Virulence Profile and Antimicrobial Resistance of Escherichia coli from Flies Captured from Agricultural Environment

Yun, Bohyun; Jang, Youn Jung; Kim, Yeon Rok; Kim, Hwang-Yong; Kim, Won-Il; Han, Sang-Hyun; Kim, Se-Ri; Ryu, Jae-Gee; Kim, Hyun Ju

doi:10.13103/jfhs.2017.32.2.147

Cited by 1 publication

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“…Each diluted bacterial suspension was placed in the cassette of the VITEK apparatus along with and AST-N211 card (bioMerieux, France) coated with 17 antibiotics: ampicillin, amoxicillin/sulbactam, piperacillin/tazobactam, cefazolin, cefoxitin, cefotaxime, ceftazidime, cefepime, aztreonam, ertapenem, meropenem, amikacin, gentamicin, levofloxacin, tetracycline, and trimethoprim/sulfamethoxazole. Each cassette was put into the apparatus for analysis as detailed in the VITEK manual [31]. The results were determined according to the National Committee for Clinical and Laboratory Standards guideline [31].…”

Section: Characterization Of E Coli Isolated From Watermentioning

confidence: 99%

“…Each cassette was put into the apparatus for analysis as detailed in the VITEK manual [31]. The results were determined according to the National Committee for Clinical and Laboratory Standards guideline [31]. E. coli ATCC 25922 and E. coli ATCC 35218 (American Type Culture Collection, Manassas, VA, USA) were used as the standards for antibiotic resistance.…”

Section: Characterization Of E Coli Isolated From Watermentioning

confidence: 99%

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Microbiological quality and characteristics of isolated Escherichia coli in irrigation water used in Napa cabbage cultivation

et al. 2018

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To ensure the safety of Kimchi, the safety of Napa cabbage is the most important. Contaminated irrigation water can be a major cause of pathogens during growth of Napa cabbage. The purpose of this study was to investigate the microbial quality of irrigation water used in the cultivation of Napa cabbage. A total of 111 samples including surface water (n = 75) and groundwater (n = 36) collected from four different regions in Korea where Napa cabbage is intensively cultivated were analyzed for a fecal indicator (Escherichia coli) Moreover, 164 E. coli isolates from irrigation water were investigated for pathogenic characteristics including antibiotic resistance, pathogenic genes, serotype, and toxicity using Caenorhabditis elegans. E. coli was detected in 96% of surface water samples and 25% of groundwater samples. The level of E. coli in surface water (0.2-3.2 log MPN/100 mL) was higher than that in groundwater (0-2.0 log MPN/100 mL). When the 164 E. coli isolates were investigated concerning antibiotic resistance, resistance rates were 11.0%, 2.4%, 3.0%, 1.8%, 2.4%, 4.3%, and 3.0% for ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, gentamicin, levofloxacin, and trimethoprim/sulfamethoxazole, respectively. In addition, 10 (6.1%) of the isolates were positive for the eaeA gene, indicative of enteropathogenic E. coli. Eight of these 10 isolates were obtained from the surface water of the mountainous region II and were toxic to C. elegans. The results indicate the need to manage the microbial risk of irrigation water to enhance the safety of cultivated Napa cabbage.

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Section: Characterization Of E Coli Isolated From Watermentioning

confidence: 99%

Section: Characterization Of E Coli Isolated From Watermentioning

confidence: 99%