Vitrification and in-straw warming do not affect pregnancy rates of biopsied bovine embryos

González-Rodriguez, Núria; Martínez-Rodero, Iris; Jung, Simone; Reichenbach, M.; Zablotski, Yury; Otzdorff, Christiane; Zerbe, Holm; Mogas, T.

doi:10.1016/j.theriogenology.2022.07.021

Cited by 6 publications

(1 citation statement)

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“…Embryo biopsy does not affect cryosurvival. Many studies in mouse and human embryos have demonstrated similar results, and biopsy coupled with vitrification has been followed in medical practice aiming to perform PGT before embryo transfer in human patients (Griffin and Ogur, 2018) Studies with bovine embryos show similar results, with no effect of biopsy on cryosurvival (González-Rodríguez et al, 2022;Najafzadeh et al, 2021;Takahashi et al, 2013;Tominaga, 2004), even though cryopreservation generally decreased pregnancy rates in bovine embryos, especially observed in earlier studies (Tominaga, 2004). Since cryopreservation protocols have been largely updated in bovine embryology during the past decades, storage of frozen-biopsied embryo while genotyping is performed, and breeding value is calculated is a good choice.…”

Section: Technical Aspects Of Embryo Biopsymentioning

confidence: 89%

Embryo biopsies for genomic selection in tropical dairy cattle

et al. 2023

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Genomic selection has transformed the livestock industry, enabling early-life selection of animals. Biopsy sampling of pre-implantation embryos has been described since 1968. However, it was only after 2010, with the advancement of molecular biology techniques such as whole genomic amplification and SNP Chips, that next-generation sequencing became commercially available for bovine embryos. It is now possible to make decisions about which embryos to transfer not only based on recipients' availability or embryo morphology but also on genomic estimates. This technology can be implemented for a wide spectrum of applications in livestock. In this review, we discuss the use of embryo biopsy for genomic selection and share our experience with Gir and Girolando Brazilian breeding programs, as well as future goals for implementing it in Brazilian bovine in vitro embryo production practices.

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