Vital pulpectomy and root filling with N2 or Endomethasone

Abstract

Summary. Using an aseptic and standardized endodontic technique, vital pulpectomy was performed on 16 pairs of human permanent maxillary front teeth scheduled for extraction in connection with prosthetic treatment. All the teeth had clinically sound pulps before treatment and 16 teeth were root filled with N2 and the other 16 with Endomethasone. In all cases except two, contralateral teeth were used. After 4–8 months the teeth were extracted and processed for histological examination using light microscopy. Histological examination of the residual pulp demonstrated no statistically significant differences between the two root‐filling materials, concerning inflammation and the frequency of necrosis. The healing reactions, occurrence of fibrous tissue in the residual pulp and hard tissue apposition on the root canal wall, were more pronounced after root filling with N2, than after Endomethasone, but the differences were not statistically significant. Comparing the results with those after vital pulpectomy and root filling with zinc oxide‐eugenol cement, total necrosis of the residual pulp was most frequent after root filling with Endomethasone, less so after zinc oxide‐ eugenol cement and least after root filling with N2, but the differences were not statistically significant. When estimated from the local findings, that is the histological reactions in the residual pulp, no advantages could be observed by using N2 or Endomethasone, and owing to the risk of general side effects, because of the formaldehyde content and the complex composition of the two materials routine use of N2 or Endomethasone cannot be recommended in pulpectomy.

Up-regulation of Receptor Activator Nuclear Factor–Kappa B Ligand Expression by Root Canal Sealers in Human Osteoblastic Cells

“…Various histologic investigations indicate that components leaching from root canal sealer might induce mild to severe apical lesion (1)(2)(3)(4). This process involves a local inflammatory reaction.…”

“…The irritative effects of root canal sealers have been evaluated by histopathologic examinations of the tissue response. Unfortunately, several studies have shown that most commercially available root canal sealers can induce mild to severe inflammatory alteration within apical tissues (1)(2)(3)(4). Recently, our studies have shown the activation of cyclooxygenase (COX)-2 (5-7), interleukin (IL)-6 (8), , heme oxygenase-1 (9), gelatinases (10), and tissue type plasminogen activators (10) might play an important role in induction of periapical destruction by root canal sealers.…”

Effects of Root Canal Sealers on Alkaline Phosphatase in Human Osteoblastic Cells

“…Various histologic investigations indicate that commercially available root canal sealers tested in this study can induce apical bone destruction (1)(2)(3)(4). In this study, we first found that root canal sealers can suppress ALP mRNA gene expression and ALP activity in U2OS cells.…”

“…The irritative effects of root canal sealers have been evaluated by histopathologic examinations of the tissue response. Unfortunately, several studies have shown that most commercially available root canal sealers can induce mild to severe inflammatory alteration within apical tissues, leading to bone destruction (1)(2)(3)(4). Recently, our studies have shown the activation of inflammatory mediator cyclooxygenase-2 (5-7), inflammatory cytokines interleukin-6 and interleukin-8 (8), antioxidant enzyme heme oxygenase-1 (9), proteolytic enzyme gelatinases and tissue-type plasminogen activators (10), and osteolytic marker receptor activator of nuclear factor kappa B ligand (11) might play an important role in root canal sealer-induced periapical inflammation.…”

Testing endodontic sealers on human umbilical vein endothelial cells

“…With advances in etch-&-rinse and self-etching adhesive technologies [7,16], there is increasing interest in the use of low viscosity, methacrylate resin-based composites as sealers for root filling materials in endodontics [3,4,17]. The release of unreacted monomers from resin composites may stimulate the growth of bacteria [18] and promote allergic or cytotoxic reactions to intraoral tissues [18].…”