Vitrification and transfer of cynomolgus monkey (Macaca fascicularis) embryos fertilized by intracytoplasmic sperm injection

Yamasaki, Junichi; Iwatani, Chizuru; Tsuchiya, Hideaki; Okahara, Junko; Sankai, Tadashi; Torii, Ryuzo

doi:10.1016/j.theriogenology.2011.01.010

Cited by 33 publications

(32 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The procedures in cynomolgus monkeys for housing, oocyte collection, intra- cytoplasmic sperm injection (ICSI), pre-implantation embryo culture, and transfer of pre- implantation embryos into foster mothers were performed as described previously with some modifications 9, 11,12,13,14 . Briefly, monkeys were housed individually in appropriate cages, and the light cycle consisted of 12 h of artificial light from 8 AM to 8 PM.…”

Section: Methodsmentioning

confidence: 99%

Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys

et al. 2017

View full text Add to dashboard Cite

In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation development appears relatively similar, but the manner and morphology of post-implantation development are significantly different. Nevertheless, the embryogenesis just after implantation in primates, including the specification of PGCs, has been unexplored due to the difficulties in analyzing the embryos at relevant developmental stages. Here, we present a comprehensive single-cell transcriptome dataset of pre- and early post-implantation embryo cells, PGCs and embryonic stem cells (ESCs) of cynomolgus monkeys as a model of higher primates. The identities of each transcriptome were also validated rigorously by other way such as immunofluorescent analysis. The information reported here will serve as a foundation for our understanding of a wide range of processes in the developmental biology of primates, including humans.

show abstract

Section: Methodsmentioning

confidence: 99%

Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The day of vaginal plug detection was designated as embryonic day 0 (E0), and the day of birth as postnatal day 0 (P0). Embryonic cynomolgus monkeys were obtained by Caesarian section of artificially-inseminated embryos as previously reported (Yamasaki et al 2011). All animal experiments were carried out under the Guidelines for Laboratory Animals of Kumamoto University and Shiga University of Medical Science.…”

Section: Animalsmentioning

confidence: 99%

Developing guinea pig brain as a model for cortical folding

2017

View full text Add to dashboard Cite

The cerebral cortex in mammals, the neocortex specifically, is highly diverse among species with respect to its size and morphology, likely reflecting the immense adaptiveness of this lineage. In particular, the pattern and number of convoluted ridges and fissures, called gyri and sulci, respectively, on the surface of the cortex are variable among species and even individuals. However, little is known about the mechanism of cortical folding, although there have been several hypotheses proposed. Recent studies on embryonic neurogenesis revealed the differences in cortical progenitors as a critical factor of the process of gyrification. Here, we investigated the gyrification processes using developing guinea pig brains that form a simple but fundamental pattern of gyri. In addition, we established an electroporation-mediated gene transfer method for guinea pig embryos. We introduce the guinea pig brain as a useful model system to understand the mechanisms and basic principle of cortical folding.

show abstract

“…We injected 447 oocytes and transferred 63 blastocysts to recipient female monkeys, but because of the limited number of recipients, we needed to vitrify the remainder. Previously, no cynomolgus monkeys have been produced from cryopreserved blastocysts, while the use of cryopreserved embryos at earlier stages has succeeded [18,19]. When cryopreserved blastocysts were thawed, 26 out of 54 vitrified APPlentivirus injected blastocysts expanded successfully (48.1%), while some of them showed retarded growth (Fig.…”

Section: Resultsmentioning

confidence: 99%

Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein

Seita

Morimura

Watanabe

et al. 2020

JAD

View full text Add to dashboard Cite

Alzheimer's disease (AD) is the most common cause of dementia and understanding its pathogenesis should lead to improved therapeutic and diagnostic methods. Although several groups have developed transgenic mouse models overexpressing the human amyloid-␤ precursor protein (APP) gene with AD mutations, with and without presenilin mutations, as well as APP gene knock-in mouse models, these animals display amyloid pathology but do not show neurofibrillary tangles or neuronal loss. This presumably is due to differences between the etiology of the aged-related human disease and the mouse models. Here we report the generation of two transgenic cynomolgus monkeys overexpressing the human gene for APP with Swedish, Artic, and Iberian mutations, and demonstrated expression of gene tagged green fluorescent protein marker in the placenta, amnion, hair follicles, and peripheral blood. We believe that these nonhuman primate models will be very useful to study the pathogenesis of dementia and AD. However, generated Tg monkeys still have some limitations. We employed the CAG promoter, which will promote gene expression in a non-tissue specific manner. Moreover, we used transgenic models but not knock-in models. Thus, the inserted transgene destroys endogenous gene(s) and may affect the phenotype(s). Nevertheless, it will be of great interest to determine whether these Tg monkeys will develop tauopathy and neurodegeneration similar to human AD.

show abstract

Vitrification and transfer of cynomolgus monkey (Macaca fascicularis) embryos fertilized by intracytoplasmic sperm injection

Cited by 33 publications

References 12 publications

Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys

Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys

Developing guinea pig brain as a model for cortical folding

Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein

Contact Info

Product

Resources

About