Vitamin C Deficiency Activates the Purine Nucleotide Cycle in Zebrafish

Kirkwood, Jay S.; Lebold, Katie M.; Miranda, Cristobal L.; Wright, Charlotte L.; Miller, Galen W.; Tanguay, Robert L.; Barton, Carrie L.; Traber, Maret G.; Stevens, Jan F.

doi:10.1074/jbc.m111.316018

Cited by 60 publications

(74 citation statements)

References 41 publications

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“…Briefly, juvenile zebrafish (~1 month of age)were divided into 3 groups and exclusively fed one of three designated diets: E−, E+ or Lab. Defined diets have been previously described (Lebold et al, 2011; Kirkwood et al, 2012; Miller et al, 2012), and similar diets were used (Table 1) with slightly lower ascorbic acid concentrations (350 mg/kg diet, added as Stay-C, Vitamin C-3, Argent Chemical Laboratories Inc., Redmond, WA, USA). The E+ diet contained 500 mg RRR-α-tocopherol/kg diet; the E− was made without added tocopherol.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously reported outcomes fromz ebrafish fed a diet, which has fully defined components sufficient for zebrafish health (Lebold et al, 2011; Kirkwood et al, 2012; Miller et al, 2012). This diet has permitted the controlled removal of key nutrients from the parental diet, allowing the use of zebrafish to query nutrition-related questions.…”

Section: Introductionmentioning

confidence: 99%

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The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome

Miller

Truong

Barton

et al. 2014

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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The composition of the typical commercial diet fed to zebrafish can dramatically vary. By utilizing defined diets we sought to answer two questions: 1) How does the embryonic zebrafish transcriptome change when the parental adults are fed a commercial lab diet compared with a sufficient, defined diet (E+)? 2) Does a vitamin Edeficient parental diet (E−) further change the embryonic transcriptome? We conducted a global gene expression study using embryos from zebrafish fed a commercial (Lab), an E+ or an E− diet. To capture differentially expressed transcripts prior to onset of overt malformations observed in E− embryos at 48 h post-fertilization (hpf), embryos were collected from each group at 36 hpf. Lab embryos differentially expressed (p < 0.01) 946 transcripts compared with the E+ embryos, and 2656 transcripts compared with the E− embryos. The differences in protein, fat and micronutrient intakes in zebrafish fed the Lab compared with the E + diet demonstrate that despite overt morphologic consistency, significant differences in gene expression occurred. Moreover, functional analysis of the significant transcripts in the E−embryos suggested perturbed energy metabolism, leading to overt malformations and mortality. Thus, these findings demonstrate that parental zebrafish diet has a direct impact on the embryonic transcriptome.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome

Miller

Truong

Barton

et al. 2014

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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“…Cells were scraped then spun down for 15 min at 13,000 ϫ g at 4°C, and the supernatant was analyzed by LC-MS/MS. Metabolic profiling was performed as previously described, except column temperature was held at 50°C (24). Importantly, the metabolite identification process was rigorous.…”

Section: Methodsmentioning

confidence: 99%

A Metabolomics-driven Elucidation of the Anti-obesity Mechanisms of Xanthohumol

Kirkwood

Legette²,

Miranda³

et al. 2013

Journal of Biological Chemistry

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“…(Hung et al, 2012; Santoro, 2014). Recent studies suggest that zebrafish may be an ideal reference model system for performing metabolomic-related studies (Kirkwood et al, 2012; Seth et al, 2013; Santoro, 2014). Furthermore, the metabolic changes in zebrafish are conserved in human samples (Nath et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish

Elie

Choi

Nkrumah‐Elie

et al. 2015

Environmental Research

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Polycyclic aromatic hydrocarbons (PAHs) and their oxygenated derivatives are ubiquitously present in diesel exhaust, atmospheric particulate matter and soils sampled in urban areas. Therefore, inhalation or non-dietary ingestion of both PAHs and oxy-PAHs are major routes of exposure for people; especially young children living in these localities. While there has been extensive research on the parent PAHs, limited studies exist on the biological effects of oxy-PAHs which have been shown to be more soluble and more mobile in the environment. Additionally, investigations comparing the metabolic responses resulting from parent PAHs and oxy-PAHs exposures have not been reported. To address these current gaps, an untargeted metabolomics approach was conducted to examine the in vivo metabolomic profiles of developing zebrafish (Danio rerio) exposed to 4 µM of benz[a]anthracene (BAA) or benz[a]anthracene-7, 12-dione (BAQ). By integrating multivariate, univariate and pathway analyses, a total of 62 metabolites were significantly altered after 5 days of exposure. The marked perturbations revealed that both BAA and BAQ affect protein biosynthesis, mitochondrial function, neural development, vascular development and cardiac function. Our previous transcriptomic and genomic data were incorporated in this metabolomics study to provide a more comprehensive view of the relationship between PAH and oxy-PAH exposures on vertebrate development.

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Vitamin C Deficiency Activates the Purine Nucleotide Cycle in Zebrafish

Cited by 60 publications

References 41 publications

The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome

The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome

A Metabolomics-driven Elucidation of the Anti-obesity Mechanisms of Xanthohumol

Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish

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