Virus‐induced gene silencing in tomato

Liu, Yule; Schiff, Michael; Dinesh-Kumar, S. P.

doi:10.1046/j.1365-313x.2002.01394.x

Cited by 1,385 publications

(1,372 citation statements)

References 39 publications

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“…1), indicating that pTRV infection efficiency was close to, or even at, 100%. As expected 27 , the occurrence of photobleached spots within these plants was patchy ( Supplementary Fig. 1a).…”

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confidence: 81%

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A single locus confers tolerance to continuous light and allows substantial yield increase in tomato

et al. 2014

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An important constraint for plant biomass production is the natural day length. Artificial light allows for longer photoperiods, but tomato plants develop a detrimental leaf injury when grown under continuous light-a still poorly understood phenomenon discovered in the 1920s. Here, we report a dominant locus on chromosome 7 of wild tomato species that confers continuous light tolerance. Genetic evidence, RNAseq data, silencing experiments and sequence analysis all point to the type III light harvesting chlorophyll a/b binding protein 13 (CAB-13) gene as a major factor responsible for the tolerance. In Arabidopsis thaliana, this protein is thought to have a regulatory role balancing light harvesting by photosystems I and II. Introgressing the tolerance into modern tomato hybrid lines, results in up to 20% yield increase, showing that limitations for crop productivity, caused by the adaptation of plants to the terrestrial 24-h day/night cycle, can be overcome

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confidence: 81%

“…The parameter F v /F m represents the maximum quantum efficiency of PSII; therefore, the lower the F v /F m value, the higher the injury is. The tomato CAB-13 gene consists of three exons; a unique region from exon 3 was cloned into the VIGS vector pTRV 27,28 (Fig. 2a).…”

mentioning

confidence: 99%

A single locus confers tolerance to continuous light and allows substantial yield increase in tomato

et al. 2014

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“…TRV-based VIGS (Liu et al, 2002) was used to investigate CaHSP70a loss of function in pepper plants. The nonconserved 39 untranslated regions of CaHSP70a and CaSGT1 cDNAs were digested with EcoRI, and the resulting fragment was inserted into pTRV2.…”

Section: Vigsmentioning

confidence: 99%

Pepper Heat Shock Protein 70a Interacts with the Type III Effector AvrBsT and Triggers Plant Cell Death and Immunity

Kim

Hwang

2014

Plant Physiology

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Heat shock proteins (HSPs) function as molecular chaperones and are essential for the maintenance and/or restoration of protein homeostasis. The genus Xanthomonas type III effector protein AvrBsT induces hypersensitive cell death in pepper (Capsicum annuum). Here, we report the identification of the pepper CaHSP70a as an AvrBsT-interacting protein. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirm the specific interaction between CaHSP70a and AvrBsT in planta. The CaHSP70a peptide-binding domain is essential for its interaction with AvrBsT. Heat stress (37°C) and Xanthomonas campestris pv vesicatoria (Xcv) infection distinctly induce CaHSP70a in pepper leaves. Cytoplasmic CaHSP70a proteins significantly accumulate in pepper leaves to induce the hypersensitive cell death response by Xcv (avrBsT) infection. Transient CaHSP70a overexpression induces hypersensitive cell death under heat stress, which is accompanied by strong induction of defense-and cell death-related genes. The CaHSP70a peptide-binding domain and ATPase-binding domain are required to trigger cell death under heat stress. Transient coexpression of CaHSP70a and avrBsT leads to cytoplasmic localization of the CaHSP70a-AvrBsT complex and significantly enhances avrBsT-triggered cell death in Nicotiana benthamiana. CaHSP70a silencing in pepper enhances Xcv growth but disrupts the reactive oxygen species burst and cell death response during Xcv infection. Expression of some defense marker genes is significantly reduced in CaHSP70a-silenced leaves, with lower levels of the defense hormones salicylic acid and jasmonic acid. Together, these results suggest that CaHSP70a interacts with the type III effector AvrBsT and is required for cell death and immunity in plants.

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“…The forward primer cxz141a (5¢-cg gaattc ATG CAC CAC CAC CAC CAC CAC ATG GCC GGG GGA TAC TTC AC-3¢) in which an EcoRI restriction site (underlined) and a tag sequence encoding six consecutive histidine residues (underlined and capitalized) were introduced at the 5¢, and the reverse primer cxz142a (5¢-gc ggatcc CTA CTT CCC AAC CAA CCC TCG-3¢) in which a BamHI restriction site (underlined) was introduced at the 5 prime, were used to amplified the chimeric sequence carrying the His-tag and the CfHNNI1 ORF (His-CfHNNI1). This sequence was then inserted through EcoRI and BamHI restriction sites into the TRV vector pYL156, frequently used for virus-induced gene silencing analysis (Liu et al 2002), to release the expression construct pYL156::His-CfHNNI1. A control expression construct pYL156::His-Avr4, which carries the ORF of C. fulvum avirulence gene Avr4, was made in the same way with the primers cxz52a (5¢-cg ggatcc ATG CAC CAC CAC CAC CAC CAC ATG CAC TAC ACA ACC CTC CT-3¢) and cxz53a (5¢-gc ggatcc TCA ATA GCC AGG ATG TCC AAC-3¢).…”

Section: Constructs For Cfhnni1 Transient Expression In Plant Speciesmentioning

confidence: 99%

Cladosporium fulvum CfHNNI1 induces hypersensitive necrosis, defence gene expression and disease resistance in both host and nonhost plants

Cai

Zhou

et al. 2007

Plant Mol Biol

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Nonhost resistance as a durable and broadspectrum defence strategy is of great potential for agricultural applications. We have previously isolated a cDNA showing homology with genes encoding bZIP transcription factors from tomato leaf mould pathogen Cladosporium fulvum. Upon expression, the cDNA results in necrosis in C. fulvum host tomato and nonhost tobacco plants and is thus named CfHNNI1 (for C. fulvum host and nonhost plant necrosis inducer 1). In the present study we report the induction of necrosis in a variety of nonhost plant species belonging to three families by the transient in planta expression of CfHNNI1 using virus-based vectors. Additionally, transient expression of CfHNNI1 also induced expression of the HR marker gene LeHSR203 and greatly reduced the accumulation of recombinant Potato virus X. Stable CfHNNI1 transgenic tobacco plants were generated in which the expression of CfHNNI1 is under the control of the pathogeninducible hsr203J promoter. When infected with the oomycetes pathogen Phytophthora parasitica var. nicotianae, these transgenic plants manifested enhanced expression of CfHNNI1 and subsequent accumulation of CfHNNI1 protein, resulting in high expression of the HSR203J and PR genes, and strong resistance to the pathogen. The CfHNNI1 transgenic plants also exhibited induced resistance to Pseudomonas syringae pv. tabaci and Tobacco mosaic virus. Furthermore, CfHNNI1 was highly expressed and the protein was translocated into plant cells during the incompatible interactions between C. fulvum and host and nonhost plants. Our results demonstrate that CfHNNI1 is a potential general elicitor of hypersensitive response and nonhost resistance.

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Virus‐induced gene silencing in tomato

Cited by 1,385 publications

References 39 publications

A single locus confers tolerance to continuous light and allows substantial yield increase in tomato

A single locus confers tolerance to continuous light and allows substantial yield increase in tomato

Pepper Heat Shock Protein 70a Interacts with the Type III Effector AvrBsT and Triggers Plant Cell Death and Immunity

Cladosporium fulvum CfHNNI1 induces hypersensitive necrosis, defence gene expression and disease resistance in both host and nonhost plants

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