Viral Capsids as Self-Assembling Templates for New Materials

Dedeo, Michel T.; Finley, Daniel; Francis, Matthew B.

doi:10.1016/b978-0-12-415906-8.00002-9

Cited by 39 publications

(36 citation statements)

References 132 publications

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“…There are well-established examples of icosahedral virus CPs self-assembling into empty particles when expressed from a heterologous system such as Escherichia coli and Pichia pastoris (Brumfield et al, 2004;Dedeo et al, 2011;Sainsbury et al, 2010). While we will not describe these established heterologous expression systems in detail we do want to highlight the development of a CPMV system for the generation of empty VLPs directly in plants (Saunders et al, 2009).…”

Section: Strategies For Epvn Production and Controlled Assemblymentioning

confidence: 97%

“…lysine, cysteine, tyrosine, histidine) that provide reactive sites for the chemical conjugation of desired agents which include fluorescent and medical imaging dyes, protein or small molecule therapeutics and reactive peptides for sensor or cell entry (Franzen and Lommel, 2009;Lee et al, 2014;Li and Wang, 2014). Because of limited space we will not attempt to categorize the numerous chemical conjugation methods that have been used to functionalize viral capsids and instead direct the reader to several excellent reviews that cover this topic as well as provide strategies for selecting appropriate chemical conjugation schemes (Dedeo et al, 2011;Stephanopoulos and Francis, 2011;Strable and Finn, 2009).…”

Section: Chemical Conjugationmentioning

confidence: 98%

“…The ability to control and modify the formation of assembly intermediates represents a powerful means to produce novel ePVN structures that would not form under native conditions. Previous studies have expressed TMV CP and shown that under physiological conditions (neutral pH) these purified CPs form only lower order assemblies that include small aggregates and disks (Bruckman et al, 2011;Dedeo et al, 2010Dedeo et al, , 2011Miller et al, 2007). Recently, a bacterial optimized TMV CP ORF was modified by substituting charge neutralizing amino acids Q and N at position E50 and D77, respectively .…”

Section: Strategies For Epvn Production and Controlled Assemblymentioning

confidence: 99%

“…In addition, genome size constraints dictated by the need for a replication-competent virus genome also limits the types of genetic modifications that can be made to these particles. To circumvent these limitations researchers have for a number of years developed heterologous expression systems for the production of virus-like particles or ePVNs that assemble in the absence of the viral genome (Dedeo et al, 2011;Saunders and Lomonossoff, 2013;Yildiz et al, 2011). ePVNs eliminate the need for infection competent virus while expanding the genetic programmability of the PVNs and decreasing time of production.…”

Section: Empty Plant Virus Nanoparticles (Epvns): Strategies and Applmentioning

confidence: 99%

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Plant virus directed fabrication of nanoscale materials and devices

et al. 2015

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Bottom-up self-assembly methods in which individual molecular components self-organize to form functional nanoscale patterns are of long-standing interest in the field of materials sciences. Such self-assembly processes are the hallmark of biology where complex macromolecules with defined functions assemble from smaller molecular components. In particular, plant virus-derived nanoparticles (PVNs) have drawn considerable attention for their unique self-assembly architectures and functionalities that can be harnessed to produce new materials for industrial and biomedical applications. In particular, PVNs provide simple systems to model and assemble nanoscale particles of uniform size and shape that can be modified through molecularly defined chemical and genetic alterations. Furthermore, PVNs bring the added potential to "farm" such bio-nanomaterials on an industrial scale, providing a renewable and environmentally sustainable means for the production of nano-materials. This review outlines the fabrication and application of several PVNs for a range of uses that include energy storage, catalysis, and threat detection.

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Section: Strategies For Epvn Production and Controlled Assemblymentioning

confidence: 97%

Section: Chemical Conjugationmentioning

confidence: 98%

Section: Strategies For Epvn Production and Controlled Assemblymentioning

confidence: 99%

Section: Empty Plant Virus Nanoparticles (Epvns): Strategies and Applmentioning

confidence: 99%

See 2 more Smart Citations

Plant virus directed fabrication of nanoscale materials and devices

et al. 2015

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show abstract

“…Recent reports have shown that both doublestranded short-interfering RNAs (siRNAs) and single-stranded siRNAs (ss-RNAs) could induce mRNA cleavage in vitro and in vivo through an RNAi-based mechanism [4]. To effectively deliver functional RNAs into target tissues and cells, a variety of delivery strategies have been previously explored to improve transfection efficiency and block digestion of oligonucleotides by nucleases, including lentiviruses, cationic lipid vectors, synthetic polymer systems, viral capsids, inorganic nanotubes, locked nucleic acids, and short hybrid gapmers [5][6][7][8][9]. However, these delivery strategies are not only complicated and expensive, but they also result in suboptimal performance, and potential toxicity [10][11][12].…”

Section: Introductionmentioning

confidence: 99%