Virgin olive oil blended polyurethane micro/nanofibers ornamented with copper oxide nanocrystals for biomedical applications

Abstract

Recently, substantial interest has been generated in using electrospun biomimetic nanofibers of hybrids, particularly organic/inorganic, to engineer different tissues. The present work, for the first time, introduced a unique natural and synthetic hybrid micronanofiber wound dressing, composed of virgin olive oil/copper oxide nanocrystals and polyurethane (PU), developed via facile electrospinning. The as-spun organic/inorganic hybrid micronanofibers were characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis, X-ray diffraction, electron probe microanalysis, and transmission electron microscopy. The interaction of cells with scaffold was studied by culturing NIH 3T3 fibroblasts on an as-spun hybrid micronanofibrous mat, and viability, proliferation, and growth were assessed. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results and SEM observation showed that the hybrid micronanofibrous scaffold was noncytotoxic to fibroblast cell culture and was found to benefit cell attachment and proliferation. Hence our results suggest the potential utilization of as-spun micronanoscaffolds for tissue engineering. Copper oxide–olive oil/PU wound dressing may exert its positive beneficial effects at every stage during wound-healing progression, and these micronanofibers may serve diverse biomedical applications, such as tissue regeneration, damaged skin treatment, wound healing applications, etc. Conclusively, the fabricated olive oil–copper oxide/PU micronanofibers combine the benefits of virgin olive oil and copper oxide, and therefore hold great promise for biomedical applications in the near future.

Preparation and antitumor evaluation of self-assembling oleanolic acid-loaded Pluronic P105/D-α-tocopheryl polyethylene glycol succinate mixed micelles for non-small-cell lung cancer treatment

“…The blank DMEM served as control. 26 After 24 h, images were taken using an OLYMPUS digital camera with an inverted microscope.…”

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

“…For example, blending PU with propolis improved the mechanical strength and hydrophilicity of the nanofibrous membrane, its cytocompatibility with fibroblasts and its antibacterial activity [38]. Blending PU with virgin olive oil endowed the nanofibrous meshes with photoprotective and antioxidant properties [19]. Dextran in composite PU/dextran fibers had angiogenic activity, and also served as a carrier for incorporation of β-estradiol, which accelerated healing of acute cutaneous wounds by its potent anti-inflammatory activity [7].…”

“…Another promising nanofibrous membrane applicable for wound dressing was prepared from electrospun PU, treated by plasma and subsequent spraying with chitosan solution containing an inclusion complex of β-cyclodextrin encapsulating berberine, i.e., an isoquinoline alkaloid with antimicrobial and anti-inflammatory activity [39]. Other antimicrobial substances incorporated into PU-based nanofibers included silver nanoparticles [20,40], copper oxide nanocrystals [19] and antibiotics, such as silver-sulfadiazine [41] and amoxicillin [37]. In general, all these scaffolds showed none or low toxicity towards human HaCaT keratinocytes [20] or fibroblasts [19,40], and no adverse reactions when implanted into laboratory animals in vivo [37,41].…”

“…Other antimicrobial substances incorporated into PU-based nanofibers included silver nanoparticles [20,40], copper oxide nanocrystals [19] and antibiotics, such as silver-sulfadiazine [41] and amoxicillin [37]. In general, all these scaffolds showed none or low toxicity towards human HaCaT keratinocytes [20] or fibroblasts [19,40], and no adverse reactions when implanted into laboratory animals in vivo [37,41]. In addition, copper oxide is known by its angiogenic activity [19].…”

“…In general, all these scaffolds showed none or low toxicity towards human HaCaT keratinocytes [20] or fibroblasts [19,40], and no adverse reactions when implanted into laboratory animals in vivo [37,41]. In addition, copper oxide is known by its angiogenic activity [19]. Nanofibrous meshes created by electrospinning from blends of PU with various concentrations of hydroxypropyl cellulose were also tested for transdermal drug delivery using donepezil hydrochloride, i.e., a drug used for treatment of Alzheimer disease [42].…”