VisEgg: a robust phenotyping tool to assess rainbow trout egg features and viability

Cardona, Emilie; Bugeon, Jérôme; Segret, Emilien; Bobe, Julien

doi:10.1007/s10695-020-00844-2

Cited by 7 publications

(5 citation statements)

References 19 publications

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“…Inversely, when eggs are of bad quality, the vitelline membrane is weakened and the water pressure is enough to break it. In this case, eggs turns white during hydration [35]. The higher fry percent survival after resorption also confirmed higher egg quality from females fed the plant-based diet.…”

Section: Validation Of Dha-rich Microalgae As Supplementation In a Pl...supporting

confidence: 57%

“…Stripped unfertilized eggs (approximatively 400 eggs, about 25 to 30 g) were placed in 150 mL containers and water was added to hydrate the eggs. After 24 h of hydration, a picture of the sampled eggs was obtained using the VisEGG shooting system consisting of a light tablet and digital SLR camera (canon EOS 1000D, resolution: 10.1 M pixels) equipment, as previously described [35]. Pictures were analyzed with Visilog 7.3 software (Thermo Scientific) allowing for automatic measurements to be made (number and size of the eggs and white egg percentage).…”

Section: Automatic Egg Size Number and Integrity Analysismentioning

confidence: 99%

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Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

Cardona

Segret

Cachelou³

et al. 2022

J Animal Sci Biotechnol

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Background The broodstock diet, and in particular the lipid and fatty acid composition of the diet, is known to play a key role in reproductive efficiency and survival of the progeny in fish. A major problem when replacing both fish meal and fish oil by plant sources is the lack of n-3 long chain polyunsaturated fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). To address this problem, we studied the effect of the plant-based diet supplemented with Schizochytrium sp. microalgae, source of DHA, compared to a conventional commercial diet rich in fish meal and fish oil on reproductive performance and egg quality and the consequences on progeny, in female rainbow trout broodstock. Results The results demonstrated that DHA-rich microalgae supplementation in a plant-based diet allowed for the maintenance of reproductive performance and egg quality comparable to a conventional commercial feed rich in fish meal and fish oil and led to an increased significant fry survival after resorption. Moreover, when females were fed a plant-based diet supplemented with micro-algae, the 4-month-old progenies showed a significant higher growth when they were challenged with a similar diet as broodstock during 1 month. We provide evidence for metabolic programming in which the maternal dietary induced significant protracted effects on lipid metabolism of progeny. Conclusions The present study demonstrates that supplementation of a plant-based diet with DHA-rich microalgae can be an effective alternative to fish meal and fish oil in rainbow trout broodstock aquafeed.

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Section: Validation Of Dha-rich Microalgae As Supplementation In a Pl...supporting

confidence: 57%

Section: Automatic Egg Size Number and Integrity Analysismentioning

confidence: 99%

Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

Cardona

Segret

Cachelou³

et al. 2022

J Animal Sci Biotechnol

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“…To do so, the eggs of each fertilization test were transferred into a petri dish and a picture was taken. The total number of eggs was counted with the macro VisEgg 59 on Fiji-ImageJ while the number of fertilized oocytes (embryos with developed eyes) was manually counted with the Fiji-ImageJ counter. Fertilization percentage was calculated by the following formula: (number of fertilized eggs/total number of eggs × 100).…”

Section: Methodsmentioning

confidence: 99%

Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa

El Kamouh,

Brionne,

Sayyari

et al. 2023

Sci Rep

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Spermatozoa are the cells that are most commonly used for cryopreservation of valuable genetic resources in aquaculture. It is known that fish spermatozoa transmit to the embryo not only their genetic but also their epigenetic profile, especially DNA methylation. Therefore, any alteration of the DNA methylation profile in spermatozoa induces the risk of transmitting epigenetic alterations to the offspring. The aim of this study was to assess the effect of cryopreservation on DNA methylation in rainbow trout spermatozoa. To trigger variable cellular response after freezing–thawing, spermatozoa from mature males were cryopreserved with dimethyl sulfoxide, methanol or glycerol as cryoprotectant. We observed that dimethyl sulfoxide was the best to preserve thawed spermatozoa functions. Methanol only slightly preserved all the cellular parameters, while glycerol failed to protect motility and fertilization ability. The consequences on DNA methylation were assessed using Reduced Representation Bisulfite Sequencing (RRBS). Sperm cryopreservation did not thoroughly impact DNA methylation, although 335–564 differentially methylated cytosines were characterized depending on the cryoprotectant. Very few of them were shared between cryoprotectants, and no correlation with the extent of cellular damage was found. Our study showed that DNA methylation was only slightly altered after sperm cryopreservation, and this may render further analysis of the risk for the progeny very challenging.

