Vitamin D intestinal absorption is not a simple passive diffusion: Evidences for involvement of cholesterol transporters

Reboul, Emmanuelle; Goncalves, Aurélie; Coméra, Christine; Bott, Raoul; Nowicki, Marion; Landrier, Jean‐François; Jourdheuil-Rahmani, Dominique; Dufour, Claire; Collet, Xavier; Borel, Patrick

doi:10.1002/mnfr.201000553

Cited by 176 publications

(200 citation statements)

References 51 publications

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“…The molecular mechanisms involved in VD uptake/ secretion by adipose tissue have not yet been investigated but may well involve the megalin/cubulin pathway (described later) as suggested by Abboud et al (25) and/ or cholesterol transporters as described in the intestine (17) or for other lipophilic micronutrients in adipose tissue (26) . (27) .…”

Section: Uptake Of Vitamin Dmentioning

confidence: 99%

Vitamin D modulates adipose tissue biology: possible consequences for obesity?

et al. 2015

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Cross-sectional studies depict an inverse relationship between vitamin D (VD) status reflected by plasma 25-hydroxy-vitamin D and obesity. Furthermore, recent studies in vitro and in animal models tend to demonstrate an impact of VD and VD receptor on adipose tissue and adipocyte biology, pointing to at least a part-causal role of VD insufficiency in obesity and associated physiopathological disorders such as adipose tissue inflammation and subsequent insulin resistance. However, clinical and genetic studies are far less convincing, with highly contrasted results ruling out solid conclusions for the moment. Nevertheless, prospective studies provide interesting data supporting the hypothesis of a preventive role of VD in onset of obesity. The aim of this review is to summarise the available data on relationships between VD, adipose tissue/adipocyte physiology, and obesity in order to reveal the next key points that need to be addressed before we can gain deeper insight into the controversial VD-obesity relationship.Adipose tissue: Vitamin D: Obesity: Inflammation: Adipocytes: Nutrients: Nutrition Vitamin D: a brief overview Vitamin D (VD; calciferol) is a hormone mainly described for its role as a regulator of phosphate and calcium homeostasis (1) . It can be obtained through animal (VD 3 , cholecalciferol) or plant (VD 2 , ergocalciferol) food sources. Only a few foodstuffs contain significant amounts of VD, the main sources being fish liver oils, fatty fish (sardines, herring and mackerel) and egg yolk (2,3) , but small quantities are also found in fortified milk, orange juice, bread and cereals. Alternatively, VD 3 is produced endogenously in the skin after UVB irradiation from the precursor 7-dehydrocholesterol to give pre-VD 3 , which is further isomerised to VD 3 before being released into the circulation (4) . Classical estimates have assigned a majority (70-90 %) of VD supply to dermal synthesis, but a recent paper revised this figure down to just 10-25 % of VD supply (5) and posited that dietary intake of 25-hydroxy-vitamin D (25(OH)D) is a significant contributor to total VD input.Adipose tissue is a major storage site for vitamin D Despite limited data, it is widely accepted that adipose tissue is a reservoir for VD in human subjects and rats (6)(7)(8)(9)(10) . Interestingly, visceral fat was found to contain 20 % more VD than subcutaneous fat (11) . Heaney et al. (12) calculated that 65 % of total VD in the body is in the form of D 3 , for which adipose tissue and skeletal muscle appear to be the main body stores (accounting for 73 and 16 %, respectively).

