1941
DOI: 10.1016/s0021-9258(19)56172-5
|View full text |Cite
|
Sign up to set email alerts
|

Vitamin K Activity and Structure

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

0
4
0

Year Published

1942
1942
2016
2016

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 79 publications
(4 citation statements)
references
References 48 publications
0
4
0
Order By: Relevance
“…VKOR activity was determined by measuring the level of KO reduction. KO was prepared by oxidation of phylloquinone (Sigma), as described previously (21), and then purified by HPLC using a preparative C18 column (Vydac) and isocratic elution with methanol. Lysates (400 µL, ∼1-2 mg) prepared as described previously (22) were incubated with this KO preparation (giving final concentrations of 15 µM KO and 1% methanol) for 1 min, followed by the addition of DTT (to 5 mM) and incubation for an additional 20 min, all at 20 °C.…”
Section: Methodsmentioning
confidence: 99%
“…VKOR activity was determined by measuring the level of KO reduction. KO was prepared by oxidation of phylloquinone (Sigma), as described previously (21), and then purified by HPLC using a preparative C18 column (Vydac) and isocratic elution with methanol. Lysates (400 µL, ∼1-2 mg) prepared as described previously (22) were incubated with this KO preparation (giving final concentrations of 15 µM KO and 1% methanol) for 1 min, followed by the addition of DTT (to 5 mM) and incubation for an additional 20 min, all at 20 °C.…”
Section: Methodsmentioning
confidence: 99%
“…Whatman KC-18F plates were prewashed in chloroform, developed with 99 mol % acetonitrile, and eluted with chloroform. Chemical formation of vitamin K 2,3-epoxide was carried out as described by Fieser et al (1941). Radioactive vitamin K epoxide was purified by the same thin-layer chromatographic procedure as was used for the tritiated vitamin.…”
Section: Methodsmentioning
confidence: 99%
“…Vitamin K epoxidase was assayed by using tritiated vitamin Kl with conversion to vitamin K, epoxide determined by reversed-phase thin-layer chromatography (Willingham & Matschiner, 1974;Friedman & Smith, 1977). To study the epoxide reductase, we converted tritiated vitamin K, to tritiated vitamin K, epoxide by using the method of Fieser et al (1941); after incubation of the epoxide with microsomes, conversion of epoxide to the quinone form of the vitamin was measured by reversed-phase thin-layer chromatography (Zimmerman & Matschiner, 1974).…”
Section: Methodsmentioning
confidence: 99%