Vitamin A prevents round spermatid nuclear damage and promotes the production of motile sperm during<i>in vitro</i>maturation of vitrified pre-pubertal mouse testicular tissue

Dumont, Ludovic; Oblette, Antoine; Rondanino, Christine; Jumeau, Fanny; Bironneau, A.; Liot, D.; Duchesne, Véronique; Wils, Julien; Rives, Nathalie

doi:10.1093/molehr/gaw063

Cited by 33 publications

(38 citation statements)

References 58 publications

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“…The method is quite simple in that immature testis tissue pieces were placed on the agarose gel block which was half‐soaked in the culture media. This agarose gel culture method (AG) was repeated by several researchers and became a reliable culture method for in vitro spermatogenesis . However, the efficiency and duration of in vitro spermatogenesis were not comparable to those in vivo.…”

Section: Introductionmentioning

confidence: 99%

In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues

Komeya

Yamanaka

Sanjo

et al. 2019

Reprod Medicine & Biology

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Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air. In this condition, however, a tissue piece tends to round up to be compact, whose central region suffers from shortage of nutrients and oxygen. In this study, the authors improved the culture condition by spreading each tissue thin and flat, by which they were able to get better access to the oxygen and nutrients. Methods Immature mouse testis tissues placed on agarose gel block were forced to spread flat by covering with a polydimethylsiloxane (PDMS) ceiling chip (PC chip). They were then cultured for weeks and evaluated by the transgene expression of Acr‐Gfp, which reflects the progression of spermatogenesis. Results Testis tissues covered with PC chip initiated and maintained spermatogenesis in its wider region than those without PC chip covering. Flow cytometric analysis demonstrated that the PC method yielded more numerous meiotic germ cells than those without PC. Immunohistochemical examination confirmed the authentic histological figure of spermatogenesis from spermatogonia up to round or elongating spermatids. Conclusions The PC chip method is simple and effective to improve the efficiency of in vitro spermatogenesis in the organ culture system.

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Section: Introductionmentioning

confidence: 99%

In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues

Komeya

Yamanaka

Sanjo

et al. 2019

Reprod Medicine & Biology

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“…The agarose gel organ culture method became a reliable standard method, repeated by many independent research groups, to induce mouse spermatogenesis. [42][43][44][45] However, the efficiency and duration of the spermatogenesis were far from comparable to those observed in vivo. 46 To improve the culture condition, replicating the microcirculatory system of the body in the organ culture system could be effective.…”

Section: Microfluidic Systemmentioning

confidence: 90%

“…The agarose gel organ culture method became a reliable standard method, repeated by many independent research groups, to induce mouse spermatogenesis . However, the efficiency and duration of the spermatogenesis were far from comparable to those observed in vivo .…”

Section: Organ Culture Method Modern Developmentmentioning

confidence: 99%

In vitro spermatogenesis: A century‐long research journey, still half way around

Komeya

Sato

Ogawa

2018

Reprod Medicine & Biology

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BackgroundSpermatogenesis is one of the most complicated cellular differentiation processes in a body. Researchers struggled to find and develop a micro‐environmental condition that can support the process in vitro. Such endeavors can be traced back to a century ago and are yet continuing.MethodsReports on in vitro spermatogenesis and related works were selected and classified into four categories based on the method used; organ culture, tubule culture, cell culture, and 3‐dimensional cell culture methods. Each report was critically reviewed from the present point of view by authors who have been working on in vitro spermatogenesis with organ culture method over a decade.ResultsThe organ culture method has the longest history and is the most successful method, which produced fertile mouse sperm from spermatogonial stem cells. Formulation of the medium was a key factor, most importantly serum‐derived substances. However, factors in the serum that induce and support spermatogenesis in the cultured tissue remain to be identified. In addition, the success of mouse spermatogenesis is yet to be applied to other animals. On looking into the history of cell culture method, it became clear that Sertoli cells as feeder cells play an important role. Even with Sertoli cells, however, spermatogenic development has been limited to small parts of spermatogenesis, a segmented period of meiotic prophase for instance. Recent developments of organoid or 3‐dimensional culture techniques are promising but they still need further refinements.ConclusionThe study of in vitro spermatogenesis progressed significantly over the last century. We need more work, however, to establish a culture system that can induce and maintain complete spermatogenesis of many if not all mammalian species.

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“…; Dumont et al . ). On the other hand, the addition of carotenoids (lycopene, canthaxanthin, lutein) to freezing extenders exhibited significant ROS‐trapping and antioxidant properties preventing oxidative damage to frozen–thawed bovine (Tuncer et al .…”

Section: Introductionmentioning

confidence: 97%

“…Semen quality was improved by lycopene in healthy men (Zareba et al 2013) and by astaxanthin and bcar in goldfish (Carassius auratus; Tizkar et al 2015) when added to the diet. Vitamin A and retinol promoted the production of motile sperm during in vitro maturation (Arkoun et al 2015;Dumont et al 2016). On the other hand, the addition of carotenoids (lycopene, canthaxanthin, lutein) to freezing extenders exhibited significant ROS-trapping and antioxidant properties preventing oxidative damage to frozen-thawed bovine (Tuncer et al 2014;Tvrda et al 2017), ram (Souza et al 2017), boar (Varo-Ghiuru et al 2015 and fish sperm (Liu et al 2015) among others.…”

Section: Introductionmentioning

confidence: 99%

Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: antioxidant activity and bioactive properties on sperm cells

et al. 2019

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Aims To examine the antioxidant activity of Bacterioruberin (Bctr)‐rich extracts isolated from a hyperpigmented, genetically modified Haloferax volcanii strain (HVLON3) and to investigate the effect on cold‐sensitive ram sperm cells. Methods and Results The strain HVLON3 produces higher Bctr amounts than most haloarchaea (220 ± 13 mg g−1 DW). HVLON3‐Bctr extract has higher antioxidant activity than β‐carotene (threefold) as evaluated using 2,2 diphenyl‐1‐picrylhydrazyl combined with Electron Paramagnetic Resonance analysis (EC50 4·5 × 10−5 mol l−1 vs 13·9 × 10−5 mol l−1 respectively). Different concentrations of HVLON3‐Bctr extracts were assayed on ram sperm after freezing/thawing and physiologically relevant parameters were examined. Extracts containing 7 and 20 μmol l−1 Bctr significantly improved cell viability (P < 0·0001), total and progressive motility (P < 0·0001) and sperm velocities (P = 0·0172 for curvilinear velocity VCL, P = 0·0268 for average path velocity VAP and P = 0·0181 for straight line velocity VSL) and did not affect other parameters evaluated. Conclusions HVLON3 is an excellent source of natural microbial C50 carotenoids with applicability in Biotechnology, Biomedical and Veterinary fields. HVLON3 Bctr extract improves the quality of cryopreserved ram sperm cells and could be applied to increase insemination yields. Significance and Impact of the Study This study provides an insight on the bioactive properties of a bioproduct derived from haloarchaea (carotenoids) which are so far underexploited.

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Vitamin A prevents round spermatid nuclear damage and promotes the production of motile sperm duringin vitromaturation of vitrified pre-pubertal mouse testicular tissue

Cited by 33 publications

References 58 publications

In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues

In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues

In vitro spermatogenesis: A century‐long research journey, still half way around

Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: antioxidant activity and bioactive properties on sperm cells

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