Virus integration and genome influence in approaches to stem cell based therapy for andro-urology

Li, Longkun; Zhang, Deying; Damaser, Margot S.; Zhang, Yuanyuan

doi:10.1016/j.addr.2014.10.012

Cited by 13 publications

(6 citation statements)

References 88 publications

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“… 64 , 65 The enhancement of cellular retention, cellular differentiation, and cellular paracrine effects after transplantation are the mechanisms by which the abovementioned engineered stem cells exert powerful effects. 66 The research and application of novel biomaterials have brought new opportunities for the development of SCT in ED. The retention time of stem cells in the corpus cavernosum is extended when combined with materials such as hydrogels, scaffolds, and magnetic nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Bibliometric and Visualization Analysis of Stem Cell Therapy for Erectile Dysfunction

Sun,

Liu,

Yuan

et al. 2024

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Purpose As a common male disease, erectile dysfunction (ED) seriously affects the physical and mental health of patients. In recent years, studies have continued to point out the great potential of stem cell therapy (SCT) in the treatment of ED. The purpose of this study is to comprehensively analyze the research of SCT for ED and understand the development trends and research frontiers in this field. Methods Publications regarding SCT and ED were retrieved and collected from the Web of Science Core Collection. CiteSpace and VOSviewer software were then utilized for bibliometric and visualization analysis. Results A total of 524 publications were eventually included in this study. The annual number of publications in this field was increasing year by year. China and the USA were the two most productive countries. Lin GT, Lue TF and Lin CS, and the University of California San Francisco where they worked were the most productive research group and institution, respectively. The journal with the largest number of publications was The Journal of Sexual Medicine, and the following were mostly professional journals of urology and andrology. Diabetes mellitus-induced ED and cavernous nerve injury-related ED were the two most commonly constructed models of ED in studies. Concerning the types of stem cells, mesenchymal stem cells derived from adipose and bone marrow were most frequently used. Moreover, future research would mainly focus on exosomes, tissue engineering technology, extracorporeal shockwave therapy, and clinical translation. Conclusion The research of SCT for ED will receive increasing global attention in the future. Our study provided bibliometric and visualization analysis of published literature, helping researchers understand the global landscape and frontiers in this field. More preclinical and clinical studies should be conducted to more deeply explore the underlying mechanisms of treatment and promote clinical translation.

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Section: Discussionmentioning

confidence: 99%

Bibliometric and Visualization Analysis of Stem Cell Therapy for Erectile Dysfunction

Sun,

Liu,

Yuan

et al. 2024

DDDT

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“…It is showed that combing cell therapy with gene therapy remarkably improve differentiation of implanted MSCs, revascularization, and innervation in genitourinary tissues, especially in the treatment of ED. [24] Bivalacqua. et al [11] rst used the genetic BM-MSCs modi cation technique on treating ED, which using MSCs modi ed with endothelial nitric oxide synthase (eNOS) to reverse age-associated ED.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells Modified With Short Hairpin RNA (shRNA) Constructs Targeting Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) Improve Erectile Dysfunction in old Rats

