Visualization of micro-scale inhomogeneities in acrylic thickener solutions: A multiple particle tracking study

Kowalczyk, Anne; Oelschlaeger, Claude; Willenbacher, Norbert

doi:10.1016/j.polymer.2014.12.041

Cited by 35 publications

(35 citation statements)

References 46 publications

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“…The apparent viscosity h app determined from the average MSD trace was 1.65 AE 0.65 mPa s and this value is in good agreement with the viscosity of pure water. 22 In the hydrogels GDH-SC/Gre2p-ST 2 (Fig. 3b) and GDH-SC/ Gre2p-ST 3 (Fig.…”

Section: Construction and Characterization Of The Enzyme Hydrogelsmentioning

confidence: 96%

Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels

et al. 2019

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Section: Construction and Characterization Of The Enzyme Hydrogelsmentioning

confidence: 96%

Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels

et al. 2019

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“…As compared to chemically crosslinked polymers, the gelation of the enzyme hydrogels occurs rather slowly, similar as observed for synthetic elastin hydrogels, where the polymerising proteins first form discrete particles that merge to form larger spheres and further coalesce into open linked networks of a porous hydrogel network 31 . To further elucidate their material properties, the enzyme hydrogels were analysed by optical microrheology based on multiple particle tracking (MPT) analysis 32 (Supplementary Figure 4). The method revealed that the hydrogel has a homogeneous structure on the micrometer length scale with a G0= 20±7 Pa, an average mesh size = 60±7 nm and a pore size <200 nm that is in the range of typical microfiltration membranes 33,34 .…”

Section: Resultsmentioning

confidence: 99%

Self-assembling all-enzyme hydrogels for biocatalytic flow processes

Peschke

Gallus

Bitterwolf

et al. 2017

Preprint

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______________________________________________________________We describe the construction of binary self-assembling all-enzyme hydrogels that are comprised entirely of two tetrameric globular enzymes, the stereoselective dehydrogenase LbADH and the cofactor-regenerating glucose 1-dehydrogenase GDH. The enzymes were genetically fused with a SpyTag or SpyCatcher domain, respectively, to generate two complementary homo-tetrameric building blocks that polymerise under physiological conditions into porous hydrogels. The biocatalytic gels were used for the highly stereoselective reduction of a prochiral diketone substrate where they showed the typical behaviour of the coupled kinetics of coenzyme regenerating reactions in the substrate channelling regime. They effectively sequestrate the NADPH cofactor even under continuous flow conditions. Owing to their sticky nature, the gels can be readily mounted in simple microfluidic reactors without the need for supportive membranes. The reactors revealed extraordinary high space-time yields with nearly quantitative conversion (>95%), excellent stereoselectivity (d.r. > 99:1), and total turnover numbers of the expensive cofactor NADP(H) that are amongst the highest values ever reported. ______________________________________________________________

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“…We used multi particle tracking (MPT) microrheology, i.e., we simultaneously tracked the Brownian motion of several hundred fluorescent tracer particles added to a colloidal suspension. This technique was originally developed to study cell biology systems 18 19 , but was later applied to study heterogeneity, e.g., in clay suspensions 20 21 , polymeric thickener solutions 22 23 , and agarose 24 and food gels 25 . Here, we applied it to highly turbid colloidal suspensions for the first time.…”

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confidence: 99%

Microstructure, local dynamics, and flow behavior of colloidal suspensions with weak attractive interactions

Weis

Oelschlaeger

Dijkstra

et al. 2016

Sci Rep

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We present a comprehensive micro- and macrorheological study of the effect of weak depletion attraction (Ψdep ≈ 1–10 kBT) on dense colloidal suspensions stabilized by short-range repulsive interactions. We used aqueous polymer dispersions as model system and demonstrated the unique capabilities of multiple particle tracking (MPT) to disclose structural changes in such technically important systems exhibiting many characteristic features of hard sphere systems. Below the hard sphere freezing point ϕc, viscosity increases monotonically with increasing Ψdep due to the transition from a fluid to a fluid/crystalline and finally to a gel state. Above ϕc, increasing attraction strength first results in a viscosity reduction corresponding to the formation of large, permeable crystals and then in a viscosity increase when a network of dense, small crystals forms. The fraction of the fluid and crystal phase, particle concentration in each phase as well as the modulus of the micro-crystals are obtained, the latter decreases with Ψdep. Above the colloidal glass transition strong heterogeneities and different local particle mobility in the repulsive and attractive arrested states are found. Particles are trapped in the cage of neighboring particles rather than in an attractive potential well. The intermediate ergodic state exhibits uniform tracer diffusivity.

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Visualization of micro-scale inhomogeneities in acrylic thickener solutions: A multiple particle tracking study

Cited by 35 publications

References 46 publications

Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels

Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels

Self-assembling all-enzyme hydrogels for biocatalytic flow processes

Microstructure, local dynamics, and flow behavior of colloidal suspensions with weak attractive interactions

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