Visual neuroscience methods for marmosets: efficient receptive field mapping and head-free eye tracking

Jendritza, Patrick; Klein, Frederike J.; Rohenkohl, Gustavo

doi:10.1101/2020.10.30.361238

Cited by 4 publications

(8 citation statements)

References 98 publications

(87 reference statements)

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“…Reverse correlation analysis was used to locate RF centers across the whole monitor. A detailed account of the RF mapping procedure can be found in Jendritza et al, 2021. As expected from the implantation target position, RFs in area V1 were located in the lower right visual field (Fig.…”

Section: Resultsmentioning

confidence: 93%

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Multi-area recordings and optogenetics in the awake, behaving marmoset

Jendritza

Klein

2021

Preprint

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The common marmoset has emerged as a key primate model in neuroscience. Marmosets are small in size, show great potential as transgenic models and exhibit complex behaviors. These advantages place the marmoset model in the critical gap between rodents and larger primates. Thus, it is necessary to develop technology that enables monitoring and manipulation of the neural circuits underlying the behavior of the marmoset. Here, we present a novel approach to record and optogenetically manipulate neural activity in the awake, behaving marmoset. Our design utilizes a light-weight, 3D printed titanium chamber that can house several high-density silicon probes for semi-chronic recordings, while enabling simultaneous optogenetic stimulation. Surgical procedures are streamlined via custom 3D printed guides and implantation holders. We demonstrate the application of our method by recording multi- and single-unit data from areas V1 and V6 with 192 channels simultaneously, and show for the first time that optogenetic activation of excitatory neurons in area V6 can influence behavior in a detection task. Together, the work presented here will support future studies investigating the neural basis of perception and behavior in the marmoset.

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Section: Resultsmentioning

confidence: 93%

“…All details about the RF mapping procedure have been described previously in Jendritza et al, 2021. RF mapping was performed with stimuli consisting of black wedges and annuli of various orientations and sizes, presented on a gray background for a duration of eight frames (120 Hz monitor refresh rate).…”

Section: Receptive Field Mappingmentioning

confidence: 99%

Multi-area recordings and optogenetics in the awake, behaving marmoset

Jendritza

Klein

2021

Preprint

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“…A detailed account of the RF mapping procedure can be found in ref. 30 . As expected from the implantation target position, RFs in area V1 were located in the lower right visual field (Fig.…”

Section: Simultaneous Recording In Areas V1 and V6mentioning

confidence: 99%

“…All details about the RF mapping procedure have been described previously 30 . RF mapping was performed with stimuli consisting of black wedges and annuli of various orientations and sizes, presented on a gray background for a duration of eight frames (120 Hz monitor refresh rate).…”

Section: Receptive Field Mappingmentioning

confidence: 99%

Multi-area recordings and optogenetics in the awake, behaving marmoset

Jendritza

Klein

2023

Nat Commun

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The common marmoset has emerged as a key model in neuroscience. Marmosets are small in size, show great potential for genetic modification and exhibit complex behaviors. Thus, it is necessary to develop technology that enables monitoring and manipulation of the underlying neural circuits. Here, we describe a novel approach to record and optogenetically manipulate neural activity in awake, behaving marmosets. Our design utilizes a light-weight, 3D printed titanium chamber that can house several high-density silicon probes for semi-chronic recordings, while enabling simultaneous optogenetic stimulation. We demonstrate the application of our method in male marmosets by recording multi- and single-unit data from areas V1 and V6 with 192 channels simultaneously, and show that optogenetic activation of excitatory neurons in area V6 can influence behavior in a detection task. This method may enable future studies to investigate the neural basis of perception and behavior in the marmoset.

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“…Several neurological disease models have also been successfully generated using various methods in marmosets (Eslamboli et al, 2007; Tomioka et al, 2017; Jenner et al, 1984; Fox et al, 2010; Emborg, 2017). Moreover, they can learn to perform a variety of visual and cognitive tasks under head unrestrained conditions (Clarke et al 2011; Jendritza et al 2021; Spinelli et al 2004; Yamazaki et al 2011; Okano 2021) and under head restrained conditions (Mitchell et al 2014; Johnston et al, 2018; Chen et al, 2021; Sedaghat-Nejad et al 2019), and they showed similar to human behaviour when they successfully performed oculomotor tasks.…”

Section: Introductionmentioning

confidence: 99%

Dissecting errors made in response to externally- and internally-driven visual tasks in the common marmosets and humans

Amly

Chen

Onoe

et al. 2021

Preprint

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Various saccadic tasks traditionally used in oculomotor research, including both exogenously-driven and endogenously-driven saccades, have been proposed as clinical diagnostic for human movement disorders. Recently, common marmosets have been proven to be a good primate model for these movement disorders. However, whether similar saccadic measurements can be used for marmosets was not tested. Here, we trained three marmosets on the gap task, an exogenously-driven saccadic task, and the oculomotor delayed response (ODR) task, an endogenously-driven saccadic task. We demonstrated that with long-term training, they were able to learn and switch between the two tasks. The marmosets showed undershooting tendency in the gap and both under- and overshooting tendency in the ODR task when they made saccades to the target. We also categorized the error trails into distractive, impulsive, and visuomotor errors, depending on when a false saccade happened in relation to the task progression and interpreted possible causes of errors based on the saccade response time and the task history. Each error category may indicate specific failures in a particular aspect of cognitive or sensory-motor processes. Finally, and critically, we found that saccades from successful trials exhibited the highest saccade peak velocity and the shortest saccade duration comparing to the same saccade amplitude from error trials. Taken together, we showed the potential of training the same marmosets on conflicting oculomotor tasks simultaneously. We documented the baseline performance and compared successful and error trials in both gap and ODR tasks from the same animals. We propose that analyzing the error trials in sync with successful trials could provide a further understanding of the cognitive and sensorimotor processes of the marmosets, in both healthy and disease conditions.

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Visual neuroscience methods for marmosets: efficient receptive field mapping and head-free eye tracking

Cited by 4 publications

References 98 publications

Multi-area recordings and optogenetics in the awake, behaving marmoset

Multi-area recordings and optogenetics in the awake, behaving marmoset

Multi-area recordings and optogenetics in the awake, behaving marmoset

Dissecting errors made in response to externally- and internally-driven visual tasks in the common marmosets and humans

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