Visualization and Detection of Ciliary Beating Pattern and Frequency in the Upper Airway using Phase Resolved Doppler Optical Coherence Tomography

Jing, Joseph C.; Chen, Jason J.; Chou, Li-Dek; Wong, Brian J. F.; Chen, Zhongping

doi:10.1038/s41598-017-08968-x

Cited by 32 publications

(39 citation statements)

References 49 publications

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“…All analyzed samples showed normal cilial length and configuration as observed in immunofluorescence and ultrastructural analysis. Adult (n = 4) and preterm (n = 3) samples displayed comparable beat pattern and frequencies (11.2 ± 1.6 vs. 10.7 ± 1.0; p = 0.66), consistent with healthy ciliary beat frequencies in the literature 9 . Representative movies of the analysis are provided in the Supplementary Files (Supplementary Video 2).…”

Section: Resultssupporting

confidence: 82%

An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants

Martens

Amann

Schmidt

et al. 2019

Sci Rep

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Extremely premature infants are prone to severe respiratory infections, and the mechanisms underlying this exceptional susceptibility are largely unknown. Nasal epithelial cells (NEC) represent the first-line of defense and adult-derived ALI cell culture models show promising results in mimicking in vivo physiology. Therefore, the aim of this study was to develop a robust and reliable protocol for generating well-differentiated cell culture models from NECs of extremely premature infants. Nasal brushing was performed in 13 extremely premature infants at term corrected age and in 11 healthy adult controls to obtain NECs for differentiation at air-liquid interface (ALI). Differentiation was verified using imaging and functional analysis. Successful isolation and differentiation was achieved for 5 (38.5%) preterm and 5 (45.5%) adult samples. Preterm and adult ALI-cultures both showed well-differentiated morphology and ciliary function, however, preterm cultures required significantly longer cultivation times for acquiring full differentiation (44 ± 3.92 vs. 23 ± 1.83 days; p < 0.0001). Moreover, we observed that recent respiratory support may impair successful NECs isolation. Herewithin, we describe a safe, reliable and reproducible method to generate well-differentiated ALI-models from NECs of extremely premature infants. These models provide a valuable foundation for further studies regarding immunological and inflammatory responses and respiratory disorders in extremely premature infants.

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Section: Resultssupporting

confidence: 82%

An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants

Martens

Amann

Schmidt

et al. 2019

Sci Rep

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“…Future work will be focused on imaging of oviduct contraction with the oocytes or the cumulus-oocyte complexes presented to detail the oocyte transport process in 3D in vivo , as well as to determine the specific role of smooth muscle contraction in delivering oocytes to the fertilization site and transferring preimplantation embryos toward the uterus. There have been a number of recent studies investigating cilia dynamics with OCT [18, 36–39], with a major focus on the airway cilia. Some of these methods can be implemented using described approach toward detailed assessment of the cilia beat in the oviduct in correlation with the fluid flow and oocyte movements, which will provide direct in vivo analysis on the cilia function in mammalian reproduction.…”

Section: Resultsmentioning

confidence: 99%

Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window

Wang

Syed

Grishina

et al. 2018

Journal of Biophotonics

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The oviduct (or fallopian tube) serves as an environment for gamete transport, fertilization and preimplantation embryo development in mammals. Although there has been increasing evidence linking infertility with disrupted oviduct function, the specific roles that the oviduct plays in both normal and impaired reproductive processes remain unclear. The mouse is an important mammalian model to study human reproduction. However, most of the current analyses of the mouse oviduct rely on static histology or 2D visualization, and are unable to provide dynamic and volumetric characterization of this organ. The lack of imaging access prevents longitudinal live analysis of the oviduct and its associated reproductive events, limiting the understanding of mechanistic aspects of fertilization and preimplantation pregnancy. To address this limitation, we report a 3D imaging approach that enables prolonged functional assessment of the mouse oviduct in vivo. By combining optical coherence tomography with a dorsal imaging window, this method allows for extended volumetric visualization of the oviduct dynamics, which was previously not achievable. The approach is used for quantitative analysis of oviduct contraction, spatiotemporal characterization of cilia beat frequency and longitudinal imaging. This new approach is a useful in vivo imaging platform for a variety of live studies in mammalian reproduction.

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“…According to the previous publication, the phase resolved Doppler (PRD) method is able to visualize the ciliary motion by analyzing the interferometric data [27]. Specifically, the PRD algorithm defines a linear relationship between the ciliary displacement, ∆d, and the temporal phase change, ∆ϕ, of the interferogram.…”

Section: Data Processingmentioning

confidence: 99%

“…The depth resolved imaging capabilities of OCT allows for access to the inner cavity of the fallopian tube through the open abdominal cavity, and the CBF is measured by analyzing the intensity fluctuations at ciliated sites [22,23]. Although endoscopic OCT has the potential for in-vivo oviduct CBF imaging, it is still insufficient to visualize the complex patterns and synchronicity of ciliary behavior due to the limited resolution in resolving the ciliary strokes on the micron level [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, a PRD-enhanced technique will be a better candidate for ciliary motion detection. In particular, our group has recently developed a PRD-OCT system for the nanometer-scale visualization of the ciliary beating cycles and validated the feasibility of using the PRD method to investigate the spatial and temporal behaviors of the cilium [27]. In addition, since ciliary activity naturally occurs on the surface of the tissue, cross-sectional imaging techniques, such as OCT, may not provide the most suitable field of view (FOV).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of oviduct ciliary beat frequency using real time phase resolved Doppler spectrally encoded interferometric microscopy

He¹,

Qu²,

Jing³

et al. 2019

Biomed. Opt. Express

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Ciliary activity, characterized by the coordinated beating of ciliary cells, generates the primary driving force for oviduct tubal transport, which is an essential physiological process for successful pregnancies. Malfunction of the cilium in the fallopian tube, or oviduct, may increase the risk of infertility and tubal pregnancy that can result in maternal death. While many ex-vivo studies have been carried out using bright field microscopy, this technique is not feasible for the in-vivo investigation of oviduct ciliary beating frequency (CBF). Optical coherence tomography (OCT) has been able to provide in-vivo CBF imaging in a mouse model, but its resolution may be insufficient to resolve the spatial and temporal features of the cilium. Our group has recently developed the phase resolved Doppler (PRD) OCT method to visualize ciliary strokes at ultra-high displacement sensitivity. However, the cross-sectional field of view (FOV) may not be ideal for visualizing the surface dynamics of ciliated tissue. In this study, we report on the development of phase resolved Doppler spectrally encoded interferometric microscopy (PRD-SEIM) to visualize the oviduct ciliary activity within an en face FOV. This novel real time imaging system offers micrometer spatial resolution, sub-nanometer displacement sensitivity, and the potential for in-vivo endoscopic adaptation. The feasibility of the approach has been validated through ex-vivo experiments where the porcine oviduct CBF has been measured across different temperature conditions and the application of a drug. CBF ranging from 8 to 12 Hz have been observed at different temperatures, while administration of lidocaine decreased the CBF and deactivated the motile cilia. This study will serve as a stepping stone to in-vivo oviduct ciliary endoscopy and future clinical translations.

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Visualization and Detection of Ciliary Beating Pattern and Frequency in the Upper Airway using Phase Resolved Doppler Optical Coherence Tomography

Cited by 32 publications

References 49 publications

An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants

An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants

Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window

Characterization of oviduct ciliary beat frequency using real time phase resolved Doppler spectrally encoded interferometric microscopy

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