Visualizing the distribution of curcumin in the root of <i>Curcuma longa via</i> VUV-postionization mass spectrometric imaging

Nie, Wuyi; Lu, Qiao; Hu, Tao; Xie, Min; Hu, Yongjun

doi:10.1039/d2an01516a

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…A home-made TOF-MS system was used in this study. The details of the apparatus have been described previously, and only a brief introduction is given here [39]. In this experiment, the laser light sources used included two nanosecond-scale solidstate lasers.…”

Section: Ldpi-tof-msi Apparatusmentioning

confidence: 99%

Visual Analysis of Carbendazim Residues in Carrot Tubers via Postionization Mass Spectrometry Imaging

Wang,

Zhang,

2024

Applied Sciences

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Carbendazim (CBZ) residues in food are a severe threat to food safety, and their detection is a challenging problem in food science. We introduce here a new method based on laser desorption postionization mass spectrometry imaging (LDPI-MSI) for detecting CBZ residues in carrots. In the novel LDPI-MSI method, two distinct laser beams simultaneously exert dissociation and ionization, which offers several advantages over traditional techniques based on single-photon matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), including simplified sample preparation, streamlined operation workflow, and a lower limit of detection (LOD). The LOD, in the proposed method, has been lowered to 0.019 ppm. Coupled with mass spectrometry imaging (MSI), the LDPI-MS method enabled in situ detection of small molecular compounds, such as chemical pesticides, and provided comprehensive and accurate results. The image obtained from the characteristic mass spectrometric signature of CBZ at m/z 191 illustrated that most of the CBZ could not enter the carrot tubers directly, but a small amount of CBZ entered the carrot root and was mainly concentrated in the central xylem. The results suggest that the proposed method could potentially be used in pesticide analysis.

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Section: Ldpi-tof-msi Apparatusmentioning

confidence: 99%

Visual Analysis of Carbendazim Residues in Carrot Tubers via Postionization Mass Spectrometry Imaging

Wang,

Zhang,

2024

Applied Sciences

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“…Nie et al introduced a vacuum-ultraviolet laser to post-ionize the laser-desorbed materials at the base of MALDI. They visualized the distribution of curcumin in the turmeric root at different maturity periods, but the sample must be put in the high vacuum chamber [ 18 ]. Meng et al developed a 5 μm-resolution laser ablation electrospray ionization-MSI by using a microlensed fiber and visualized metabolites in a parsnip root section [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging

Xiao,

Guan,

et al. 2024

Metabolites

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With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption–dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an atmospheric environment and requires minimal sample preparation. This study presents images of in-situ metabolic profiling of two kinds of plants from different origins, a wild and a farmed Rheum palmatum L. From the screen of these two root sections, the wild one presented five more endogenous molecules than the farmed one, which provides information about the differences in metabolomics.

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“…(2) Spotting a standard of known concentration on a control tissue section. [2][3][4][5][6][7] (3) A method of preparing two serial sections of a sample, one measured by MSI and the other with the extracted liquid by liquid chromatography -mass spectrometry (LC-MS/MS) [8][9][10][11][12][13][14][15][16] or gas chromatography-coupled mass spectrometry (GC-MS). 17) (4) A method in which standards are added to tissue homogenates and frozen blocks are prepared, followed by the same pretreatment as for real samples.…”

Section: Introductionmentioning

confidence: 99%

An Alternative Method for Quantitative Mass Spectrometry Imaging (<i>q</i>-MSI) of Dopamine Utilizing Fragments of Animal Tissue

Nagano,

Odake,

Shimma

2023

Mass Spectrometry

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Mass spectrometry imaging (MSI) is a well-known method for the ionization of molecules on tissue sections and the visualization of their localization. Recently, different sample preparation methods and new instruments have been used for MSI, and different molecules are becoming visible. On the other hand, although several quantification methods ( q -MSI) have been proposed, there is still room for the development of a simplified procedure. Here, we have attempted to develop a reproducible and reliable quantification method using a calibration curve prepared from tissue debris of a frozen section of a sample when we trim the frozen blocks. We discuss the reproducibility of this method across different sample lots and the effect of the biological matrix (ion suppression) on our results. The quantitative performance was evaluated in terms of accuracy and relative standard deviation, and the reliability of the quantitative values obtained by matrix-assisted laser desorption/ionization-MSI was further evaluated by enzyme-linked immunosorbent assay (ELISA). Our q -MSI method for the quantification of dopamine in mouse brain tissue was found to be highly linear, accurate, and precise. The quantitative values obtained by MSI were found to be highly comparable (>85% similarity) to the results obtained by ELISA from the same tissue extracts.

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Visualizing the distribution of curcumin in the root of Curcuma longa via VUV-postionization mass spectrometric imaging

Abstract: Curcumin is a dietary spice and coloring agent widely used in food and herbal medicine. Herein, we visualized the distribution of curcumin in fresh Curcuma longa (turmeric) root sections by...

Cited by 5 publications

References 41 publications

Visual Analysis of Carbendazim Residues in Carrot Tubers via Postionization Mass Spectrometry Imaging

Visual Analysis of Carbendazim Residues in Carrot Tubers via Postionization Mass Spectrometry Imaging

In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging

An Alternative Method for Quantitative Mass Spectrometry Imaging (<i>q</i>-MSI) of Dopamine Utilizing Fragments of Animal Tissue

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