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“…Ovulation was monitored three time a week. On the control site, a total of 32 fish were used including 18 for egg quality assessment using the VisEgg system (Cardona et al 2020) (4 of them being also used for the oocyte hydration study and 11 of them being also used for blood sampling) and 14 for the molecular analysis. On the LSW site, a total of 46 fish were used including 30 for egg quality assessment using the VisEgg system (4 of them being also used for the oocyte hydration study and 9 of them being also used for blood sampling) and 16 for the molecular analysis.…”

Section: Experimental Design and Water Characterizationmentioning

confidence: 99%

“…Oocyte viability evaluation was systematically performed using the VisEgg protocol when ovulation occurred. This recently developed tool allows a robust assessment of different egg features including egg viability (Cardona et al, 2020). The process uses a standardized image-base batch analysis method of 24h-hydrated unfertilized eggs and an image processing algorithm.…”

Section: Egg Quality Assessment Using Viseggmentioning

confidence: 99%

Effect of a low water concentration in chloride, sodium and potassium on oocyte maturation, oocyte hydration, ovulation and egg quality in rainbow trout

Segret

Cardona

Skiba‐Cassy

et al. 2021

Preprint

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Water salinity is an important environmental factor known to have detrimental effects on salmonid reproduction, mostly when migrating female broodfish are held in sea water. In contrast, data obtained in freshwater are scarce and the impact of low water salinity during reproduction in freshwater is currently unknown. For this reason, and because ion and water fluxes are critical for the final steps of the female gamete formation, including oocyte hydration and ovulation, the aim of the present study was to investigate the impact of low salinity water on final oocyte maturation, ovulation and, ultimately, on egg quality, using rainbow trout as a physiological model and relevant aquaculture species. Fish from the same commercial strain were raised throughout their lifecycle either in a site characterized by low concentrations of Na+, K+, and Cl- ions in the water or in a closely located control site exhibiting standard salinity levels. Egg quality and duration of final oocyte maturation were investigated using innovative phenotyping tools such as automatic assessment of egg viability using the VisEgg system and non-invasive echograph-based monitoring of final oocyte maturation duration, respectively. Oocyte hydration during final oocyte maturation and after ovulation was also investigated. Finally, molecular phenotyping was performed using real-time PCR-based monitoring of several key players of final oocyte maturation and ovulation associated with ion and water transport, inflammation, proteolytic activity, and coagulation. Oocyte hydration and gene expression data were analyzed in the light of the duration of final oocyte maturation. Here we show that low water salinity negatively influences final oocyte maturation, ovulation and, ultimately, egg quality. Low water salinity triggers delayed ovulation and lower oocyte viability. When investigating the impact of low water salinity on final oocyte maturation duration, individuals presenting the most severe phenotypes exhibited impaired oocyte hydration and abnormally reduced gene expression levels of several key players of the ovulatory process. While the under expression of water (e.g., aquaporins) and ion (e.g., solute carriers) transporters is consistent with impaired oocyte hydration, our observations also indicate that the entire ovulatory gene expression program is disrupted. Our results raise the question of the mechanisms underlying the negative influence of low salinity water on the dynamics of the preovulatory phase, on the control of the oocyte homeostasis, including hydration, and on the overall success of the maturation-ovulation process.

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VisEgg: a robust phenotyping tool to assess rainbow trout egg features and viability

Cited by 7 publications

References 19 publications

Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa

Effect of a low water concentration in chloride, sodium and potassium on oocyte maturation, oocyte hydration, ovulation and egg quality in rainbow trout

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