Vitellogenin in Winter Flounder (Pleuronectes americanus) from Long Island Sound and Boston Harbor

Pereira, Jose J.; Ziskowski, John J.; Mercaldo‐Allen, Renée; Kuropat, Catherine; Luedke, Dan W.; Gould, Edith

doi:10.2307/1352777

Cited by 38 publications

(11 citation statements)

References 36 publications

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“…PAHs, PCBs (Janssen et al 1996), cadmium (Pereira et al 1992), crude oil (Truscott et al 1992). They are also consistent with increasing levels of endogenous oestrogens in female fish during vtg synthesis (Janssen et al 1995) and exposure to exogenous oestrogens in male fish, since oestrogen raises the amount of endoplasmatic reticulum in the liver, thus indicating enhanced protein (vtg) synthesis (Emmersen & Emmersen 1976).…”

Section: S Ti T2 T3mentioning

confidence: 61%

Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent

Lye¹,

Frid²,

Gill³

1998

Mar. Ecol. Prog. Ser.

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ABSTRACT. Indicators of environmental oestrogen exposure in male and female wild flounder Platichthys flesus were followed over the annual reproductive cycle. Fish were obtained from the Tyne estuary, UK, which receives effluent from a large sewage treatment works and several other anthrophogenic sources and the Solway Firth, UK, which receives only low levels of sewage effluent. Vitellogenin (vtg), a female-spec~fic protein, was present in the plasma of up to 9 4 % of males from the Tyne and was accompanied by inhibited spermatogenesis and the incidence of pathological testic'ular abnormalities. The vtg levels in males are independent of the seasonal cycle. Female flounders from the Tyne exhibited increased vtg levels and a higher proportion of degenerating oocytes than Solway Firth females. This study provides limited evidence that wild populations of flounders showing oestrogenic responses are also suffering reproductive disturbances but the degree of alteration varies during the seasonal reproductive cycle.

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Section: S Ti T2 T3mentioning

confidence: 61%

Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent

Lye¹,

Frid²,

Gill³

1998

Mar. Ecol. Prog. Ser.

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“…Pereira et al (1992) detected lower vitellogenin levels in female winter flounder (Pleuronectes americanus) in an area of Boston Harbor contaminated with PAHs and other contaminants. Vitellogenin production in female F. heteroclitus was also negatively correlated with sediment PAHs in the spring of both years.…”

Section: Plasma Vitellogenin and Contaminantsmentioning

confidence: 92%

A survey of indicators for reproductive endocrine disruption in Fundulus heteroclitus (killifish) at selected sites in the Chesapeake Bay

Pait

Nelson

2009

Marine Environmental Research

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Plasma vitellogenin and related parameters in the killifish Fundulus heteroclitus were measured at selected sites in the Chesapeake Bay. In males, vitellogenin was above the detection limit 14% of the time, and detections did not differ between sites or seasons. Few differences in plasma vitellogenin levels were found between sites during fall in either male or female F. heteroclitus, the time of natural gonadal regression for this species. There was some variation in the ratio of male to female F. heteroclitus, but was not consistent at most sites. Significant negative correlations were found between reported sediment polycyclic aromatic hydrocarbons (PAHs) and GSI, and PAHs and plasma vitellogenin in females in both Spring 1999 and Spring 2000. Gonadal anomalies in F. heteroclitus included slight reductions in certain tissue types. Overall, reproductive endocrine disruption in the killifish F. heteroclitus at the sites sampled in the Chesapeake Bay appeared somewhat minimal.

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“…VTG can be measured via methods such as radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), immunodiffusion, and alkali-labile phosphate [9]. …”

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-linked Immunosorbent Assay Using Vitellin for Vitellogenin Measurement in the Pale Chub, <i>Zacco platypus</i>

Lim

Lee

Kim

et al. 2013

Environ Health Toxicol

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ObjectivesFish vitellogenin (VTG) is produced in the female liver during oogenesis through the estradiol cycle and produced in the male liver by endocrine disrupting chemicals (EDCs) such as alkylphenols. In this study, we propose that the VTG concentration in the pale chub could be detected using monoclonal antibodies and polyclonal antibodies against vitellin (Vn) in a VTG enzyme-linked immunosorbent assay (ELISA) system.MethodsMonoclonal antibodies and polyclonal antibodies were produced using the Vn extracted from the matured ovum of the ovary. The VTG was extracted from the plasma of the male pale chub. The Vn and VTG were confirmed by measuring the molecular weight of their proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the specificity of the antibodies was checked through western blotting methods. The assay system was validated with respect to optimal assay concentrations, specificity, recovery, and intra- and inter-assay variations.ResultsThe Vn consisted of two protein bands with apparent molecular weights of 64 and 37 kDa. The SDS-PAGE indicated protein weights of 146 and 77 kDa in the VTG. The assay range was 15.6 ng/mL to 2,000 ng/mL, and the value of the intra- and inter-assay variations were within 10.0% and 14.7%, respectively. The recovery rate was 99.5±5.5%.ConclusionsA sandwich ELISA was developed that could be used to qualify the VTG of pale chub in screening for EDCs. Pale chub is an ideal species for observing estrogen activity in the environment because of its extensive habitat and extensive food chain. The ELISA developed here would be more favorable than those for other species for determining the effect of long-term food chain accumulation of EDCs in aquatic environments.

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Vitellogenin in Winter Flounder (Pleuronectes americanus) from Long Island Sound and Boston Harbor

Cited by 38 publications

References 36 publications

Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent

Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent

A survey of indicators for reproductive endocrine disruption in Fundulus heteroclitus (killifish) at selected sites in the Chesapeake Bay

Development of an Enzyme-linked Immunosorbent Assay Using Vitellin for Vitellogenin Measurement in the Pale Chub, <i>Zacco platypus</i>

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Vitellogenin in Winter Flounder (Pleuronectes americanus) from Long Island Sound and Boston Harbor

Cited by 38 publications

References 36 publications

Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent

Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent

A survey of indicators for reproductive endocrine disruption in Fundulus heteroclitus (killifish) at selected sites in the Chesapeake Bay

Development of an Enzyme-linked Immunosorbent Assay Using Vitellin for Vitellogenin Measurement in the Pale Chub, &lt;i&gt;Zacco platypus&lt;/i&gt;

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Development of an Enzyme-linked Immunosorbent Assay Using Vitellin for Vitellogenin Measurement in the Pale Chub, <i>Zacco platypus</i>