Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors

Sanfeliu-Cerdán, Neus; Lin, Li-Chun; Dunn, Alexander R.; Goodman, Miriam B.; Krieg, Michael

doi:10.1007/978-1-0716-2851-5_16

Cited by 8 publications

(15 citation statements)

References 66 publications

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“…These experiments are commonly performed in a dual optical trap, dumbbell configuration, in which a droplet is sandwiched between two trapped, active and passive microspheres (Fig. 3, [14, 40]). The complex shear modulus and surface tension of the material is then extracted from the active force measurement and passive bead displacement, with the assumption that the microsphere radius is considerably smaller than the protein droplet and that the viscosity of the dilute phase is known [41].…”

Section: Resultsmentioning

confidence: 99%

Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology

Català-Castro,

Ortiz-Vásquez,

Martínez-Fernández

et al. 2023

Preprint

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Recording the mechanical response of biological samples, the cell's interior and complex fluids in general, would enable deeper understanding of cellular differentiation, ageing and drug discovery. Here, we present a time-shared optical tweezer microrheology (TimSOM) pipeline to determine the frequency- and age-dependent viscoelastic properties of biological materials. Our approach consists in splitting a single laser beam into two near-instantaneous time-shared optical traps to carry out simultaneous force and displacement measurements with sub-nanometer and sub-picoNewton accuracy during sinusoidal perturbations. Leveraging numerical and analytical models, we find solutions to commonly encountered deviations, to build an artefact-free nanorheometer. We demonstrate the versatility of the technique by 1) measuring the phase transitions of an ageing biomolecular condensate, 2) quantifying the complex viscoelastic properties of three intracellular compartments of zebrafish progenitor cells, and, using Caenorhabditis elegans, we uncover how mutations causing nuclear envelopathies soften the cytosol of intestinal cells during organismal age. Together, our advances afford rapid phenotyping of material properties inside cells and proteins blends, opening avenues for biomedical and drug screening applications.

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Section: Resultsmentioning

confidence: 99%

Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology

Català-Castro,

Ortiz-Vásquez,

Martínez-Fernández

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Optogenetics techniques can help to decipher the sensing of mechanical forces acting on cells and neurons. These mechanical stresses can be sensed by specialized force sensors located in the cell membrane or the intracellular compartments …”

Section: Light-based Neurosciencementioning

confidence: 99%

“…These mechanical stresses can be sensed by specialized force sensors located in the cell membrane or the intracellular compartments. 13 Foundation of Optogenetics in Mechanobiology. A wide range of new optical technologies, exploiting the very nature of light, have been integrated to conventional microscopes reaching unprecedented flexibility, precision, and depth for the visualization of neuronal processes and networks as described in a previous section.…”

Section: ■ Structured Light In Biosystemsmentioning

confidence: 99%

“…Optogenetics in combination with Fluorescence Resonance Energy Transfer (FRET) helps to decipher the sensing of mechanical forces acting on cells and neurons. These mechanical stresses can be sensed by specialized force sensors located in the cell membrane or the intracellular compartments . We show how structured light can be utilized in neurosciences, in particular in optogenetics for studies of neuronal networks.…”

Section: Introduction To Optical Mechanobiologymentioning

confidence: 99%

“…These mechanical stresses can be sensed by specialized force sensors located in the cell membrane or the intracellular compartments. 13 We show how structured light can be utilized in neurosciences, in particular in optogenetics for studies of neuronal networks.…”

Section: ■ Introduction To Optical Mechanobiologymentioning

confidence: 99%

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Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond

Stilgoe,

Favre-Bulle,

Watson

et al. 2024

ACS Photonics

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Mechanobiology helps us to decipher cell and tissue functions by looking at changes in their mechanical properties that contribute to development, cell differentiation, physiology, and disease. Mechanobiology sits at the interface of biology, physics and engineering. One of the key technologies that enables characterization of properties of cells and tissue is microscopy. Combining microscopy with other quantitative measurement techniques such as optical tweezers and scissors, gives a very powerful tool for unraveling the intricacies of mechanobiology enabling measurement of forces, torques and displacements at play. We review the field of some light based studies of mechanobiology and optical detection of signal transduction ranging from optical micromanipulation�optical tweezers and scissors, advanced fluorescence techniques and optogenentics. In the current perspective paper, we concentrate our efforts on elucidating interesting measurements of forces, torques, positions, viscoelastic properties, and optogenetics inside and outside a cell attained when using structured light in combination with optical tweezers and scissors. We give perspective on the field concentrating on the use of structured light in imaging in combination with tweezers and scissors pointing out how novel developments in quantum imaging in combination with tweezers and scissors can bring to this fast growing field.

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Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans

Martin,

Bent,

Hill

et al. 2024

Developmental Cell

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Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors

Cited by 8 publications

References 66 publications

Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology

Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology

Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond

Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans

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