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Section: Uptake Of Vitamin Dmentioning

confidence: 99%

Vitamin D modulates adipose tissue biology: possible consequences for obesity?

et al. 2015

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“…This is mainly due to the effect of the plant matrix, which can impair the release of some vitamins in the gut and thus diminish their absorption (Castenmiller et al, 1999;Borel, 2003). It should also be considered that some vitamins compete for absorption in the human body, for example, vitamin E and lutein (Reboul et al, 2006a), and vitamin E and vitamin D (Reboul et al, 2011).…”

Section: Considerations For Engineering Vitamin-biofortified Cropsmentioning

confidence: 99%

Vitamin Deficiencies in Humans: Can Plant Science Help?

et al. 2012

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The term vitamin describes a small group of organic compounds that are absolutely required in the human diet. Although for the most part, dependency criteria are met in developed countries through balanced diets, this is not the case for the five billion people in developing countries who depend predominantly on a single staple crop for survival. Thus, providing a more balanced vitamin intake from high-quality food remains one of the grandest challenges for global human nutrition in the coming decade(s). Here, we describe the known importance of vitamins in human health and current knowledge on their metabolism in plants. Deficits in developing countries are a combined consequence of a paucity of specific vitamins in major food staple crops, losses during crop processing, and/or overreliance on a single species as a primary food source. We discuss the role that plant science can play in addressing this problem and review successful engineering of vitamin pathways. We conclude that while considerable advances have been made in understanding vitamin metabolic pathways in plants, more cross-disciplinary approaches must be adopted to provide adequate levels of all vitamins in the major staple crops to eradicate vitamin deficiencies from the global population.

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“…Caco-2 clone TC-7 cells were cultured in the presence of DMEM supplemented with 16% heat-inactivated FBS, 1% nonessential aa, and 1% antibiotics (complete medium), as previously described ( 26 ). Cells were seeded and grown on transwells for 21 days, as previously described ( 26 ), to obtain confl uent and highly-differentiated cell monolayers.…”

Section: Caco-2 Cell Culturementioning

confidence: 99%

“…Cells were seeded and grown on transwells for 21 days, as previously described ( 26 ), to obtain confl uent and highly-differentiated cell monolayers. At 12 h prior to each experiment, the medium used in apical and basolateral chambers was a serum-free complete medium.…”

Section: Caco-2 Cell Culturementioning

confidence: 99%

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Micellar lipid composition affects micelle interaction with class B scavenger receptor extracellular loops

Goncalves

Gontero

Nowicki

et al. 2015

Journal of Lipid Research

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International audienceScavenger receptors (SRs) like cluster determinant 36 (CD36) and SR class B type I (SR-BI) play a debated role in lipid transport across the intestinal brush border membrane. We used surface plasmon resonance to analyze real-time interactions between the extracellular protein loops and various ligands ranging from single lipid molecules to mixed micelles. Micelles mimicking physiological structures were necessary for optimal binding to both the extracellular loop of CD36 (lCD36) and the extracellular loop of SR-BI (lSR-BI). Cholesterol, phospholipid, and fatty acid micellar content significantly modulated micelle binding to and dissociation from the transporters. In particular, high phospholipid micellar concentrations inhibited micelle binding to both receptors (−53.8 and −74.4% binding at 0.32 mM compared with 0.04 mM for lCD36 and lSR-BI, respectively, P < 0.05). The presence of fatty acids was crucial for micelle interactions with both proteins (94.4 and 81.3% binding with oleic acid for lCD36 and lSR-BI, respectively, P < 0.05) and fatty acid type substitution within the micelles was the component that most impacted micelle binding to the transporters. These effects were partly due to subsequent modifications in micellar size and surface electric charge, and could be correlated to micellar vitamin D uptake by Caco-2 cells. Our findings show for the first time that micellar lipid composition and micellar properties are key factors governing micelle interactions with SRs

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Vitamin D intestinal absorption is not a simple passive diffusion: Evidences for involvement of cholesterol transporters

Abstract: These data show for the first time that vitamin D intestinal absorption is not passive only but involves, at least partly, some cholesterol transporters.

Cited by 176 publications

References 51 publications

Vitamin D modulates adipose tissue biology: possible consequences for obesity?

Vitamin D modulates adipose tissue biology: possible consequences for obesity?

Vitamin Deficiencies in Humans: Can Plant Science Help?

Micellar lipid composition affects micelle interaction with class B scavenger receptor extracellular loops

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