Vitamin E dependent microRNA regulation in rat liver

Gaedicke, Sonja; Zhang, Xiangnan; Schmelzer, Constance; Lou, Yijia; Doering, Frank; Frank, Jan; Rimbach, Gerald

doi:10.1016/j.febslet.2008.09.032

Cited by 106 publications

(93 citation statements)

References 37 publications

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“…Real-time quantitative PCR was performed as a one-step procedure (SensiMixTM One-step Kit; Quantace, Berlin, Germany) with SybrGreen detection, using the Rotorgene 6000 cycler (Corbett Life Science, Sydney, NSW, Australia) (11) . Quantification was done using a standard curve.…”

Section: Rna Isolation and Real-time Quantitative Rt-pcrmentioning

confidence: 99%

Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

et al. 2010

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Paraoxonase 1 (PON1) is an enzyme that is mainly synthesised in the liver and protects LDL from oxidation, thereby exhibiting antiatherogenic properties. Using a luciferase reporter gene assay, we tested curcumin for its ability to induce PON1 in Huh7 hepatocytes in culture. Curcumin ($10 mmol/l) dose-dependently induced PON1 transactivation in Huh7 cells. However, dietary supplementation of female B6C3F1 mice with curcumin (500 mg/kg diet) for 2 weeks did not increase the hepatic PON1 mRNA and protein levels. No curcumin was detectable in the plasma of the 12 h fasted mice. In conclusion, curcumin may be a potent PON1 inducer in cultured cells in vitro, but not in the liver of curcumin-fed mice because of its low concentrations in vivo.

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Section: Rna Isolation and Real-time Quantitative Rt-pcrmentioning

confidence: 99%

Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

et al. 2010

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“…This indicates a pathway for targeted therapy via VDR downregulation in human malignancies [58]. Vitamin E modulates lipid metabolism, inflammation, and other cancer-associated pathways by altering the expression of miR-122 and miR-125b [59].…”

Section: Mirna Associated Epigenetic Regulation Mediated By Dietmentioning

confidence: 98%

Effect Of Diet and Nutrients on Molecular Mechanism of Gene Expression Mediated By Nuclear Receptor and Epigenetic Modulation

Jalili¹

2013

TONUTRAJ

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Abstract:Major research progress in the last few decades has elucidated the complex nexus between nutrition and health. Diet and lifestyle influence epigenetic changes that are heritable. However, a statement of reservation is needed here, viz. that it is often difficult to distinguish between epigenetic changes that are inherited from one generation to the next and true mutations, for instance in mitochondrial DNA. The last topic is a big one in its own right and will not be further discussed in this article. Epigenetic changes induced by dietary nutrients ultimately culminate in changes of the expression of genes through transcription and translation. The interaction between dietary nutrients and nuclear receptors triggers the signaling pathway, leading to modulation of epigenetic change and gene expression. Knowledge about nuclear receptors is important for explaining dietary modulation of transcription via recruitment of large protein complexes. These proteins are capable of causing modification of chromosomal components, can influence chromatin proteins, and affect the binding of proteins to particular parts of the DNA molecules controlling the expression of individual genes. Chromatin complexes between DNA and proteins can be destabilized by recruitment of transcriptional coactivators by histone acetylation. However, in the presence of hormone antagonists or in the absence of relevant ligands, recruitment of other cellular core proteins may stabilize chromatin by their influence on histone deacetylases, thus antagonizing the effect of enzymes causing histone acetylation. This article reviews the current knowledge on nutritional modulation of bioactive molecules by epigenetic changes, if they can regulate genetic expressions. The molecular mechanism of action of various dietary nutrients on gene expression mediated by nuclear receptors is also discussed.

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“…On the contrary, vitamin E deficiency in rats decreased tumor suppressor miRNAs in the liver. Vitamin E has also has been reported to modulate lipid metabolism, inflammation, and other cancer-associated pathways by reducing the expression of miR-122 and miR-125b [127] . Vitamin D has long been considered as an essential nutrient for human health and evidence from epidemiologic and experimental studies have indicated that higher intakes of vitamin D from food and/or supplements leads to higher blood levels of vitamin D, which are highly associated with reduced risks of certain cancers [128,129] .…”

Section: Role Of Nutritive Natural Agents In the Regulation Of Mirnasmentioning

confidence: 99%

Modulation of miRNAs by Natural Agents: Nature’s way of dealing with cancer

Masika

Zhao

Hescheler

et al. 2015

RNA Dis

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Accumulating lines of evidence have revealed that microRNAs (miRNAs) play critical roles in many biological processes, such as carcinogenesis, angiogenesis, programmed cell death, cell proliferation, invasion, migration, and differentiation. They act either as tumor suppressors or oncogenes, and alteration in their expression patterns has been linked to onset, progression and chemoresistance of various cancers. Moreover, miRNAs are also crucial for the regulation of cancer stem cells (CSCs) self-renewal and proliferation as well as control of Epithelial-to-Mesenchymal Transition (EMT) of cancer cells. Therefore, exploitation of miRNAs as targets for cancer prevention and therapy could be a promising approach. Several experimental and epidemiologic studies have shown that dietary intake of natural agents such as baicalin, ginsenoside, curcumin, resveratrol, genistein, epigallocatechin-3-gallate (EGCG), indole-3-carbinol, 3,3΄-diindolylmethane (DIM) including antioxidants among others is inversely associated with the risk for cancer, demonstrating the inhibitory effects of natural agents on carcinogenesis. Additionally, the anticancer agents from natural agents have been found to inhibit the development and progression of cancer through the regulation of various cancer processes such as apoptosis, cell cycle regulation, differentiation, inflammation, angiogenesis, and metastasis. Importantly, natural agents could significantly impact the expression of tumor-suppressor and oncogenic miRNAs, regulate cellular signaling networks, inhibit cancer cell growth and cancer stem cell self-renewal. This is important for the normalization of altered cellular signaling mechanisms in cancer cells. This postulates a much broader use of natural agents in the prevention and/or treatment of various cancers in combination with conventional chemotherapeutics. However, more in vitro mechanistic experiments, in vivo animal studies, and clinical trials are warranted to realize the true value of natural agents in the prevention and/or treatment of cancer. Herein, we provide an overview of natural agents' modulation of miRNA expression as well as highlight the significance of these observations as potential new strategies in cancer therapies. This review will help us to understand how miRNAs are regulated by natural agents and also help in the development of effective and secure natural agents for therapeutic purposes.

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Vitamin E dependent microRNA regulation in rat liver

Cited by 106 publications

References 37 publications

Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

Effect Of Diet and Nutrients on Molecular Mechanism of Gene Expression Mediated By Nuclear Receptor and Epigenetic Modulation

Modulation of miRNAs by Natural Agents: Nature’s way of dealing with cancer

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