Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis

Anker, Pálma; Fésűs, Luca; Kiss, Norbert; Noll, Judit; Becker, Krisztina; Kuroli, Enikő; Mayer, Balázs; Bozsányi, Szabolcs; Lőrincz, Kende; Lihacova, Ilze; Lihachev, Alexey; Lange, Marta; Wikonkál, Norbert; Medvecz, Márta

doi:10.3390/s21041105

Cited by 10 publications

(9 citation statements)

References 25 publications

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“…An LED-based MSI device was recently successfully introduced for the detection of recurring skin cancers [43,44] and proved to be applicable in the differentiation of different tumor types from benign lesions based on their mean intensity of autofluorescence (AF) [45]. In addition, MSI has been also utilized for the detection of rare skin disorders [46,47].…”

Section: Figurementioning

confidence: 99%

Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis

et al. 2021

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Melanoma is a melanocytic tumor that is responsible for the most skin cancer-related deaths. By contrast, seborrheic keratosis (SK) is a very common benign lesion with a clinical picture that may resemble melanoma. We used a multispectral imaging device to distinguish these two entities, with the use of autofluorescence imaging with 405 nm and diffuse reflectance imaging with 525 and 660 narrow-band LED illumination. We analyzed intensity descriptors of the acquired images. These included ratios of intensity values of different channels, standard deviation and minimum/maximum values of intensity of the lesions. The pattern of the lesions was also assessed with the use of particle analysis. We found significantly higher intensity values in SKs compared with melanoma, especially with the use of the autofluorescence channel. Moreover, we found a significantly higher number of particles with high fluorescence in SKs. We created a parameter, the SK index, using these values to differentiate melanoma from SK with a sensitivity of 91.9% and specificity of 57.0%. In conclusion, this imaging technique is potentially applicable to distinguish melanoma from SK based on the analysis of various quantitative parameters. For this application, multispectral imaging could be used as a screening tool by general physicians and non-experts in the everyday practice.

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Section: Figurementioning

confidence: 99%

Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis

et al. 2021

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“…Our research group has previously shown this technique to differentiate seborrheic keratosis from melanoma [ 33 ]. In addition, we introduced MSI for the diagnostics of rare skin disorders, including pseudoxanthoma elasticum [ 34 ] and keratinopathic ichthyosis [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Multispectral Imaging Algorithm Predicts Breslow Thickness of Melanoma

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Farkas

et al. 2021

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Breslow thickness is a major prognostic factor for melanoma. It is based on histopathological evaluation, and thus it is not available to aid clinical decision making at the time of the initial melanoma diagnosis. In this work, we assessed the efficacy of multispectral imaging (MSI) to predict Breslow thickness and developed a classification algorithm to determine optimal safety margins of the melanoma excision. First, we excluded nevi from the analysis with a novel quantitative parameter. Parameter s’ could differentiate nevi from melanomas with a sensitivity of 89.60% and specificity of 88.11%. Following this step, we have categorized melanomas into three different subgroups based on Breslow thickness (≤1 mm, 1–2 mm and >2 mm) with a sensitivity of 78.00% and specificity of 89.00% and a substantial agreement (κ = 0.67; 95% CI, 0.58–0.76). We compared our results to the performance of dermatologists and dermatology residents who assessed dermoscopic and clinical images of these melanomas, and reached a sensitivity of 60.38% and specificity of 80.86% with a moderate agreement (κ = 0.41; 95% CI, 0.39–0.43). Based on our findings, this novel method may help predict the appropriate safety margins for curative melanoma excision.

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“…Neben der Dermatoskopie wurden weitere neuartige bildgebende Verfahren wie die nichtlineare optische Mikroskopie und die multispektrale Bildgebung als neuartige quantitative Instrumente zur Bewertung von PXE-befallener Haut eingesetzt [ 10 , 15 , 21 ]. Diese Techniken können auch für die Beurteilung einer breiteren Palette von Erbkrankheiten eingesetzt werden, darunter das Ehlers-Danlos-Syndrom, keratinopathische Ichthyose und das Marfan-Syndrom [ 2 , 6 , 16 , 20 ]. Die multimodale Bildgebung mit der Kombination von Dermatoskopie und verschiedenen bildgebenden Verfahren könnte ein weiterer Ansatz für die gründliche Untersuchung der Haut von PatientInnen mit Genodermatosen sein, der in der Diagnostik und Therapieüberwachung dieser Erkrankungen eingesetzt werden kann.…”

Section: Diskussionunclassified

Dermoscopy of genodermatoses

et al. 2023

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ZusammenfassungGenodermatosen sind eine Gruppe erblicher Hautkrankheiten, deren Diagnose aufgrund ihrer Seltenheit sowie klinischen und genetischen Vielfalt eine Herausforderung darstellt. Die meisten Genodermatosen werden autosomal oder X‑chromosomal vererbt, auch Mosaikformen werden beobachtet. Genodermatosen umfassen verschiedene Phänotypen, die von einer begrenzten kutanen Erkrankung bis hin zu einer schweren kutanen und extrakutanen Beteiligung reichen. Sie können auch Zeichen multisystemischer Störungen sein. Trotz der jüngsten Fortschritte in der Gentechnologie und den bildgebenden Verfahren der Haut stellt die Dermatoskopie für das Screening, die Diagnostik und die therapeutische Nachsorge eine nützliche Untersuchungsmethode dar. Bei der ektopischen Mineralisierungsstörung Pseudoxanthoma elasticum und der lysosomalen Speicherkrankheit Morbus Fabry können die Symptome auf der Haut auf Beteiligungen innerer Organe hinweisen. Bei Keratinisierungskrankheiten wie Ichthyosen und akantholytischen Dyskeratosen wie Morbus Darier und Morbus Hailey-Hailey kann die Dermatoskopie helfen, die Wirksamkeit der Therapie zu zeigen, indem sie Hintergrunderythem, Hyperkeratose und den interkeratinozytären Raum sichtbar macht. Die Dermatoskopie ist eine nichtinvasive In-vivo-Untersuchung, die in der allgemeinen Dermatologie zunehmend an Bedeutung gewinnt und ein nützliches, leicht zugängliches Instrument zur Erkennung charakteristischer Merkmale von Genodermatosen sein kann.

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Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis

Cited by 10 publications

References 25 publications

Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis

Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis

Multispectral Imaging Algorithm Predicts Breslow Thickness of Melanoma

Dermoscopy of genodermatoses

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