Visual detection of glucose based on quantum dots aerogel in microfluidic chips

Hu, Tao; Ye, Yi; Chen, Ke; Long, Feifei; Wen, Shuguang; Zhou, Yunlong; Sun, Dandan; Zhang, Ni

doi:10.1039/c8ay02177e

Cited by 14 publications

(6 citation statements)

References 46 publications

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“…Microfluidics has proven to be a valuable tool for analyzing different types of metabolites, offering fast assay times, portability, low cost, and sample consumption [43]. The list of microfluidics systems employed for metabolite detection includes paperbased devices [115][116][117][118][119][120][121][122][123][124][125][126][127][128], microfluidic droplets [129][130][131][132][133][134], microchannels or microchambers made of polydimethylsiloxane (PDMS) [135][136][137][138][139][140][141][142], or centrifugal microfluidic platforms [143][144][145][146]. Detection methods commonly used for these systems consist of colorimetry [116-118, 120, 121, 123, 131, 133, 143, 147, 148], fluorescence [122,136,145,149], chemiluminescence [145], electrochemistry [115, 119, 127-129, 135, 137, 142], MS [130,134,150,151], or surface plasmo...…”

Section: Metabolite Biomarkersmentioning

confidence: 99%

Section: Metabolite Biomarkersmentioning

confidence: 99%

“…Simple microchannels or microchambers (nl or pl range) can be used as microreactors to carry out biochemical reactions and detect metabolites from serum [135][136][137][138][139][140][141][142]. These microchannels are usually made from PDMS [136,139] and can be sealed over micropatterned electrodes for electrochemical detection of cholesterol [142], for example. But they can also be coupled to external magnetic fields to retain enzyme-functionalized nano-or micro-particles and detect urea [140], creatine [140,141], glucose [135,140,141], and TGL [138] with an electrochemical reaction.…”

Section: Metabolite Biomarkersmentioning

confidence: 99%

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Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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Biomarkers are relevant indicators of the physiological state of an individual.Although biomarkers can be found in diseased tissue and different biofluids, sampling from blood plasma is relatively easy and less invasive. Among the molecular biomarkers that can be found circulating in plasma are proteins, metabolites, nucleic acids, and exosomes. Some of these plasma-circulating biomarkers are now employed for patient stratification in a broad range of diseases with high sensitivity and specificity and are useful in early diagnosis, initial risk assessment, and therapy selection. However, there is a pressing need to develop novel approaches for biomarker analysis that can be translated into clinical or other settings without complex methodologies or instrumentation. Microfluidics has been touted as a promising technology to carry out this task because it offers high-throughput, automation, multiplexed detection, and portability, possibly overcoming the bottleneck that prevent the translation of novel biomarkers to the point-of-care (POC). Here, we provide a review of the microfluidic systems that have been engineered to detect circulating molecular biomarkers in blood plasma. We also review the different microfluidic approaches for plasma enrichment, which are now being integrated with microfluidic-based biomarker analyzers. Such integration should lead to

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Section: Metabolite Biomarkersmentioning

confidence: 99%

Section: Metabolite Biomarkersmentioning

confidence: 99%

Section: Metabolite Biomarkersmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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“…At present, various methods are used in UG detection, including electrochemical devices, − surface-enhanced Raman scattering (SERS), − colorimetric sensors, − and so on. Glucose reacts with enzymes or enzyme-like substances to generate electrons to cause changes in current.…”

Section: Introductionmentioning

confidence: 99%

Portable and Intelligent Urine Glucose Analyzer Based on a CdTe QDs@GOx Aerogel Circular Array Sensor

et al. 2021

ACS Omega

Self Cite

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Diabetes is a public health problem characterized by hyperglycemia, high mortality, and morbidity. A simple, rapid, and sensitive glucose detection method for diabetes screening and health self-management of patients with diabetes is of great significance. Therefore, an attractive urine glucose (UG) analyzer with advantages of fastness, sensitivity, and portability was developed. A cadmium telluride quantum dots (CdTe QDs)@glucose oxidase (GOx) aerogel circular array sensor can emit visible red fluorescence when excited by a 365 nm ultraviolet light source inside the analyzer. When urine samples containing glucose were dropped onto the sensor, glucose was oxidized by GOx to produce hydrogen peroxide (H2O2), which quenched the red fluorescence of CdTe QDs. The fluorescence images of the sensor were obtained using a CCD camera, and the linear relationship between the glucose concentration and the gray value of the fluorescence image was established. The analyzer shows good sensitivity (LOD, 0.12 mM) with a wide linear range of 0.12–26 mM. Based on the linear relation, the software of the analyzer was written in the C++ language, which can automatically give the gray value of the image and the corresponding glucose concentration. The UG analyzer was used for the detection of a large number clinical samples and compared with a variety of UG test papers, which all showed good detection performance. The novel analyzer we proposed has an important significance in the screening of diabetes and the self-management of diabetic patients.

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“…A biosensor can convert different concentrations of glucose into corresponding electrical signals for output, which has advantages of high accuracy, fast analysis, low cost, good repeatability, simple operation and high specificity by comparing with traditional detection methods [10][11][12][13][14][15][16]. With the aid of chips, microfluidics systems and labs-on-a-chip, biosensor technology have developed rapidly and can be divided into electrochemical biosensors and optical biosensors based on the type of signal conversion [17][18][19][20][21][22][23][24][25][26]. Electrochemical biosensors enable the detection of biomarkers by adding specific enzymes on the electrodes, which has many advantages, such as high sensitivity, simple operation and cost-effective [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Reusable, Non-Invasive, and Ultrafast Radio Frequency Biosensor Based on Optimized Integrated Passive Device Fabrication Process for Quantitative Detection of Glucose Levels

Yao

Yue

et al. 2020

Sensors

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The increase in the number of people suffering diabetes has been the driving force behind the development of glucose sensors to overcome the current testing shortcomings. In this work, a reusable, non-invasive and ultrafast radio frequency biosensor based on optimized integrated passive device fabrication process for quantitative detection of glucose level was developed. With the aid of the novel biosensor design with hammer-shaped capacitors for carrying out detection, both the resonance frequency and magnitude of reflection coefficient can be applied to map the different glucose levels. Meanwhile, the corresponding fabrication process was developed, providing an approach for achieving quantitative detection and a structure without metal-insulator-metal type capacitor that realizes low cost and high reliability. To enhance the sensitivity of biosensor, a 3-min dry etching treatment based on chlorine/argon-based plasma was implemented for realizing hydrophilicity of capacitor surface to ensure that the biosensor can be touched rapidly with glucose. Based on above implementation, a non-invasive biosensor having an ultrafast response time of superior to 0.85 s, ultralow LOD of 8.01 mg/dL and excellent reusability verified through five sets of measurements are realized. The proposed approaches are not limited the development of a stable and accurate platform for the detection of glucose levels but also presents a scheme toward the detection of glucose levels in human serum.

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Visual detection of glucose based on quantum dots aerogel in microfluidic chips

Abstract: In this study, we demonstrated a new optical glucose microfluidic sensor based on quantum dots (QDs) aerogels and glucose oxidase (GOx) for rapid, low-cost and quantitative detection of glucose in human urine and serum.

Cited by 14 publications

References 46 publications

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Portable and Intelligent Urine Glucose Analyzer Based on a CdTe QDs@GOx Aerogel Circular Array Sensor

Reusable, Non-Invasive, and Ultrafast Radio Frequency Biosensor Based on Optimized Integrated Passive Device Fabrication Process for Quantitative Detection of Glucose Levels

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