Information and Communication Technologies are driving the improvement of industrial processes. According to the Industry 4.0 (I4.0) paradigm, digital systems provide real-time information to humans and machines, increasing flexibility and efficiency in production environments. Based on the I4.0 Design Principles concept, Virtual Assistants can play a vital role in processing production data and offer contextualized and real-time information to the workers in the production environment. This systematic review paper explored Virtual Assistant applications in the context of I4.0, discussing the Technical Assistance Design Principle and identifying the characteristics, services, and limitations regarding Virtual Assistant use in the production environments. The results showed that Virtual Assistants offer Physical and Virtual Assistance. Virtual Assistance provides real-time contextualized information mainly for support, while Physical Assistance is oriented toward task execution. Regarding services, the applications include integration with legacy systems and static information treatment. The limitations of the applications incorporate concerns about information security and adapting to noisy and unstable environments. It is possible to assume that the terminology of Virtual Assistants is not standardized and is mentioned as chatbots, robots, and others. Besides the worthy insights of this research, the small number of resulting papers did not allow for generalizations. Future research should focus on broadening the search scope to provide more-significant conclusions and research possibilities with new AI models and services, including the emergent Industry 5.0 concept.