Virus Induction of Osteosarcomas in Mice

Finkel, Miriam P.; Biskis, Birute O.; Jinkins, Patricia B.

doi:10.1126/science.151.3711.698

Cited by 286 publications

(78 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2 The viral fos oncogene (v fos) causes a very specific osteogenic neoplasm in mice (Finkel et al 1966). It seems that v fos transforms a pre-bone cell that retains the capacity for both osteodi and chondroid differentiation.…”

Section: Resultsmentioning

confidence: 99%

Fos and Jun. Oncogenic Transcription Factors.

Curran

1992

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Fos and Jun. Oncogenic Transcription Factors.

Curran

1992

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

“…The c-fos proto-oncogene is the cellular homolog of the transforming gene of FBJ osteosarcoma virus (6,9,12) and encodes a nuclear protein that is thought to be involved in normal cellular growth and differentiation (7,35). In a number of different cell types, transcription of c-fos is rapidly and transiently activated by growth factors (1,8,19,22,25,26,31,32).…”

mentioning

confidence: 99%

c-fos sequence necessary for basal expression and induction by epidermal growth factor, 12-O-tetradecanoyl phorbol-13-acetate and the calcium ionophore.

Fisch¹,

Prywes²,

Roeder³

1987

Mol. Cell. Biol.

270

181

View full text Add to dashboard Cite

We have investigated the sequence requirements for induction of the human c-fos gene by epidermal growth factor (EGF), 12-0-tetradecanoyl phorbol-13-acetate (TPA), and the calcium ionophore A23187 by transfecting c-fos promoter mutants into HeLa and A431 cells. Induction by both EGF and TPA in HeLa cells required the presence of the c-fos enhancer located at -317 to -298 relative to the mRNA cap site. A23187, however, did not induce expression of the transfected gene, even though it strongly induced expression of the endogenous gene, suggesting that it has different requirements for induction than do EGF and TPA. We have also investigated the role of promoter sequences downstream of the enhancer in general expression and induction of c-fos. A sequence between -97 and -76, which includes an 8-base-pair perfect direct repeat, was needed for efficient general expression but not for induction of the gene. A factor in nuclear extracts that bound specifically to this sequence was detected by a gel mobility shift assay. A 7-base-pair sequence, located between -63 and -57 relative to the mRNA cap site and previously shown to be important for general expression of mouse c-fos, was also important for general expression of the human gene. In addition, this element was important for inducibility by EGF and TPA, since induction was significantly reduced when internal deletion mutants that retained the enhancer but lacked the -63 to -57 sequence element were analyzed in transfection assays.

show abstract

“…Several methods to induce bone tumors were undertaken ; zink beryllium (12), radioactive compounds (11 , 19, 24, 48), chemical carcinogens (19,22,46), and X-ray irradiation (9,40) . Recently a role played by a virus in the oncogenic process of osteosarcoma has been reported (10). The original tumor of Gardner mouse osteosarcoma 519 was found in a CS 7BL~ x CBA Fl hybrid mouse which had received 175R of X-rays.…”

Section: Discussionmentioning

confidence: 99%