Vitamin E ameliorates oxidative stress in the stomach of l-arginine induced acute pancreatitis rat model

Abstract

Gut dysfunction is a major outcome during acute pancreatitis (AP). Some studies have reported a positive association between acute pancreatitis and peptic ulcer. The major cause of peptic ulcer is due to ischaemia reperfusion leading to oxidative stress. This study assessed the oxidative status of the stomach during AP and the role of vitamin (Vit.) E administration. Twenty male Wistar rats were divided randomly into 4 groups; Control, Vit. E (500 mg/kg) only, AP and Vit. E + AP. Acute pancreatitis was induced by administering four doses of l-arginine (100 mg/100 g) intraperitoneally at 1-hour interval. The rats were sacrificed 72 hours later and stomach obtained for mucin content and biochemical assays (MDA, MPO, NO, CAT, GST, and GSH). Mucin content was depleted following AP induction while, vit. E significantly increase mucin content. AP resulted in significant reduction of antioxidant enzymes (GSH and GST) and increase in oxidative stress markers (MDA, MPO and NO). Vitamin E reverse these changes. In conclusion, the oxidative status of the stomach was greatly altered following AP induction with an increase in oxidative stress markers and decrease in antioxidants. Vitamin E was able to protect the stomach from the damaging effect of acute pancreatitis.