: Analysis Of Rnamentioning

confidence: 99%

A developmentally regulated early-embryogenesis protein in pea (Pisum sativum L.) is related to the heat-shock protein (HSP70) gene family

Domoney

Ellis

Turner

et al. 1991

Planta

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A cDNA clone, pCD7, was shown to hybrid-select from developing seeds of Pisum sativum L. an mRNA that translated into a polypeptide of apparent Mr 90 000. The translation product was observed only in the earliest stages of embryogenesis and was detected at a developmental stage when virtually all the cotyledon cells are mitotic. Sequence analysis of pCD7 showed it to correspond to a member of the 70 000-Mr heat-shock protein (HSP70) gene family. Transcripts corresponding to pCD7 were detected in different P. sativum organs, with roots apparently showing lower levels of pCD7-homologous RNA than other organs. Hybridizations to P. sativum DNA identified polymorphisms in the genomic DNA corresponding to pCD7 and the segregation of these in selected crosses indicated the existence of at least two genetic loci, one of which mapped to an existing linkage group.

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The effects of genetic variation at r, rb and Tri loci in Pisum sativum L. on apparent ileal digestibility of amino acids in young broilers

et al. 2005

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The nutritional effect of genetic variation at r and rb loci was investigated by determining apparent and true amino acid digestibility of four near-isogenic lines of pea (RRRbRb, RRrbrb, rrRbRb, rrrbrb) fed to young broilers in a metabolism study. The results demonstrated that the round-seeded line (RRRbRb) generally had the lowest amino acid content with, for example, 2.5 g cystine kg −1 dry matter (DM) and 1.6 g methionine kg −1 DM, compared with contents in the three wrinkled-seeded lines (rrRbRb, RRrbrb and rrrbrb). The double mutant line (rrrbrb) generally had the highest amino acid content, with 3.2 g cystine kg −1 DM and 2.3 g methionine kg −1 DM, and an elevated amino acid content compared with the single mutant lines (RRrbrb, rrRbRb). However, the round-seeded line (RRRbRb) consistently had the highest coefficient of true (CTID), and apparent (CAID), ileal digestibility for all amino acids (e.g. 0.959 and 0.928 respectively for CTID and CAID for methionine) when compared with the three wrinkled lines RRrbrb, rrRbRb and rrrbrb (0.853, 0.877 and 0.866, respectively, for CTID, and 0.862, 0.882 and 0.874 for CAID). A second experiment examined the digestibility of amino acids in a pair of lines, HC8 and LC8, near-isogenic except for the locus controlling the concentration of seed trypsin inhibitor (H, high; L, low). The results showed that the CAID and CTID obtained for all amino acids were consistently higher for LC8 than for HC8 (for methionine: 0.905 and 0.709 for CTID, respectively, for LC8 and HC8; 0.887 and 0.729, respectively, for CAID), indicating the very specific effects of quantitative variation in these proteins on nutritional value of peas for poultry. INTRODUCTIONPea genotypes show a wide variation in seed composition, reflecting different genetic backgrounds. The genetic loci with the greatest influence on seed composition and, by inference, nutritional value include the r, rb and Tri loci, with major primary effects on starch composition (r, rb) and trypsin inhibitor content (Tri) of mature seeds.Mutations at both the r and rb loci were first identified by their effect on the shape of the mature seed, the presence of either mutation giving the dried seed a wrinkled appearance. A mutant at the r locus has been known for at least 150 years and was used by Mendel. A mutant at the rb locus was first genetically characterised 1 and it was determined that the r and rb mutations were at independent loci. The primary effects of mutations at either or at both of these

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Vicilin genes ofPisum

Cited by 36 publications

References 25 publications

Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics

Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics

A developmentally regulated early-embryogenesis protein in pea (Pisum sativum L.) is related to the heat-shock protein (HSP70) gene family

The effects of genetic variation at r, rb and Tri loci in Pisum sativum L. on apparent ileal digestibility of amino acids in young broilers

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