Visible and UVA light as a potential means of preventing Escherichia coli biofilm formation in urine and on materials used in urethral catheters

Vollmerhausen, Tara L.; Conneely, A.; Bennett, Clare L.; Wagner, Victoria E.; Victor, Jospeh; O’Byrne, Conor

doi:10.1016/j.jphotobiol.2017.04.018

Cited by 34 publications

(31 citation statements)

References 46 publications

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“…In addition, at higher microbial inoculum oxygen might be consumed faster than it is re-supplied by diffusion from the surface of the initial suspension (Hessling et al, 2017; Vollmerhausen et al, 2017). Bumah et al investigated the effect of bacterial inoculum on the efficacy of aBL at 405 and 470 nm (Bumah et al, 2015a).…”

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

“…In a similar study, Vollmerhausen et al determined the efficacy of 420-nm aBL for inactivation of uropathogenic E coli in suspensions of urine mucin media at varying initial bacterial inocula from 10 3 to 10 7 CFU/mL (Vollmerhausen et al, 2017). At the inoculum of 10 3 CFU/mL, aBL reduced E. coli to below the detection level after an aBL exposure of 86.4 J/cm 2 (6 h irradiation).…”

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

“…Vollmerhausen et al employed 420-nm aBL to treat uropathogenic E. coli attached to the silicone matrix of a urinary catheter (Vollmerhausen et al, 2017). First, the investigators showed that aBL was able to prevent E. coli biofilm formation by measuring bacterial adherence to the silicone surfaces.…”

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

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Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

227

192

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As an innovative non-antibiotic approach, antimicrobial blue light in the spectrum of 400–470 nm has demonstrated its intrinsic antimicrobial properties resulting from the presence of endogenous photosensitizing chromophores in pathogenic microbes and, subsequently, its promise as a counteracter of antibiotic resistance. Since we published our last review of antimicrobial blue light in 2012, there have been a substantial number of new studies reported in this area. Here we provide an updated overview of the findings from the new studies over the past 5 years, including the efficacy of antimicrobial blue light inactivation of different microbes, its mechanism of action, synergism of antimicrobial blue light with other angents, its effect on host cells and tissues, the potential development of resistance to antimicrobial blue light by microbes, and a novel interstitial delivery approach of antimicrobial blue light. The potential new applications of antimicrobial blue light are also discussed.

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Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

227

192

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“…The mechanism of blue light inactivation primarily involves interaction between photons and oxygen, which leads to production of reactive oxygen species (ROS), as suggested by recent studies that involved blue irradiation of other bacteria . ROS detection and measurement in H. pylori were carried out using Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit *Deep Red Fluorescence* (AAT Bioquest®, Inc., Mercury Drive, Sunnyvale, CA).…”

Section: Methodsmentioning

confidence: 99%

“…,26 ROS detection and measurement in H. pylori were carried out using Cell Meter ™ Fluorimetric Intracellular Total ROS Activity Assay Kit *Deep Red Fluorescence* (AAT Bioquest®, Inc., Mercury Drive, Sunnyvale, CA). Five milliliter of Type A strain H. pylori was pre-cultured overnight, followed by centrifugation at 7000 rpm for 2 min.…”

mentioning

confidence: 99%

Anti‐proliferation effect of blue light‐emitting diodes against antibiotic‐resistant Helicobacter pylori

Hiratsuka

Etoh

et al. 2018

J of Gastro and Hepatol

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Background and Aim Infection by Helicobacter pylori is implicated in a wide range of upper gastrointestinal diseases. Owing to the rapid emergence of antibiotic‐resistant strains of H. pylori, the development of novel treatment modalities for antibiotic‐resistant H. pylori infection is a key priority. Blue light‐emitting diodes (LED) may represent a unique option owing to their antimicrobial effect. In this study, we aimed to evaluate the anti‐proliferative effect of blue LED against antibiotic‐resistant H. pylori. Methods Ten antibiotic‐resistant strains and one sensitive H. pylori strain were used in this study. After irradiation by blue LED along time course, the viability of H. pylori was evaluated by enumerating colony forming units. Morphological changes in H. pylori were observed using a scanning electron microscope. Reductase activity was measured as an indicator of bacterial cellular activity. Total reactive oxygen species was monitored using fluorescence intensity and fluorescence microscope imaging. Results After irradiation by blue LED, the numbers of H. pylori in all the strains were significantly reduced compared with control group. The H. pylori exhibited a short rod‐shaped morphology after irradiation; no such change was observed in H. pylori not exposed to blue LED. Re‐irradiation of surviving strain after the initial irradiation also exhibited the same anti‐proliferation effect. After blue LED irradiation, bacterial cellular activity was lower, and total reactive oxygen species production was significantly higher in blue LED group, compared with that in control. Conclusions Blue LED could be a new treatment to eradicate infection with antibiotic‐resistant H. pylori.

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Antimicrobial Blue Light Inactivation of Microbial Isolates in Biofilms

et al. 2019

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Background and Objectives Biofilms cause more than 80% of infections in humans, including more than 90% of all chronic wound infections and are extremely resistant to antimicrobials and the immune system. The situation is exacerbated by the fast spreading of antimicrobial resistance, which has become one of the biggest threats to current public health. There is consequently a critical need for the development of alternative therapeutics. Antimicrobial blue light (aBL) is a light‐based approach that exhibits intrinsic antimicrobial effect without the involvement of exogenous photosensitizers. In this study, we investigated the antimicrobial effect of this non‐antibiotic approach against biofilms formed by microbial isolates of multidrug‐resistant bacteria. Study Design/Materials and Methods Microbial isolates of Acinetobacter baumannii, Candida albicans, Escherichia coli, Enterococcus faecalis, MRSA, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Proteus mirabilis were studied. Biofilms were grown in microtiter plates for 24 or 48 hours or in the CDC biofilm reactor for 48 hours and exposed to aBL at 405 nm (60 mW/cm2, 60 or 30 minutes). The anti‐biofilm activity of aBL was measured by viable counts. Results The biofilms of A. baumannii, N. gonorrhoeae, and P. aeruginosa were the most susceptible to aBL with between 4 and 8 log10 inactivation after 108 J/cm2 (60 mW/cm2, 30 minutes) or 216 J/cm2 (60 mW/cm2, 60 minutes) aBL were delivered in the microplates. On the contrary, the biofilms of C. albicans, E. coli, E. faecalis, and P. mirabilis were the least susceptible to aBL inactivation (−0.30, −0.24, −0.84, and −0.68 log10 inactivation, respectively). The same aBL treatment in biofilms developed in the CDC biofilm reactor, caused −1.68 log10 inactivation in A. baumannii and −1.74 and −1.65 log10 inactivation in two different strains of P. aeruginosa. Conclusions aBL exhibits potential against pathogenic microorganisms and could help with the significant need for new antimicrobials in clinical practice to manage multidrug‐resistant infections. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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Visible and UVA light as a potential means of preventing Escherichia coli biofilm formation in urine and on materials used in urethral catheters

Cited by 34 publications

References 46 publications

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Anti‐proliferation effect of blue light‐emitting diodes against antibiotic‐resistant Helicobacter pylori

Antimicrobial Blue Light Inactivation of Microbial Isolates in Biofilms

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