Pu¹,

Zhang²,

Zhou³

et al. 2020

Preprint

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Background: We have confirmed that injection of vector-based short hairpin RNA (shRNA) constructs targeting insulin-like growth factor binding protein-3 (IGFBP-3) in penis in old rat can improve erectile function. The aim of this study is to further determine the feasibility of bone marrowe derived mesenchymal stem cells (BM-MSCs) genetically modified with shRNA constructs targeting IGFBP-3 on improving erectile function in the aged rat. Methods: 40 old (24 months) male rats were randomized divided into 4 groups: PBS-only, BM-MSC treatment and LV3-shIGFBP-3 engineered BM-MSC treatment group (n=10/group).Ten 5-month-old rats were used as the young group. Four weeks after transplantation, the erectile function was assessed by cavernosal nerve stimulation. The percent of smooth muscle in corpus cavernosum was evaluated by masson’s trichrome staining. IGFBP-3 expression was estimated by Western blot. The cavernous insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) levels were analyzed after transplantation. Apoptosis in corpus cavernosum was measured using TUNEL and the activity of nitric oxide synthase (NOS) and concentration of guanosine 3’, 5’-cyclic-monophosphates (cGMP) in penile tissue were also analyzed. Results: Four weeks after intracavernous injection, BM-MSCs alone, especially LV3-shIGFBP-3 engineered BM-MSCs treatment rats had improvements in erectile function (p<0.05). The percentage of cavernosal smooth muscle was increased in BM-MSCs and LV3-shIGFBP-3 engineered BM-MSCs treatment group. IGFBP-3 protein expression was decreased obviously after injection of LV3-shIGFBP-3 engineered BM-MSCs. In addition, significant lower IGFBP-3 expression was associated with increased IGF-1 expression in LV3-shIGFBP-3 engineered BM-MSCs treatment rats although higher IGF-1 and VEGF concentrations were all determined after transplantation with BM-MSCs alone or BM-MSCs modified with LV3-shIGFBP-3. Lower levels of the apoptosis were also observed in the BM-MSCs and LV3-shIGFBP-3 engineered BM-MSCs treatment group. NOS activities and cGMP concentrations were also significantly increased after transplantation. Conclusions: These findings demonstrate that BM-MSCs alone or genetically modified with LV3-shIGFBP-3 can improve diminished erectile responses in the aged rat partially by increasing the smooth muscle integrity, increasing secretion of IGF-1 and VEGF, inhibiting apoptosis and increasing the NOS activity and cGMP accumulation.

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“…13,20 These genetically engineered MSCs can thereby both improve MSC engraftment and functionality, while also allowing for the targeted delivery of therapeutic gene products that can enhance local tissue healing. 21 Indeed, MSCs can secret a broad profile of active molecules including hematopoietic growth factors, angiogenic growth factors, trophic molecules, immunomodulatory cytokines, and chemokines. The best-characterized GFs and cytokines produced by these cells are compiled in Table 1.…”

Section: The Relationship Between Msc Biology and Gf Secretionmentioning

confidence: 99%

<p>Growth Factor Gene-Modified Mesenchymal Stem Cells in Tissue Regeneration</p>

Nie¹,

Zhang²,

Wang³

2020

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There have been marked changes in the field of stem cell therapeutics in recent years, with many clinical trials having been conducted to date in an effort to treat myriad diseases. Mesenchymal stem cells (MSCs) are the cell type most frequently utilized in stem cell therapeutic and tissue regenerative strategies, and have been used with excellent safety to date. Unfortunately, these MSCs have limited ability to engraft and survive, reducing their clinical utility. MSCs are able to secrete growth factors that can support the regeneration of tissues, and engineering MSCs to express such growth factors can improve their survival, proliferation, differentiation, and tissue reconstructing abilities. As such, it is likely that such genetically modified MSCs may represent the next stage of regenerative therapy. Indeed, increasing volumes of preclinical research suggests that such modified MSCs expressing growth factors can effectively treat many forms of tissue damage. In the present review, we survey recent approaches to producing and utilizing growth factor gene-modified MSCs in the context of tissue repair and discuss its prospects for clinical application.

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Virus integration and genome influence in approaches to stem cell based therapy for andro-urology

Cited by 13 publications

References 88 publications

Bibliometric and Visualization Analysis of Stem Cell Therapy for Erectile Dysfunction

Bibliometric and Visualization Analysis of Stem Cell Therapy for Erectile Dysfunction

Mesenchymal Stem Cells Modified With Short Hairpin RNA (shRNA) Constructs Targeting Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) Improve Erectile Dysfunction in old Rats

<p>Growth Factor Gene-Modified Mesenchymal Stem Cells in Tissue Regeneration</p>

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