Vitamin E (Trolox) addition to Tris-egg yolk extender preserves ram spermatozoon structure and kinematics after cryopreservation

Silva, Sildivane Valcácia; Soares, Adriana Trindade; Batista, André Mariano; Almeida, Felipe Costa; Nunes, J. F.; Peixoto, Christina Alves; Guerra, Maria Madalena Pessoa

doi:10.1016/j.anireprosci.2012.12.002

Cited by 48 publications

(39 citation statements)

References 35 publications

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“…It was clear that ROS accumulated during the cooling, equilibration, freeze-thawing and postthaw incubation of sperm. Our results showed that α-tocopherol has suitable cryo-protective effects through its ability to quench ROS accumulation, which is in agreement with several studies that stated analogous of vitamin E in extenders increased the recovery rate of motility and viability of sperm (Donoghue & Donoghue, 1997;Surai et al, 1998;Silva et al, 2013). Moreover, similar to our study, Domínguez-Rebolledo et al (2010) reported that motion characteristics and acrosomal integrity of epididymal red deer spermatozoa were improved when Trolox was added to incubation medium after thawing.…”

Section: Discussionsupporting

confidence: 92%

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Motemani

Chamani

Sharafi

et al. 2017

Span. j. agric. res.

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This study was conducted to investigate the effects of different levels of α-tocopherol in cryopreservation media on the bull sperm quality after thawing. Semen samples were collected from six Holstein bulls using artificial vagina twice a week. Semen samples were pooled to eliminate individual differences and then divided into four equal parts for freezing with extenders containing different concentrations of α-tocopherol according to experimental groups as follows: 0 (control), 1.2 mM (E1), 2.4 mM (E2) and 4.8 mM (E4). Motion characteristics, viability, plasma membrane functionality, lipid peroxidation and H 2 O 2 status were determined after thawing. Results showed that malondialdehyde (MDA) concentration was significantly lower in E4 (6.1±0.6 nmol/mL) than E1 and E2 extenders (8.1±0.6 nmol/mL and 8±0.6 nmol/mL, respectively). Also, the lowest significant concentration of H 2 O 2 was observed in E4 (3.2±0.13 nmol/mL) compared to E1 (4 ±0.1 nmol/mL), E2 (5.3± 0.1 nmol/mL) and control (6.7±0.1 nmol/mL). Moreover, E2 and E4 produced the highest significant motility (74.2±1.6%, 75.9±1.6%), viability (78.2±1.8%, 76.1±1.8%) and membrane functionality (73±1.6%, 70.5±1.6%) compared to other groups. It can be concluded that α-tocopherol at the concentration of 4.8 mM can be an efficient antioxidant additive in Bioxcell extender for cryopreservation of bull semen.Additional key words: antioxidant; bioxcell; lipid peroxidation; sperm; H 2 O 2 .Correspondence should be addressed to Mohammad Chamani: m.chamani@srbiau.ac.ir Abbreviations used: LPO (lipid peroxidation); MDA (malondialdehyde); ROS (reactive oxygen species).Funding: The authors received no specific funding for this work.

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Section: Discussionsupporting

confidence: 92%

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Motemani

Chamani

Sharafi

et al. 2017

Span. j. agric. res.

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“…Likewise, Mata-Campuzano et al (2015) indicated that the supplementation of Trolox, a water-soluble vitamin E analog, in a TESTris-fructose extender reduces lipid peroxidation after thawing of frozen ram semen. Silva et al (2013) reinforced the previous finding, reporting that Trolox addition to Tris-egg yolk extender at 60 and 120μM provides higher plasma membrane and mitochondria integrity, as well as, better kinematics for ram cryopreserved spermatozoa. These results confirm also Maia's et al (2007; observations that Trolox addition at 50-100 μM to freezing extender resulted in increased semen motility.…”

