2022
DOI: 10.1101/2022.09.13.507877
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Visual evidence for the recruitment of four enzymes with RNase activity to the Bacillus subtilis replication forks

Abstract: Removal of RNA/DNA hybrids for the maturation of Okazaki fragments on the lagging strand, or due to misincorporation of ribonucleotides by DNA polymerases, is essential for all types of cells. In prokaryotic cells such as Escherichia coli, DNA polymerase 1 and RNase HI are supposed to remove RNA from Okazaki fragments, but many bacteria lack HI-type RNases, such as Bacillus subtilis. Here, four proteins have been shown to be able to remove RNA from RNA/DNA hybrids in vitro, but their actual contribution to DNA… Show more

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