Virus tracking technologies and their applications in viral life cycle: research advances and future perspectives

Abstract

Viruses are simple yet highly pathogenic microorganisms that parasitize within cells and pose serious threats to the health, economic development, and social stability of both humans and animals. Therefore, it is crucial to understand the dynamic mechanism of virus infection in hosts. One effective way to achieve this is through virus tracking technology, which utilizes fluorescence imaging to track the life processes of virus particles in living cells in real-time, providing a comprehensively and detailed spatiotemporal dynamic process and mechanism of virus infection. This paper provides a broad overview of virus tracking technology, including the selection of fluorescent labels and virus labeling components, the development of imaging microscopes, and its applications in various virus studies. Additionally, we discuss the possibilities and challenges of its future development, offering theoretical guidance and technical support for effective prevention and control of the viral disease outbreaks and epidemics.

Development of Stable Infectious cDNA Clones of Tomato Black Ring Virus Tagged with Green Fluorescent Protein

“…The successful constitution was conducted in 2017, when the full length of the cDNA of both RNAs was cloned into a pJL89 binary vector and infiltrated into the plants by agroinoculation. Virus tracking technologies are crucial for studying the virus replication process and understanding the mechanisms involved in the virus life cycle, including virus adsorption and internalization, transportation, genome delivery, assembly, and egress [ 39 ]. Therefore, the goal of this study was to develop stable TBRV-based constructs suitable for expressing a reporter protein, GFP, in plants.…”

Exploring the Replication Mechanisms of DNA and RNA Viruses

“…In conclusion, delving into the intricacies of the virus replication cycle is vital for grasping their infectious tactics, formulating effective antiviral interventions, pioneering technologies rooted in the virus life cycle, and expanding our comprehension of these extraordinary entities to safeguard humanity from the perils of viral infections [7,11].…”