Virus removal from semen with a pinched flow fractionation microfluidic chip

Abstract

Nowadays pigs are bred with artificial insemination to reduce costs and transportation. To prevent the spread of diseases, it is important to test semen samples for viruses. Screening techniques applied...

Tuning particle inertial separation in sinusoidal channels by embedding periodic obstacle microstructures

“…The active technologies utilise external sources such as electrical, [12][13][14] magnetic, 15,16 acoustic, 17,18 optical 19,20 and thermal 21,22 fields. In contrast, passive technologies rely on intrinsic channel geometry, fluid rheology or fluid dynamics, including microfilters, 23,24 pinched flow fractionation (PFF), 25,26 deterministic lateral displacement (DLD), 27,28 inertial [29][30][31] and viscoelastic microfluidics. 32,33 In general, active methods have excellent cell manipulation accuracy by adjusting external force fields in real-time, but the throughput is generally low.…”

Recent microfluidic advances in submicron to nanoparticle manipulation and separation

“…Exosomes with cup-shaped morphology of 30–100 nm were collected at outlet 2, whereas aggregates with sizes of 500 to 2000 nm were gathered at outlet 8. Meanwhile, Hamacher et al 233 developed a microfluidic chip based on PFF to separate spermatozoa from virus-spiked semen. With the optimised flow rate ratios (total flow/sample flow) of 24–32 and fluid removal fraction (percentage of flow to waste outlet) of 2.7%, they recovered 86 ± 6% of spermatozoa and removed 84 ± 4% of cowpea chlorotic mottle virus (CCMV).…”

“…Submicron (0.10-1.0 μm) and nanoparticles (1.0-100 nm) such as extracellular vesicles (EVs), 1 bacteria, 2 viruses, 3 metal, 4 carbon, 5 and polymer nanoparticles, 6 applications in disease diagnostics, drug delivery and material synthesis. The capability to manipulate and separate these tiny particles for isolating and enriching a specific population with uniform properties (e.g., size, shape, and charge) is critical for these applications.…”

“…Submicron (0.10–1.0 μm) and nanoparticles (1.0–100 nm) such as extracellular vesicles (EVs), 1 bacteria, 2 viruses, 3 metal, 4 carbon, 5 and polymer nanoparticles, 6 etc. have broad applications in disease diagnostics, drug delivery and material synthesis.…”

Viscoelastic microfluidics for enhanced separation resolution of submicron particles and extracellular vesicles

“…Manipulation, focusing, and sorting of submicron and nanoparticles such as exosomes, 1 extracellular vesicles, 2 viruses, 3 bacteria, 4 metals 5 and polymer nanoparticles 6 are indispensable in disease diagnostics and therapeutics, drug discovery and material synthesis. 7–9 Conventional technologies such as ultracentrifugation, ultrafiltration, size-exclusion chromatography, precipitation and immunoaffinity capture have been developed to manipulate and separate those minuscule particles.…”