Section: Discussionsupporting

confidence: 89%

Current status and advances in ram semen cryopreservation

Ntemka

Tsakmakidis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

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Ram semen cryopreservation contributes to genetic improvement through artificial insemination, eliminates geographical barriers in artificial insemination application and supports the preservation of endangered breeds thus the conservation of biodiversity. Sperm freezing process induces ultrastructural, biochemical and functional changes of spermatozoa. Especially, spermatozoa’s membranes and chromatin can be damaged, sperm membranes’ permeability is increased, hyper oxidation and formation of reactive oxygen species takes place, affecting fertilizing ability and subsequent early embryonic development. Aiming to improve ram frozen-thawed semen’s fertilizing capacity, many scientific investigations took place. Among them the composition of semen extenders, was a main point of interest. Semen preservation extenders regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cryogenic stress. Therefore, permeating (glycerol, dimethyl sulfoxide) and non-permeat ing (egg yolk, skimmed milk) cryoprotectants, sugars (glucose, lactose, trehalose, raffinose), salts (sodium citrate, citric acid) and antioxidants (amino acids, vitamins, enzymes) have been added and tested. Moreover, semen dilution rate, storage temperature, cooling rate and thawing protocol, are also some key factors that have been studied. The research results of this scientific topic are encouraging, not only about the freezing and thawing procedures, but also about the improvement of the additives’ properties. However, further research is needed to enhance the fertilizing ability of ram frozen-thawed semen, making its use practical in sheep reproductive management by the application of cervical artificial insemination.

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“…In the current study, E5 extender resulted in a significant increase in TM and PM values. It has been demonstrated that vitamin E supplemented extender improves the motility of sperm in different species (Donoghue and Donoghue1997;Surai et al 1998;Breininger et al 2005;Asadpour et al 2012;Silva et al 2013). It seems that vitamin C and E-supplemented semen extender (C200 and E5) result in decreased MDA production and consequently improved sperm motility.…”

Section: Discussionmentioning

confidence: 99%

“…In recent studies, the supplementation of freezing diluents with vitamin E has been improved the post-thawed sperm functions of ram (Silva et al 2013), sea bass (Martinez-Paramo et al 2012), boar (Breininger et al 2005) and cat (Thuwanut et al 2008). Also, the supplementation of freezing diluents with vitamin E resulted in a positive effect on sperm motility, mitochondrial membrane potential and integrity of the sperm membrane and DNA (Thuwanut et al 2008;Jeong et al 2009).…”

Section: Introductionmentioning

confidence: 99%

The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality

et al. 2015

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Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reason of declined motility and fertility of sperm during the freeze-thawing process. This study was conducted to determine the influence of vitamin C and vitamin E on rooster post-thawed sperm motility, viability and malondialdehyde (MDA) level. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing with no antioxidants (control), or containing 100 (C100), 200 (C200), 400 (C400), 800 (C800) µg/mL vitamin C, and 2 (E2), 5 (E5), 10 (E10) and 15 (E15) µg/mL vitamin E. After thawing, total and progressive sperm motility, sperm viability and semen MDA level were assessed. The results shown that C200 and E5 extenders resulted in higher total motility (p < 0.05) compared to other extenders, with exception of E10 extender. Progressive motility was higher in E5 extender (p < 0.05) compared to other extenders, with exception of C200 and E10 extenders. Also, C200 and E5 extenders resulted in higher viability of post-thawed spermatozoa (p < 0.05) compared to other extenders. Finally, the results showed that MDA level was lower in C100 and C200 extenders compared to other extenders (p < 0.05), with exception of E5 extender. In conclusion, the results of the present study demonstrate that C200 and E5 can improve the function of post-thawed rooster spermatozoa.

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Vitamin E (Trolox) addition to Tris-egg yolk extender preserves ram spermatozoon structure and kinematics after cryopreservation

Cited by 48 publications

References 35 publications

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Current status and advances in ram semen cryopreservation

The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality

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