Visible light-induced crosslinkable gelatin

Son, Tae Il; Sakuragi, Makoto; Takahashi, Sawa; Obuse, Sei; Kang, Jeonghwa; Fujishiro, Masako; Matsushita, Haruo; Gong, Jian-Sheng; Shimizu, Shigeru; Tajima, Yusuke; Yoshida, Yasuhiro; Suzuki, Kazuomi; Yamamoto, Tetsuro; Nakamura, Mariko; Ito, Yoshihiro

doi:10.1016/j.actbio.2010.05.018

Cited by 67 publications

(63 citation statements)

References 30 publications

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“…The approximate composition of aromatic amino acids in porcine gelatin is 2%, which is similar to the estimate for furan groups in gelatin-FA (2.9%) based on the peak area in 1 H-NMR spectra. To compare gelatin-FA with our previously reported gelatin-FI13, 10% aqueous solutions of gelatin-FA and gelatin-FI were mixed with RB (0.5%) on a plate and illuminated for 10, 30, 60, and 180 s by visible light. The plate was washed to remove uncross-linked solutions and the gelation rate was observed.…”

Section: Resultsmentioning

confidence: 99%

“…We used BMP4 and CBD-BMP4 as growth factors. CBD-BMP4 exhibited stronger and more stable collagen binding activity than did wild-type BMP41318. Because gelatin is a hydrolyzed form of collagen, we hypothesized that CBD-BMP4 may be better retained in gelatin-FA hydrogels than native BMP4.…”

Section: Resultsmentioning

confidence: 99%

“…We have previously developed a new type of gelatin by incorporating furfuryl isocyanate (gelatin-FI), which was photo-cross-linked with Rose Bengal (RB), a food dye. The photo-crosslinkable gelatin-FI was useful as a direct pulp capping material in the dental field13.…”

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confidence: 99%

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A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Mazaki

Sugiyama

Yamane

et al. 2014

Sci Rep

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112

106

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Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37°C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Mazaki

Sugiyama

Yamane

et al. 2014

Sci Rep

Self Cite

112

106

View full text Add to dashboard Cite

show abstract

“…Methacrylation of gelatin has been studied for its cross-linking by photo-initiated radical polymerization [12,13]. Recently, Son et al prepared furan-modified gelatin systems cross-linked by a photo-oxidation process using Rose Bengal as a light sensitizer [14,15]. However, to the best of our knowledge, there is no report on the design of photo-crosslinked furan-based gelatin systems without the use of initiators or catalysts.…”

Section: Introductionmentioning

confidence: 97%

Green chemistry for the cross-linking of photo-sensitive furan modified gelatin

et al. 2015

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“…Considering that porcine gelatin has 11.4 wt% of carboxyl groups of glutamic acid (4.2%) and aspartic acid (7.2%), we concluded that about 14% of these groups were coupled with 3-aminopropylphosphonic acid. We had previously incorporated azidophenyl or furan groups into gelatin in a similar manner, and found that the contents were 84% 44 and near 100%, 45 respectively. The low reactivity with phosphate was considered to be caused by ionic repulsion of the phosphate group.…”

Section: Preparation Of Phosphonated Gelatinmentioning

confidence: 99%

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

Zhou¹,

Park²,

Mao³

et al. 2015

IJN

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Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by 31 P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

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Visible light-induced crosslinkable gelatin

Cited by 67 publications

References 30 publications

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Green chemistry for the cross-linking of photo-sensitive furan modified gelatin

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

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