Viral and Nonviral Elements in Potexvirus Replication and Movement and in Antiviral Responses

Park, Mi-Ri; Seo, Jang‐Kyun; Kim, Kook‐Hyung

doi:10.1016/b978-0-12-407698-3.00003-x

Cited by 22 publications

(18 citation statements)

References 159 publications

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“…However, co-IP assays with anti-P20 IgG ( Figure 6D) or anti-fibrillarin IgG ( Figure 6E) revealed positive signals for TGBp3HA in HV and satBaMV coinfected leaves ( Figures 6D and 6E, lane 6). Hence, consistent with observations in other potexviruses (Park et al, 2013), BaMV TGBps and CP may interact with each other, and P20 may form protein complexes with fibrillarin, BaMV TGB proteins, and CP in plant tissues coinfected with HV and satBaMV.…”

Section: Fibrillarin and P20 Form Complexes With Satbamv Rnasupporting

confidence: 88%

The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants

et al. 2016

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RNA trafficking plays pivotal roles in regulating plant development, gene silencing, and adaptation to environmental stress. Satellite RNAs (satRNAs), parasites of viruses, depend on their helper viruses (HVs) for replication, encapsidation, and efficient spread. However, it remains largely unknown how satRNAs interact with viruses and the cellular machinery to undergo trafficking. Here, we show that the P20 protein of Bamboo mosaic potexvirus satRNA (satBaMV) can functionally complement in trans the systemic trafficking of P20-defective satBaMV in infected Nicotiana benthamiana. The transgenederived satBaMV, uncoupled from HV replication, was able to move autonomously across a graft union identified by RT-qPCR, RNA gel blot, and in situ RT-PCR analyses. Coimmunoprecipitation experiments revealed that the major nucleolar protein fibrillarin is coprecipitated in the P20 protein complex. Notably, silencing fibrillarin suppressed satBaMV-, but not HV-, phloem-based movement following grafting or coinoculation with HV. Confocal microscopy revealed that the P20 protein colocalized with fibrillarin in the nucleoli and formed punctate structures associated with plasmodesmata. The mobile satBaMV RNA appears to exist as ribonucleoprotein (RNP) complex composed of P20 and fibrillarin, whereas BaMV movement proteins, capsid protein, and BaMV RNA are recruited with HV coinfection. Taken together, our findings provide insight into movement of satBaMV via the fibrillarin-satBaMV-P20 RNP complex in phloem-mediated systemic trafficking.

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Section: Fibrillarin and P20 Form Complexes With Satbamv Rnasupporting

confidence: 88%

The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants

et al. 2016

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“…Additional information and alternative interpretations of findings can be found in several other reviews published elsewhere (e.g. Laliberté and Zheng, 2014;Liu and Nelson, 2013;Park et al, 2013;Niehl and Heinlein, 2011;Heinlein, 2015).…”

mentioning

confidence: 91%

Plant virus replication and movement

2015

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Replication and intercellular spread of viruses depend on host mechanisms supporting the formation, transport and turnover of functional complexes between viral genomes, virus-encoded products and cellular factors. To enhance these processes, viruses assemble and replicate in membrane-associated complexes that may develop into "virus factories" or "viroplasms" in which viral components and host factors required for replication are concentrated. Many plant viruses replicate in association with the cortical ER-actin network that is continuous between cells through plasmodesmata. The replication complexes can be highly organized and supported by network interactions between the viral genome and the virus-encoded proteins. Intracellular PD targeting of replication complexes links the process of movement to replication and provides specificity for transport of the viral genome by the virus-encoded movement proteins. The formation and trafficking of replication complexes and also the development and anchorage of replication factories involves important roles of the cortical cytoskeleton and associated motor proteins.

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“…The interaction between HBV continuous replication and antiviral immune response results in chronic hepatitis B viral occurrence [43,44]. The liver also contains numerous specific immune cells whose role in the incidence of HBV infection, development, and prognosis has been increasingly recognized [45,46]. HBV-specific T-cell responses have been associated with self-limited or persistent HBV infections [47].…”

Section: Adaptive Immune Response To Hbvmentioning

confidence: 98%

Gut microbiota modulate the immune effect against hepatitis B virus infection

Huang

Wang

2015

Eur J Clin Microbiol Infect Dis

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The immunological mechanisms by which hepatitis B virus (HBV) initiates and maintains acute or chronic infection, even the formation of cirrhosis and hepatocellular carcinoma, are still undefined. An increasing number of studies have shown that intestinal flora regulate immune homeostasis, and, thus, protect the immunologic function against hepatitis virus infection. In this article, we discuss gut microbiota and its potential immune effects against HBV infection. It may provide a novel insight into the pathogenesis of HBV infection, as well as a potential therapeutic target to HBV-related disease.

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Viral and Nonviral Elements in Potexvirus Replication and Movement and in Antiviral Responses

Cited by 22 publications

References 159 publications

The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants

The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants

Plant virus replication and movement

Gut microbiota modulate the immune effect against hepatitis B virus infection

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