CP 13-4100' (Reg. no. CV-202, PI 698546) sugarcane cultivar (a complex hybrid of Saccharum spp.) was developed through cooperative research conducted by the USDA-ARS, the University of Florida, and the Florida Sugar Cane League, Inc. The Florida Sugarcane Variety Committee (FSVC) released CP 13-4100 in June 2020 to growers for commercial cultivation on mineral (sand) soils. It originated from a polycross made at Canal Point (CP) on 3 Dec. 2008 where CP 96-1252 was the female parent and the male parent was unknown and could be any one of genotypes used in the polycross. CP 13-4100 was released by FSVC for its high cane and sucrose yields, acceptable commercial recoverable sucrose, and resistances to orange rust, leaf scald, Sugarcane mosaic virus strain E (mosaic), and smut, and acceptable levels of susceptibility to brown rust. CP 13-4100 and commercial reference checks (i.e., CL 88-4730, CP 96-1252, CPCL 97-2730) yield data were collected from 12 harvests (i.e. three crop cycles-plant cane, first, and second ratoon-at four sand-soil locations) in final-stage replicated yield trials. Compared with CL 88-4730 and CPCL 97-2730, CP 13-4100 had significantly (P ≤ .0001) higher cane yield (tonnes of cane per hectare: 18.7 and 29.5%), sucrose yield (tonnes of sugar per hectare: 26.3 and 30.7%), and economic index (EI; 10.9-16.9%) and no significant differences from CP 96-1252 in these traits. CP 13-4100 tested negative for the Bru1 marker, which is linked to a quantitative major locus of brown rust resistance. CP 13-4100 could be considered moderately tolerant to freezing among 23 tested genotypes.