Vitamin A Supplementation Transiently Increases Retinol Concentrations in Extrahepatic Organs of Neonatal Rats Raised under Vitamin A–Marginal Conditions

Hodges, Joanna; Tan, Libo; Green, Michael H.; Ross, A. Catharine

doi:10.3945/jn.116.234310

Cited by 10 publications

(30 citation statements)

References 30 publications

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“…It is also consistent with the relatively low retinol concentration in the skin, adipose tissue, and carcass (12,(24)(25)(26) and the low expression of lecithin-retinol acyltransferase (LRAT), an enzyme responsible for retinol storage, in the lungs during the neonatal period (27). Both the activity of LRAT and the uptake of CM-REs have been shown to be upregulated by retinoic acid (23,28).…”

Section: Tablesupporting

confidence: 63%

“…The preliminary findings of these studies indicate that, compared with adult rats in a similar VA status, the extrahepatic tissues in neonates store relatively little VA (13% compared with 44% in adult rats) (12), despite a higher proportion of retinyl esters (REs) being acquired by these tissues (40% compared with 25%) (13). VA supplementation increased the concentration of retinol in most extrahepatic organs, but only transiently (for w1 d) (12), suggesting that the extrahepatic tissues in neonates may have a limited retinol storage capacity.…”

Section: Introductionmentioning

confidence: 84%

“…Rats were housed and fed according to a protocol reported previously (12,16). Briefly, eleven pregnant Sprague-Dawley (Charles River Laboratories) rats were fed a VA-marginal (0.35 mg retinol equivalents/kg) AIN-93G purified diet (Research Diets) (17) to render their pups into a state similar to that of low-birth-weight infants in regions with a high prevalence of VA deficiency.…”

Section: Rats and Dietmentioning

confidence: 99%

“…The VA supplement consisted of all-trans retinyl palmitate (Sigma-Aldrich) in the amount of 50,000 IU/2.5 kg of body weight, as given to human infants (19), or 200 IU for a neonatal rat weighing 10 g. The proportion of labeled-to-unlabeled retinol in the supplemented dose was w0.05%. Doses were prepared and administered by using a method described in prior reports (12,16).…”

Section: Dose Administrationmentioning

confidence: 99%

“…Before calculating the retinol turnover rate [R(0,J) = L(0,J) 3 M(J)], organ models were simplified to merge the incoming chylomicron-and retinol-binding protein (RBP)-derived retinol into a common pool of total retinol ( Figure 2C). The mass of this pool [M(J)] was previously established by UPLC analysis (12).…”

Section: Model Structures and Kinetic Parametersmentioning

confidence: 99%

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Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Hodges

Tan

Green

et al. 2017

The American Journal of Clinical Nutrition

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Background: Vitamin A (VA; retinol) supplementation is used to reduce child mortality in countries with high rates of malnutrition. Existing research suggests that neonates (,1 mo old) may have a limited capacity to store VA in organs other than the liver; however, knowledge about VA distribution and kinetics in individual, nonhepatic organs is limited. Objective: We examined retinol uptake and turnover in nonhepatic organs, including skin, brain, and adipose tissue, in neonatal rats without and after VA supplementation. Design: Sprague-Dawley neonatal rats (n = 104) were nursed by mothers fed a VA-marginal diet (0.35 mg retinol/kg diet) and treated on postnatal day 4 with an oral dose of either VA (6 mg retinyl palmitate/g body weight) or canola oil (control), both containing 1.8 mCi of [ The RBP-ROH released from the liver was acquired mainly by the peripheral tissues but not retained efficiently, causing repeated recycling of retinol between plasma and tissues (541 compared with 5 times in the supplemented group and control group, respectively) and its rapid turnover in all organs, except the brain and white adipose tissue. Retinol stores in the liver lasted for w2 wk before being gradually transferred to other organs. Conclusions: VA supplementation administered in a single high dose during the first month after birth is readily acquired but not retained efficiently in peripheral tissues of neonatal rats, suggesting that a more frequent, lower-dose supplementation may be necessary to maintain steady VA concentrations in rapidly developing neonatal tissues.

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Section: Tablesupporting

confidence: 63%

Section: Introductionmentioning

confidence: 84%

Section: Rats and Dietmentioning

confidence: 99%

Section: Dose Administrationmentioning

confidence: 99%

Section: Model Structures and Kinetic Parametersmentioning

confidence: 99%

See 3 more Smart Citations

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Hodges

Tan

Green

et al. 2017

The American Journal of Clinical Nutrition

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Retinoic acid signalling adjusts tight junction permeability in response to air-liquid interface conditions

Lochbaum

Schilpp

Nonnenmacher

et al. 2020

Cellular Signalling

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Migratory capacity and function of dendritic cells from mesenteric afferent lymph nodes after feeding a single dose of vitamin A

Núñez

Barrios

Maccio-Maretto

et al. 2017

The Journal of Nutritional Biochemistry

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Lamina propria dendritic cells (DCs) have a permanent turnover with constitutive migration to mesenteric lymph nodes and replenishment by progenitors. Luminal bacteria and dietary constituents provide key signals that endow DCs their unique properties in vivo. Taking into account that the intestinal immune system is greatly influenced by retinoids, we evaluated in B6 mice 3, 8, 16 and 24 h after feeding a single dose of vitamin A phenotype and function of cells present in mesenteric afferent lymph nodes as well as signals involved in migration. We studied the frequency of CD11c+MHC-II+CD103+CD86+ and RALDH+ DCs by flow cytometry, we determined CCL-21 and D6 levels in tissue homogenates by Western blot, and we co-cultured cells isolated from afferent lymphatics with sorted CD4+ lymphocytes to assess Foxp-3 induction and homing receptor expression. Sixteen hours after vitamin A administration, DCs isolated from afferent lymphatics were able to induce homing receptors and Foxp3 expression in CD4+ lymphocytes. Our results show that a single dose of vitamin A generated a stream of signals and amplified the tolerogenic activity of DCs migrating to lymphoid tissue.

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Vitamin A Supplementation Transiently Increases Retinol Concentrations in Extrahepatic Organs of Neonatal Rats Raised under Vitamin A–Marginal Conditions

Abstract: Background: Vitamin A (VA; retinol) supplementation is recommended for children aged >6 mo in countries with high

Cited by 10 publications

References 30 publications

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Retinoic acid signalling adjusts tight junction permeability in response to air-liquid interface conditions

Migratory capacity and function of dendritic cells from mesenteric afferent lymph nodes after feeding a single dose of vitamin A

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