Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles

Abstract

A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs). As a proof-of-principle, novel applications of this method as a versatile biosensor for single-nucleotide polymorphisms (SNPs) and DNA methyltransferase (MTase) were presented.

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

“…With the use of this biosensor to detect pesticides in cabbage, rape, and lettuce samples, relative standard deviations between 0 and 4.51 and a recovery rate between 94% and 105% was observed. These results indicate that the proposed biosensor has remarkable accuracy, precision, and that the process is highly reproducible [275].…”

“…Table 2 shows the types of materials that have been used in the production of enzymatic biosensors based on nanomaterials. (3) Direct detection of paraoxon [141] Plant esterase CS/AuNPs-GNs (4) Detection of organophosphate pesticides [142] Diamine oxidase Ceria nanospheres Determination of putrescine in tiger prawn [143] Xanthine oxidase MNP-PAMAM-PtNP/rGO-CMC (5) Determination of xanthine in fish meat [144] DNA methyltransferase Gold nanoparticles Detection of DNA methyltransferase activity [275] Human monoamine oxidase B MnO 2 modified MWCNTs * Determination of total monoamines [145] Glucose oxidase Tobacco mosaic virus Glucose biosensor [146] Glucose oxidase Carbon ceramic Pore size effect in the amount of immobilized enzyme for manufacturing biosensors [147] Tyrosinase Carbon nanotubes Phenol biosensor [276] Xanthine oxidase poly(l-aspartic acid)/MWCNT Detection of xanthine in food industries [148] Uricase Chi-Gr cry/PB/SPCE (6) Uric acid detection [149]…”

“…As described above, the basic architecture of a biosensor is given by the appropriate combination of a biological portion and a transducer, and the changes that are detected by the transducer are converted into tangible optical, electrical, or thermal signals, which are used to identify the analyte of interest [47]. Thus, depending on the type of electrode and the class of enzyme, these biosensors can applied in several industrial segments, such as in biomedical applications [19,47,[137][138][139], in the monitoring of food, soil, and plantations [275,377,380,381], and in the detection of environmental pollutants [1]. The growing interest of these sectors in this type of device is explained by their high sensitivity and enzyme specificity toward the analytes of interest.…”

Rapid and simple detection of ascorbic acid and alkaline phosphataseviacontrolled generation of silver nanoparticles and selective recognition

“…Semiconductor QDs exhibit unique properties, e.g., width, symmetry, size-tunable emission spectra, broad absorption spectra, long photoluminescence lifetimes, and significant photo-stability, which allow for fascinating applications in sensing, bioimaging, etc. 17,18 Additionally, cation exchange reactions have led to the development of a series of research methods for quantitative assays, 19 which can be used to analyze the characteristics of nucleic acids, 20 proteins, 21 single-nucleotide polymorphisms (SNPs), 22 and other substrates. These reaction conditions are mild, and strong acids and complex steps were not involved.…”

Application of a cation-exchange reaction of CuS nanoparticles and fluorescent copper nanoparticles in a DNA biosensor

“…7 Later, we developed a colorimetric assay for single-nucleotide polymorphisms and DNA methyltransferase based on a cation-exchange reaction of CuS NPs and the click chemistry of azide-and alkyne-functionalized gold nanoparticles. 8 Compared to the acid dissolution of CuS NPs using a strong acid, e.g., HNO 3 , 9 the reaction conditions for the cationexchange of CuS NPs are mild and more suitable for real biological sample detection. However, the operation process is complicated, especially for the click reaction of functionalized gold nanoparticles.…”