2020
DOI: 10.3390/antiox9090838
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Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells

Abstract: Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current study, we investigated the role of vitamin D against oxidative stress and inflammation in human retinal pigment epithelium (RPE) and human retinal endothelial cell lines. We demonstrate that vitamin D effectively coun… Show more

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Cited by 42 publications
(58 citation statements)
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References 76 publications
(102 reference statements)
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“…The same cell lines and culture system were used as previously described [ 36 ]. Briefly, human retinal pigment epithelial cells, ARPE-19 (CRL-2302, ATCC, Manassas, VG, USA; 3 passages) were grown to confluence in a standard incubator in DMEM (D6429, Sigma-Aldrich, St Louis, MO, USA) containing 10% fetal bovine serum (FBS; 10270106 Gibco TM ThermoFisher, Paisley, UK), 1% fungizone (Gibco TM ), and penicillin–streptomycin (Gibco TM ).…”
Section: Methodsmentioning
confidence: 99%
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“…The same cell lines and culture system were used as previously described [ 36 ]. Briefly, human retinal pigment epithelial cells, ARPE-19 (CRL-2302, ATCC, Manassas, VG, USA; 3 passages) were grown to confluence in a standard incubator in DMEM (D6429, Sigma-Aldrich, St Louis, MO, USA) containing 10% fetal bovine serum (FBS; 10270106 Gibco TM ThermoFisher, Paisley, UK), 1% fungizone (Gibco TM ), and penicillin–streptomycin (Gibco TM ).…”
Section: Methodsmentioning
confidence: 99%
“…As previously described [ 36 ], immunofluorescence with RPE65 (1:100, 78036, Abcam, Cambridge, USA) and caveolin (1:250, 3238S, Cell Signalling, Danvers, MA, USA) was performed for ARPE-19 and HREC cells, respectively. Briefly, 100,000 ARPE-19 and 50,000 HREC cells were seeded on a 10 mm dish (Menzel-Glaser, Waltham, MA, USA) and following cellular fixation with cold methanol, cells were blocked with 1% BSA, 0.5% Triton X-100, 0.2% sodium azide, and 1% FBS for 1 h at 4 °C.…”
Section: Methodsmentioning
confidence: 99%
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“…The first five manuscripts discuss in vitro approximations. Fernández-Robredo et al report results showing the antioxidant and anti-inflammatory properties of vitamin D, suggesting its usefulness in moderating the chronic low-grade inflammation and oxidative stress in the development of DR [ 15 ]. Oh et al show the antioxidant capacity of two supplements, ascorbic acid and astaxanthin, using two different oxidative models on a human retinal pigment epithelia cell line (ARPE-19) [ 16 ].…”
mentioning
confidence: 99%