Visualization of cyclic nucleotide dynamics in neurons

Gorshkov, Kirill; Zhang, Jin

doi:10.3389/fncel.2014.00395

Cited by 22 publications

(16 citation statements)

References 86 publications

(93 reference statements)

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“…The recent establishment of genetically encoded cAMP fluorescent indicators has broadened the number of possible approaches (Gorshkov and Zhang, 2014;Calebiro and Maiellaro, 2014). The Förster resonance energy transfer (FRET)-based sensor Epac1-camps is a notable example (Nikolaev et al, 2004;Börner et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Odor induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Miazzi

Hansson

Wicher

2016

Journal of Experimental Biology

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Insect odorant receptors are seven transmembrane domain proteins that form cation channels, whose functional properties such as receptor sensitivity are subject to regulation by intracellular signaling cascades. Here, we used the cAMP fluorescent indicator Epac1-camps to investigate the occurrence of odor-induced cAMP production in olfactory sensory neurons (OSNs) of Drosophila melanogaster. We show that stimulation of the receptor complex with an odor mixture or with the synthetic agonist VUAA1 induces a cAMP response. Moreover, we show that while the intracellular Ca 2+ concentration influences cAMP production, the OSN-specific receptor OrX is necessary to elicit cAMP responses in Ca 2+ -free conditions. These results provide direct evidence of a relationship between odorant receptor stimulation and cAMP production in olfactory sensory neurons in the fruit fly antenna and show that this method can be used to further investigate the role that this second messenger plays in insect olfaction.

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Section: Introductionmentioning

confidence: 99%

Odor induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Miazzi

Hansson

Wicher

2016

Journal of Experimental Biology

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“…With the continuing development of fluorescent biosensors responsive to physiological levels of cGMP (Gorshkov & Zhang, ) it is becoming possible to visualize NO signalling through its receptors in real time and even in small synaptic dimensions (Bhargava et al . ).…”

Section: Introductionmentioning

confidence: 99%

From synaptically localized to volume transmission by nitric oxide

Garthwaite

2015

The Journal of Physiology

102

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Nitric oxide (NO) functions widely as a transmitter/diffusible second messenger in the central nervous system, exerting physiological effects in target cells by binding to specialized guanylyl cyclase-coupled receptors, resulting in cGMP generation. Despite having many context-dependent physiological roles and being implicated in numerous disease states, there has been a lack of clarity about the ways that NO operates at the cellular and subcellular levels. Recently, several approaches have been used to try to gain a more concrete, quantitative understanding of this unique signalling pathway. These approaches have included analysing the kinetics of NO receptor function, real-time imaging of cellular NO signal transduction in target cells, and the use of ultrasensitive detector cells to record NO as it is being generated from native sources in brain tissue. The current picture is that, when formed in a synapse, NO is likely to act only very locally, probably mostly within the confines of that synapse, and to exist only in picomolar concentrations. Nevertheless, closely neighbouring synapses may also be within reach, raising the possibility of synaptic crosstalk. By engaging its enzyme-coupled receptors, the low NO concentrations are able to stimulate physiological (submicromolar) increases in cGMP concentration in an activity-dependent manner. When many NO-emitting neurones or synapses are active simultaneously in a tissue region, NO can act more like a volume transmitter to influence, and perhaps coordinate, the behaviour of cells within that region, irrespective of their identity and anatomical connectivity.

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“…More interestingly, co-application of spectrum separable light-regulated cyclases and light-regulated PDEs would enable bi-directional control of the level of cNMP, either at close or discrete locations. In addition, numerous genetically encoded cAMP and cGMP sensors confer optical visualization of the distribution and dynamics of cAMP and cGMP [ 106 , 107 ]. The combination of spectrum compatible light-gated cyclases, PDEs with fluorescent biosensors [ 108 ] holds great promise to simultaneously manipulate and map the dynamics of cNMP signaling in live cells in a superior precise and quantitative manner.…”

Section: Applications Of Light-gated Pdesmentioning

confidence: 99%

Advances, Perspectives and Potential Engineering Strategies of Light-Gated Phosphodiesterases for Optogenetic Applications

Tian

Yang

Gao

2020

IJMS

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The second messengers, cyclic adenosine 3′-5′-monophosphate (cAMP) and cyclic guanosine 3′-5′-monophosphate (cGMP), play important roles in many animal cells by regulating intracellular signaling pathways and modulating cell physiology. Environmental cues like temperature, light, and chemical compounds can stimulate cell surface receptors and trigger the generation of second messengers and the following regulations. The spread of cAMP and cGMP is further shaped by cyclic nucleotide phosphodiesterases (PDEs) for orchestration of intracellular microdomain signaling. However, localized intracellular cAMP and cGMP signaling requires further investigation. Optogenetic manipulation of cAMP and cGMP offers new opportunities for spatio-temporally precise study of their signaling mechanism. Light-gated nucleotide cyclases are well developed and applied for cAMP/cGMP manipulation. Recently discovered rhodopsin phosphodiesterase genes from protists established a new and direct biological connection between light and PDEs. Light-regulated PDEs are under development, and of demand to complete the toolkit for cAMP/cGMP manipulation. In this review, we summarize the state of the art, pros and cons of artificial and natural light-regulated PDEs, and discuss potential new strategies of developing light-gated PDEs for optogenetic manipulation.

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Visualization of cyclic nucleotide dynamics in neurons

Cited by 22 publications

References 86 publications

Odor induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Odor induced cAMP production in Drosophila melanogaster olfactory sensory neurons

From synaptically localized to volume transmission by nitric oxide

Advances, Perspectives and Potential Engineering Strategies of Light-Gated Phosphodiesterases for Optogenetic Applications

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