Visual Detection of Cysteine and Homocysteine

Rusin, Oleksandr; Luce, Nadia N. St.; Agbaria, Rezik A.; Escobedo, Jorge O.; Jiang, Shan; Warner, Isiah M.; Dawan, Fareed; Lian, Kun; Strongin, Robert M.

doi:10.1021/ja036297t

Cited by 504 publications

(245 citation statements)

References 16 publications

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“…13b Highly selective probes for Hcy and Cys are being developed in our lab as potentially useful new detection agents. 22 The selective colorimetric and fluorimetric detection of Cys and Hcy in natural media is reported herein. New HPLC methods are also described.…”

Section: Homocysteine and Cysteine In Human Healthmentioning

confidence: 99%

Detection of Homocysteine and Cysteine

et al. 2005

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At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hcy or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hcy in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.

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Section: Homocysteine and Cysteine In Human Healthmentioning

confidence: 99%

Detection of Homocysteine and Cysteine

et al. 2005

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“…In recent decades, many studies have demonstrated that metabolic disorder of biothiols will lead to extreme damage in human and numerous other animals (Townsend et al, 2003;Reddie and Carroll, 2008;Weerapana et al, 2010). Specifically, a low level of biothiols in vivo may cause slowed growth, liver damage, weight loss, and some other adverse influences (Wang et al, 2005;Duan et al, 2008;Yang et al, 2012), while a high level of biothiols is closely related to Parkinson's, Alzheimer's, and cardiovascular diseases (Heafield et al, 1990;Rusin et al, 2004;Lin et al, 2008;Yang et al, 2011). Thus, a lot of effort has been put into quantifying the lowmolecule-weight biothiols in serum (Ni et al, 2015;Ghasemi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Detection of biological thiols based on a colorimetric method

Sun

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.

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“…Once the quinone is formed, even at low concentrations, it may add up a nucleophilic -SH compound relatively easily. Rusin et al 34 showed that when cysteine or homocysteine reacts with the carbonyl moieties of xanthene dyes, absorptiometric or fluorometric maxima are red-shifted as a result of -SH addition reactions, arising from intramolecular charge transfer (ICT) between electron-rich and electrondeficient groups 35 within the adduct molecule. In the case when phenols are oxidized faster than thiols, the Michael addition of nucleophilic thiols to quinones (i.e.…”

Section: Resultsmentioning

confidence: 99%

A Novel Spectrofluorometric Probe for the Determination of Peroxynitrite Anion Scavenging Activity of Biothiols and Amino Acids

et al. 2016

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In this study, a novel fluorometric method for the determination of peroxynitrite anion (ONOO -) scavenging (PAS) activity of amino acids and biothiols, which can mostly trap peroxynitrite in vivo, is described. This assay is based on the conversion of a gentisic acid probe to its non-fluorescent oxidation products with ONOO -. The attenuation of the fluorescence intensity (FI) of the probe upon peroxynitrite attack is diminished with antioxidants, the difference in FI being related to the PAS activity of the antioxidants. The IC50 (50% inhibitive concentration) values of biothiols, amino acids and tissue homogenates were estimated, in comparison with the reference Pyrogallol Red (PR) bleaching method. PR is the most suitable and frequently used dye to determine PAS activity, but is relatively insensitive. The developed fluorometric assay is highly sensitive to allow determinations of the PAS activity of amino acids.

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Visual Detection of Cysteine and Homocysteine

Cited by 504 publications

References 16 publications

Detection of Homocysteine and Cysteine

Detection of Homocysteine and Cysteine

Detection of biological thiols based on a colorimetric method

A Novel Spectrofluorometric Probe for the Determination of Peroxynitrite Anion Scavenging Activity of Biothiols and Amino Acids

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