Vincamine production in multiple shoot culture derived from hairy roots of Vinca minor

Abstract

Characteristics of regenerated plants obtained from hairy roots (Ri-transformed plants) of Vinca minor L., a producer of a pharmaceutically important indole alkaloid, vincamine, were investigated. A previously established Ritransformed clone, Vm-101, proliferates rapidly in vitro, displays a high degree of lateral branching and rapid shoot elongation and has a growth index 2.5 times that of an untransformed plant. The addition of 2.2 ~M benzyladenine to the culture medium increased the shoot number but did not decrease the growth index. Vincamine content in the leaves of in vitro-cultured Vm-101 was twice that in the cultured untransformed plant. These results suggest that multiple shoot culture of Ri-transformed plants may be an excellent tool for in vitro vincamine production.

Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone

“…In addition there are several reports on higher concentration of secondary metabolites in plants regenerated from hairy roots in comparison to non-transformed tissue in Ajuga reptans (Tanaka and Matsumoto 1993), Pelargonium spp. (Pellegrineschi et al 1994), Vinca minor (Tanaka et al 1995). In order to obtain higher level of ramentaceone in D. capensis var.…”

Plant-Based Indole Alkaloids: A Comprehensive Overview from a Pharmacological Perspective

“…Vincamine was isolated from the plant Vinca minor [ 53 ]. At doses of 20 and 30 mg/kg, vincamine exhibited significant reductions in the levels of serum triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and very-low-density lipoprotein cholesterol (VLDL-C), and a comparative increase in high-density lipoprotein cholesterol (HDL-C), resulting in the overall improvement of the lipid profile of rats ( Figure 17 ) [ 54 ].…”

Biosynthesis and accumulation of a medicinal compound, Picroside-I, in cultures of Picrorhiza kurroa Royle ex Benth

“…Moreover, the biosynthesis and accumulation of Picroside-I and Picroside-II occur in P. kurroa at high altitudes and that too during a particular time of a season, which complicates the process of understanding biology of their biosynthesis. The cell cultures offer a suitable biological system with a controlled environment wherein the morphogenetic events can be regulated by manipulating the levels of growth hormones in the nutrient medium resulting in rapid production of plant metabolites of pharmaceutical importance (Ray and Jha 2001;Tanaka et al 1995). Tissue cultures of P. kurroa have been done for its rapid multiplication (Lal et al 1988;Upadhyay et al 1989).…”